PDX1 ELISA Kit
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- Target See all PDX1 ELISA Kits
- PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))
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Reactivity
- Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.15-10 ng/mL
- Minimum Detection Limit
- 0.15 ng/mL
- Application
- ELISA
- Brand
- BioAssay™
- Sensitivity
- < 0.053 ng/mL
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- Plate
- Pre-coated
- Protocol
- Insulin Promoter Factor 1 (IPF) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Promoter Factor 1 (IPF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Promoter Factor 1 (IPF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Promoter Factor 1 (IPF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Insulin Promoter Factor 1 (IPF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C/-20 °C
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- Target See all PDX1 ELISA Kits
- PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))
- Alternative Name
- Insulin Promoter Factor 1 (IPF) (PDX1 Products)
- Synonyms
- GSF ELISA Kit, IDX-1 ELISA Kit, IPF1 ELISA Kit, IUF1 ELISA Kit, MODY4 ELISA Kit, PDX-1 ELISA Kit, STF-1 ELISA Kit, ipf1 ELISA Kit, PDX1 ELISA Kit, IPF-1 ELISA Kit, Ipf1 ELISA Kit, Mody4 ELISA Kit, pdx-1 ELISA Kit, Idx1 ELISA Kit, Stf1 ELISA Kit, zgc:100874 ELISA Kit, XlHbox-8 ELISA Kit, xlhbox8 ELISA Kit, pancreatic and duodenal homeobox 1 ELISA Kit, pancreatic and duodenal homeobox 1 S homeolog ELISA Kit, PDX1 ELISA Kit, pdx1 ELISA Kit, Pdx1 ELISA Kit, pdx1.S ELISA Kit
- Pathways
- Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Hormone Transport, Carbohydrate Homeostasis, Chromatin Binding, Maintenance of Protein Location
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