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|Antigen||Leptin (LEP) Antibodies|
|Epitope||AA 131-145 Alternatives|
|Conjugate||This Leptin antibody is un-conjugated Alternatives|
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
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Product Details anti-Leptin AntibodyTarget Details Leptin Application Details Handling Images
|Specificity||Human Leptin. Binds to the carboxyl terminal region of the protein and to the intact leptin specifically in ELISA, Western blot and immunocytochemistry.|
|Cross-Reactivity (Details)||Cross-reactivity: Leptin (Human) 100 % Leptin (131-145) 100 % Epidermal Growth Factor (Human) 0 % Insulin (Human) 0 % Insulin-like Growth Factor 1 (Human) 0 % Insulin-like Growth Factor 2 (Rat) 0 % Parathyroid hormone (Human) 0 % Transforming Growth Factor-alpha (Human) 0 %|
|Characteristics||MAb to Leptin, Monoclonal antibody to Human Leptin, (AA 131-145)|
|Immunogen||Synthetic human leptin (131-145) attached to a carrier protein|
Target Details LeptinProduct Details anti-Leptin Antibody Application Details Handling Images back to top
|Alternative Name||Leptin (LEP Antibody Abstract)|
|Pathways||JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process|
Application DetailsProduct Details anti-Leptin Antibody Target Details Leptin Handling Images back to top
|Application Notes||Has been found to stain specifically human adipocytes at a 1:1000 dilution. The ability to bind to leptin in adipocytes was examined in cells fixed with neutral buffered formalin. The fixed cells were incubated for 20 minutes with 4 % normal goat serum, reacted for 60 minutes with the diluted monoclonal antibody and then with FITC-conjugated Goat anti-Mouse IgG. The immunofluorescent staining pattern was observed using epifluorescence microscopy. Western immunoblots of adipocyte homogenates resulted in a single band being detected at ~16 kDa at a dilution of 1:4000. Suitable for use in IHC using frozen sections. Paraffin-embedded sections have not been tested.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-Leptin Antibody Target Details Leptin Application Details Images back to top
Avoid multiple freeze/thaw cycles.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.
|Storage Comment||Upon receipt, store at -20 °C.|