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Bcl-2 ELISA Kit

BCL2 Reactivity: Human Colorimetric Sandwich ELISA 0.156-10 ng/mL Plasma, Serum
Catalog No. ABIN5026957
  • Target See all Bcl-2 (BCL2) ELISA Kits
    Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))
    Reactivity
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.156-10 ng/mL
    Minimum Detection Limit
    0.156 ng/mL
    Application
    ELISA
    Purpose
    This immunoassay kit allows for the in vitro quantitative determination of Human Bcl-2 concentrations in serum, plasma and other biological fluids.
    Sample Type
    Plasma, Serum
    Analytical Method
    Quantitative
    Sensitivity
    < 0.094 ng/mL
    Components
    plate, standard, diluent, antibodies
    Material not included
    pipettes, tubes, reader, incubator
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  • Sample Volume
    100 μL
    Assay Time
    3 - 4 h
    Plate
    Pre-coated
    Protocol
    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human Bcl-2 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human Bcl-2 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Bcl-2 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human Bcl-2 can be calculated using the standard curve.
    Restrictions
    For Research Use only
  • Precaution of Use
    protect your eyes
    Storage
    4 °C
  • Target See all Bcl-2 (BCL2) ELISA Kits
    Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))
    Alternative Name
    Bcl -2 (B cell Lymphoma 2) (BCL2 Products)
    Synonyms
    Bcl-2 ELISA Kit, PPP1R50 ELISA Kit, bcl-2 ELISA Kit, AW986256 ELISA Kit, C430015F12Rik ELISA Kit, D630044D05Rik ELISA Kit, D830018M01Rik ELISA Kit, BCL-2 ELISA Kit, Bcl2 ELISA Kit, BCL2, apoptosis regulator ELISA Kit, B cell leukemia/lymphoma 2 ELISA Kit, B-cell CLL/lymphoma 2a ELISA Kit, B-cell CLL/lymphoma 2 protein ELISA Kit, BCL2 ELISA Kit, Bcl2 ELISA Kit, bcl2a ELISA Kit, BCL-2 ELISA Kit
    Pathways
    MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
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