TGFB1 ELISA Kit

Catalog No. ABIN5521662

Human TGFB1 ELISA Kit, Colorimetric assay for quantification of Human TGFB1, that has been mentioned in 1 publication.

Quick Overview for TGFB1 ELISA Kit (ABIN5521662)

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Product Details TGFB1 ELISA Kit

Minimum Detection Limit: 31.25 pg/mL

Purpose: Human TGF-β1 (Transforming Growth Factor Beta 1) ELISA Kit

Analytical Method: Quantitative

Specificity: Specifically binds with TGF-β1 , no obvious cross reaction with other analogues. Recommended Sample Dilution Ratio The following table shows the recommended dilution ratios for this kit for a limited number of samples for your reference only. (The matrix components in serum/plasma will affect the test results, which it need to be diluted at least 1/2 with Sample Dilution Buffer before testing! When the content of other samples is very low, the original solution can be added without dilution, but it is necessary to ensure that the pH is between 6.8 and 8.0, and it does not contain more than 10 % organic solvents or high-concentration protein denaturants.) 1. Common sample validation: Sample Type Recommended Dilution Ratio Content Healthy serum (n=25) 1/100-1/200 dilution 30.5-92 ng/mL Healthy plasma (EDTA,heparin) (n=25) 1/10-1/20 dilution 1.4-6.8 ng/mL Healthy Urine (n=8) 1/2 dilution ND-87pg/mL Human peripheral blood mononuclear cells were cultured with 5 %FBS + 1640 + double antibody, and the cell culture supernatant was detected. 1/5 dilution 2.2 ng/mL Hela cell were cultured with 5 %FBS + 1640 + double antibody, and the cell culture supernatant was detected. 1/5 dilution 3.7 ng/mL 2. KO sample validation (Detect TGF-β1 KO A549 cells): Sample Type Dilution Ratio Content Wild A549 cells were cultured with 10 %FBS + 1640 + double antibody, and the cell culture supernatant was detected. 1/5 dilution 1.6 ng/mL Wild A549 cells were cultured with 10 %FBS + 1640 + double antibody and then cultured with 300 ng/mL Brefeldin A (BFA) for 3 hours. Add cell lysis buffer(Catalogue No.:E050), collect the lysate solution (total protein concentration measured by BCA assay: 2.3 mg/mL) to detect. 1/5 dilution 2.8 ng/mg(total protein) KO A549 cells were cultured with 10 %FBS + 1640 + double antibody and then cultured with 300 ng/mL Brefeldin A (BFA) for 3 hours. Add cell lysis buffer(Catalogue No.:E050), collect the lysate solution (total protein concentration measured by BCA assay: 1.9 mg/mL) to detect. 1/2 dilution ND Note:ND is lower than the sensitivity of the kit and was not detected 3. Capture antibody and detection antibody by WB KO validation (Detect TGF-β1 KO A549 cells):

Sensitivity: 18.75 pg/mL

Components:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• Biotin-labeled Antibody
• HRP-Streptavidin Conjugate(SABC
• 100X)
• TMB Substrate
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Material not included:

• Microplate reader (wavelength: 450nm)
• 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Precision single (0.5-10μL
• 5-50μL
• 20-200μL
• 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Assay Time: 4 h

Plate: Pre-coated

Protocol: Step 1: Add 100 µL standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37 °C. Washing: Wash the plate twice without immersing. Step 2: Add 100 µL biotin-antibody working solution, seal the plate and statically incubate for 60 minutes at 37 °C. Washing: Wash the plate three times and immerse for 1 min each time. Step 3: Add 100 µL HRP-Streptavidin Conjugate (SABC) working solution, seal the plate and statically incubate for 30 minutes at 37 °C. Washing: Wash the plate five times and immerse for 1 min each time. Step 4: Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 5: Add 50 µL stop solution. Read at 450nm immediately and calculate.

Restrictions: For Research Use only

Handling

Handling Advice: Avoid direct light exposure to antibody solutions

Storage: 4 °C

Storage Comment: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Expiry Date: 12 months

References for TGFB1 ELISA Kit (ABIN5521662)

Sauriasari, Pratiwi: "Urinary TGF-β1 was not independently associated with renal function in diabetes mellitus." in: Diabetes, metabolic syndrome and obesity : targets and therapy, Vol. 11, pp. 597-602, (2018) (PubMed).

Target Details for TGFB1

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Alternative Name: TGFbeta1

Background: Transforming growth factor beta-1 proprotein, Latency-associated peptide, LAP, Transforming growth factor beta-1, TGF-beta-1, TGFB1, TGFB

UniProt: P01137

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints