IL-1 beta ELISA Kit
Quick Overview for IL-1 beta ELISA Kit (ABIN5026930)
Target
See all IL-1 beta (IL1B) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 7.8 pg/mL
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Purpose
- This assay employs an antibody specific for anti-human IL-1b coated on a 96-well plate.
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Analytical Method
- Quantitative
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Sensitivity
- 4.69 pg/mL
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Characteristics
- This immunoassay kit allows for the in vitro quantitative determination of Human IL-1Beta concentrations in serum, plasma. Detection Range: 7.8 - 500 pg/mL Sensitivity: < 4.69 pg/mL
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Components
- plate, standard, diluent, antibodies
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Material not included
- pipettes, tubes, reader, incubator
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Interleukin 1, beta (IL1B) ELISA Kit
IL1B Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B Reactivity: Human AA 117-269 Colorimetric Sandwich ELISA 3.9 pg/mL - 250 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 1, beta (IL1B) ELISA KitIL1B Reactivity: Rat AA 117-268 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 1, beta (IL1B) ELISA KitIL1B Reactivity: Rabbit Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B Reactivity: Mouse AA 118-269 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 1, beta (IL1B) ELISA KitIL1B Reactivity: Human Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Cerebrospinal Fluid, Serum, Urine
Interleukin 1, beta (IL1B) ELISA KitIL1B Reactivity: Fish Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B Reactivity: Chicken Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Sample Volume
- 100 μL
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Assay Time
- 3 - 4 h
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Plate
- Pre-coated
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Protocol
- This assay employs an antibody specific for anti-human IL-1b coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1b present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-1Beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1b bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Restrictions
- For Research Use only
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Precaution of Use
- protect your eyes
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Storage
- 4 °C
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Storage Comment
- Please refer to the Manual
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- IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))
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Alternative Name
- IL-1 Beta (Interleukin 1 Beta)
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Pathways
- NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome
Target See all IL-1 beta (IL1B) ELISA Kits
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