AGT ELISA Kit

Catalog No. ABIN2702821

Product Details AGT ELISA Kit

Target: AGT (Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.22-300 ng/mL

Minimum Detection Limit: 1.22 ng/mL

Application: ELISA

Purpose: Human Angiotensinogen (SERPINA8) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificity: The antibody pair provided in this kit recognizes Human Angiotensinogen (AGT/SERPINA8)

Sensitivity: 1.22 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples100 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for AGT

Target: AGT (Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT))

Alternative Name: Angiotensinogen (AGT Products)

Synonyms: ANHU ELISA Kit, SERPINA8 ELISA Kit, AI265500 ELISA Kit, AngI ELISA Kit, AngII ELISA Kit, Aogen ELISA Kit, Serpina8 ELISA Kit, ANRT ELISA Kit, Ang ELISA Kit, PAT ELISA Kit, wu:fb62f06 ELISA Kit, wu:fj87b02 ELISA Kit, zgc:111892 ELISA Kit, AGT ELISA Kit, angt ELISA Kit, ANGT ELISA Kit, angiotensinogen ELISA Kit, angiotensinogen (serpin peptidase inhibitor, clade A, member 8) ELISA Kit, AGT ELISA Kit, Agt ELISA Kit, agt ELISA Kit

Background: Gene Names: AGT SERPINA8
Protein names: Angiotensinogen (Serpin A8) [Cleaved into: Angiotensin-1 (Angiotensin 1-10) (Angiotensin I) (Ang I), Angiotensin-2 (Angiotensin 1-8) (Angiotensin II) (Ang II), Angiotensin-3 (Angiotensin 2-8) (Angiotensin III) (Ang III) (Des-Asp[1]-angiotensin II), Angiotensin-4 (Angiotensin 3-8) (Angiotensin IV) (Ang IV), Angiotensin 1-9, Angiotensin 1-7, Angiotensin 1-5, Angiotensin 1-4]

Gene ID: 183

UniProt: P01019

Pathways: JAK-STAT Signaling, ACE Inhibitor Pathway, EGFR Signaling Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Feeding Behaviour, Monocarboxylic Acid Catabolic Process, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Regulation of long-term Neuronal Synaptic Plasticity