anti-Mouse (Murine) IL-6 Receptor antibody for Western Blotting

Recommended IL-6 Receptor Antibody (supplied by: Log in to see )

Antigen
Interleukin-6 Receptor (IL6R) Antibodies
  • IL6R
  • il-6ra
  • CD126
  • IL-6R-1
  • IL-6RA
  • IL6Q
  • IL6RA
  • IL6RQ
  • gp80
  • IL6R1
  • Il6ra
  • IL-6Ralpha
  • IL-6R
  • Il6r
  • interleukin 6 receptor
  • interleukin 6 receptor, alpha
  • IL6R
  • il6r
  • Il6r
  • Il6ra
Epitope
AA 20-357
24
15
11
7
7
6
6
5
5
5
5
5
5
5
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
183
73
47
1
Host
Goat
123
102
35
15
1
1
1
Clonality
Polyclonal
Conjugate
This IL-6 Receptor antibody is un-conjugated
29
15
10
10
6
5
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Immunohistochemistry (IHC), Neutralization (Neut), Western Blotting (WB)
138
94
84
82
43
26
25
25
16
15
13
11
8
8
7
4
4
3
2
2
2
2
2
1
Supplier
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Catalog No. ABIN4899510
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.5 ABIN734693 IF (p) IHC (p) WB Rabbit IgG AA 386-430 Log in to see Polyclonal 2
10.808835 ABIN1175054 ICC IHC WB Rabbit IgG AA 205-347 Log in to see Polyclonal 0
10.808835 ABIN2935539 IF/ICC IHC IP WB Rabbit AA 205-347 Log in to see Polyclonal 0
7.808835 ABIN6262530 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
7.808835 ABIN1859439 ICC IHC WB Rabbit IgG AA 52-204 Log in to see Polyclonal 0
7.808835 ABIN2935540 IF/ICC IHC IP WB Rabbit AA 52-204 Log in to see Polyclonal 0
5.5 ABIN4899507 ELISA (Detection) FACS WB Biotin Goat IgG AA 20-357 Log in to see Polyclonal 3
4.808835 ABIN2000701 ELISA WB Rabbit IgG AA 44-357 Log in to see Polyclonal 1
4 ABIN2660934 ELISA (Capture) ELISPOT (Capture) ICFC Neut IHC WB Biotin Rat IgG2b kappa Log in to see D7715A7 11
4 ABIN2609956 IHC IHC (p) WB Rabbit IgG AA 205-347 Log in to see Polyclonal 0
1 ABIN2660933 ELISA (Capture) ELISPOT (Capture) ICFC Neut IHC WB Biotin Rat IgG2b kappa Log in to see D7715A7 11
1 ABIN734702 IHC (p) WB HRP Rabbit IgG AA 386-430 Log in to see Polyclonal 0
1 ABIN2609959 WB Rabbit IgG AA 52-204 Log in to see Polyclonal 0
1 ABIN327368 ELISA WB Rat IgG1 Extracellular Domain Log in to see 0
1 ABIN734695 IHC (p) WB Biotin Rabbit IgG AA 386-430 Log in to see Polyclonal 0
1 ABIN2624198 WB Biotin Rabbit IgG AA 52-204 Log in to see Polyclonal 0
1 ABIN2624200 WB Biotin Rabbit IgG AA 205-347 Log in to see Polyclonal 0
1 ABIN2624203 WB FITC Rabbit IgG AA 52-204 Log in to see Polyclonal 0
1 ABIN2624207 WB FITC Rabbit IgG AA 205-347 Log in to see Polyclonal 0
1 ABIN360909 IHC (fro) WB Rat IgG2 Extracellular Domain Log in to see 14F33 0

General

Antigen Interleukin-6 Receptor (IL6R) Antibodies
Epitope AA 20-357
(24), (15), (11), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(183), (73), (47), (1)
Host Goat
(123), (102), (35), (15), (1), (1), (1)
Clonality
Polyclonal
Conjugate This IL-6 Receptor antibody is un-conjugated
(29), (15), (10), (10), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Immunohistochemistry (IHC), Neutralization (Neut), Western Blotting (WB)
(138), (94), (84), (82), (43), (26), (25), (25), (16), (15), (13), (11), (8), (8), (7), (4), (4), (3), (2), (2), (2), (2), (2), (1)
Pubmed 9 references available
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Product Details anti-IL-6 Receptor Antibody

Target Details IL-6 Receptor Application Details Handling References for anti-IL-6 Receptor antibody (ABIN4899510) Images
Specificity Detects mouse IL-6 R alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.3 % cross-reactivity with recombinant mouse (rm) IL-6, recombinant human IL-6 R and rmgp130 is observed.
Purification Immunogen affinity purified
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen Mouse myeloma cell line NS0-derived recombinant mouse IL-6 R alpha , Leu20-Glu357, Accession # P22272
Isotype IgG
Plasmids, Primers & others

Target Details IL-6 Receptor

Product Details anti-IL-6 Receptor Antibody Application Details Handling References for anti-IL-6 Receptor antibody (ABIN4899510) Images back to top
Antigen
Alternative Name IL-6 R alpha (IL6R Antibody Abstract)
Gene ID 3570
UniProt P22272
Pathways JAK-STAT Signaling, Autophagy, Growth Factor Binding

Application Details

Product Details anti-IL-6 Receptor Antibody Target Details IL-6 Receptor Handling References for anti-IL-6 Receptor antibody (ABIN4899510) Images back to top
Application Notes Western Blot 0.1 μg/mL
Flow Cytometry 2.5 μg/10^6 cells
Immunohistochemistry 5-15 μg/mL
Neutralization 0.2-1 μg/mL
CyTOF-ready
ELISA Capture (Matched Antibody Pair) 0.2-0.8 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-IL-6 Receptor Antibody Target Details IL-6 Receptor Application Details References for anti-IL-6 Receptor antibody (ABIN4899510) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-IL-6 Receptor antibody (ABIN4899510)

Product Details anti-IL-6 Receptor Antibody Target Details IL-6 Receptor Application Details Handling Images back to top
Product cited in:

Owusu-Boaitey, Bauckman, Zhang, Mysorekar: "Macrophagic control of the response to uropathogenic E. coli infection by regulation of iron retention in an IL-6-dependent manner." in: Immunity, inflammation and disease, Vol. 4, Issue 4, pp. 413-426, 2018 Method employed by authors: Biochemical Assay (BCA) (Sample species: Mouse (Murine)).

Arnold, Boll, Rothaug, Schumacher, Schmidt, Wichert, Schneppenheim, Lokau, Pickhinke, Koudelka, Tholey, Rabe, Scheller, Lucius, Garbers, Rose-John, Becker-Pauly: "Meprin Metalloproteases Generate Biologically Active Soluble Interleukin-6 Receptor to Induce Trans-Signaling." in: Scientific reports, Vol. 7, pp. 44053, 2017

Wang, Miller, Goswami, Zhang, Ronning, Karlen, Zawadzki, Pugh, Burns: "Rapid monocyte infiltration following retinal detachment is dependent on non-canonical IL6 signaling through gp130." in: Journal of neuroinflammation, Vol. 14, Issue 1, pp. 121, 2017 Method employed by authors: Neutralization (Neut), Neutralization (Neut) (Sample species: Mouse (Murine)).

Garbers, Jänner, Chalaris, Moss, Floss, Meyer, Koch-Nolte, Rose-John, Scheller: "Species specificity of ADAM10 and ADAM17 proteins in interleukin-6 (IL-6) trans-signaling and novel role of ADAM10 in inducible IL-6 receptor shedding." in: The Journal of biological chemistry, Vol. 286, Issue 17, pp. 14804-11, 2011 Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

Rabe, Chalaris, May, Waetzig, Seegert, Williams, Jones, Rose-John, Scheller: "Transgenic blockade of interleukin 6 transsignaling abrogates inflammation." in: Blood, Vol. 111, Issue 3, pp. 1021-8, 2008 Method employed by authors: ELISA (ELISA), ELISA (ELISA), ELISA (ELISA) (Sample species: Mouse (Murine)).

Walker, Zhang, Matthews, Weinstock, Nice, Ernst, Rose-John, Burgess: "IL6/sIL6R complex contributes to emergency granulopoietic responses in G-CSF- and GM-CSF-deficient mice." in: Blood, Vol. 111, Issue 8, pp. 3978-85, 2008 Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

Chalaris, Rabe, Paliga, Lange, Laskay, Fielding, Jones, Rose-John, Scheller: "Apoptosis is a natural stimulus of IL6R shedding and contributes to the proinflammatory trans-signaling function of neutrophils." in: Blood, Vol. 110, Issue 6, pp. 1748-55, 2007 Method employed by authors: ELISA (ELISA), ELISA (ELISA), ELISA (ELISA) (Sample species: Mouse (Murine)).

Kurooka, Kaneda: "Inactivated Sendai virus particles eradicate tumors by inducing immune responses through blocking regulatory T cells." in: Cancer research, Vol. 67, Issue 1, pp. 227-36, 2007 Method employed by authors: In vivo Studies (in vivo), In vivo Studies (in vivo), In vivo Studies (in vivo) (Sample species: Mouse (Murine)).

Osuchowski, Siddiqui, Copeland, Remick: "Sequential ELISA to profile multiple cytokines from small volumes." in: Journal of immunological methods, Vol. 302, Issue 1-2, pp. 172-81, 2005 Method employed by authors: ELISA (ELISA), ELISA (ELISA), ELISA (ELISA) (Sample species: Mouse (Murine)).

Images

Product Details anti-IL-6 Receptor Antibody Target Details IL-6 Receptor Application Details Handling References for anti-IL-6 Receptor antibody (ABIN4899510) back to top
Supplier Images
Neutralization (Neut) image for anti-Interleukin-6 Receptor (IL6R) (AA 20-357) antibody (ABIN4899510) Cell Proliferation Induced by IL-6 and Neutralization by Mouse IL-6 R alpha Antibody....
Immunohistochemistry (IHC) image for anti-Interleukin-6 Receptor (IL6R) (AA 20-357) antibody (ABIN4899510) IL-6 R alpha in Mouse Thymus. IL-6 R alpha was detected in perfusion fixed frozen sec...
Immunohistochemistry (IHC) image for anti-Interleukin-6 Receptor (IL6R) (AA 20-357) antibody (ABIN4899510) IL-6 R alpha in Mouse Thymus. IL-6 R alpha was detected in perfusion fixed frozen sec...