CD25 ELISA Kit

Catalog No. ABIN5520142

Product Details CD25 ELISA Kit

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 93.75 pg/mL - 6000 pg/mL

Minimum Detection Limit: 93.75 pg/mL

Application: ELISA

Purpose: For quantitative detection of CD25 in serum, plasma, tissue homogenates.

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of CD25. No significant cross-reactivity or interference between CD25 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between CD25 and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 56.25 pg/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

References for CD25 ELISA Kit (ABIN5520142)

Wang, Bi, Zhu, He, Lai, Xia, Cai: "IL-2Rα up-regulation is mediated by latent membrane protein 1 and promotes lymphomagenesis and chemotherapy resistance in natural killer/T-cell lymphoma." in: Cancer communications (London, England), Vol. 38, Issue 1, pp. 62, (2019) (PubMed).

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: CD25 (IL2RA Products)

Synonyms: CD25 ELISA Kit, IDDM10 ELISA Kit, IL2R ELISA Kit, TCGFR ELISA Kit, IL2RAC ELISA Kit, IL-2RA ELISA Kit, Il2r ELISA Kit, Ly-43 ELISA Kit, interleukin 2 receptor subunit alpha ELISA Kit, interleukin-2 receptor subunit alpha-like ELISA Kit, interleukin 2 receptor, alpha chain ELISA Kit, IL2RA ELISA Kit, Il2ra ELISA Kit, LOC100222957 ELISA Kit

Background: CD25/IL-2Rα/Interleukin 2 Receptor Alpha

UniProt: P01589

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation