PD-L1 ELISA Kit
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- Target See all PD-L1 ELISA Kits
- PD-L1 (CD274 (PD-L1))
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Reactivity
- Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.156-10 ng/mL
- Minimum Detection Limit
- 0.156 ng/mL
- Application
- ELISA
- Purpose
- This immunoassay kit allows for the in vitro quantitative determination of Mouse PDL-1 concentrations in serum, plasma and other biological fluids.
- Sample Type
- Plasma, Serum
- Analytical Method
- Quantitative
- Sensitivity
- < 0.094 ng/mL
- Components
- plate, standard, diluent, antibodies
- Material not included
- pipettes, tubes, reader, incubator
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- Discover our best selling PD-L1 ELISA Kit
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- Discover our top product PD-L1 ELISA Kit
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CD274 (PD-L1) ELISA Kit
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- Sample Volume
- 100 μL
- Assay Time
- 3 - 4 h
- Plate
- Pre-coated
- Protocol
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Mouse PDL-1 antibody was pre-coated into 96-well plates. Biotin conjugated anti-MousePDL-1detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse PDL-1 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Mouse PDL-1 can be calculated using the standard curve.
- Restrictions
- For Research Use only
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- Precaution of Use
- protect your eyes
- Storage
- 4 °C
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- Target See all PD-L1 ELISA Kits
- PD-L1 (CD274 (PD-L1))
- Alternative Name
- PD-L1 (PD-L1 Products)
- Synonyms
- B7-H ELISA Kit, B7H1 ELISA Kit, PD-L1 ELISA Kit, PDCD1L1 ELISA Kit, PDCD1LG1 ELISA Kit, PDL1 ELISA Kit, A530045L16Rik ELISA Kit, B7h1 ELISA Kit, Pdcd1l1 ELISA Kit, Pdcd1lg1 ELISA Kit, Pdl1 ELISA Kit, RGD1566211 ELISA Kit, CD274 molecule ELISA Kit, CD274 antigen ELISA Kit, programmed cell death 1 ligand 2 ELISA Kit, CD274 ELISA Kit, Cd274 ELISA Kit, PDCD1LG2 ELISA Kit
- Pathways
- Cancer Immune Checkpoints
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