CSF2 ELISA Kit (Colony Stimulating Factor 2 (Granulocyte-Macrophage))

Details for Product CSF2 ELISA Kit No. ABIN5653175, Supplier: Log in to see
Antigen
  • GMCSF
  • GM-CSF
  • CSF2
  • Csfgm
  • Gm-CSf
  • MGI-IGM
  • Gm-csf
  • Gmcsf
  • CSF
  • colony stimulating factor 2
  • colony stimulating factor 2 (granulocyte-macrophage)
  • CSF2
  • Csf2
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
31
23
21
12
7
5
4
3
3
2
2
1
Method Type
Sandwich ELISA
Detection Range
15.62 pg/mL - 1000 pg/mL
Minimum Detection Limit
15.62 pg/mL
Application
ELISA
Options
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Sample Type Plasma, Serum
Analytical Method Quantitative
Detection Method Chemiluminescent
Specificity This assay has high sensitivity and excellent specificity for detection of Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF). No significant cross-reactivity or interference between Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) and analogues was observed.
Sensitivity 0.56 pg/mL
Plasmids, Primers & others Plasmids, Primers & others CSF2 products on genomics-online (e.g. as negative or positive controls)
Alternative Name Colony Stimulating Factor 2, Granulocyte Macrophage (CSF2 ELISA Kit Abstract)
Background

Gene Name: Colony Stimulating Factor 2, Granulocyte Macrophage

Gene Aliases: CSF2, GM-CSF, Sargramostim, Molgramostin, Granulocyte-Macrophage Colony Stimulating Factor

Gene ID 12981
UniProt P01587
Pathways JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin
Comment

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time 2 - 3 h
Plate Pre-coated
Protocol The microplate provided in this kit has been pre-coated with an antibody specific to Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) level in the sample or standard.,
Assay Precision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Restrictions For Research Use only
Handling Advice Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Storage 4 °C,-20 °C
Storage Comment -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Expiry Date 4-8 months
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