WNT4 CLIA Kit

Catalog No. ABIN5660017

Product Details WNT4 CLIA Kit

Target: WNT4 (Wingless-Type MMTV Integration Site Family, Member 4 (WNT4))

Reactivity: Rat

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 156.25 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156.25 pg/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 4 (WNT4). No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 4 (WNT4) and analogues was observed.

Sensitivity: 5.29 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 4 (WNT4). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 4 (WNT4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Wingless Type MMTV Integration Site Family, Member 4 (WNT4) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 4 (WNT4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 4 (WNT4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: WNT4 (Wingless-Type MMTV Integration Site Family, Member 4 (WNT4))

Alternative Name: Wingless Type MMTV Integration Site Family, Member 4 (WNT4 Products)

Synonyms: CG4698 CLIA Kit, DWnt-4 CLIA Kit, DWnt4 CLIA Kit, Dm DWnt4 CLIA Kit, Dmel\\CG4698 CLIA Kit, Dwnt4 CLIA Kit, Wnt CLIA Kit, Wnt-4 CLIA Kit, anon-EST:Liang-2.4 CLIA Kit, clone 2.4 CLIA Kit, wnt-4 CLIA Kit, wnt4 CLIA Kit, SERKAL CLIA Kit, WNT-4 CLIA Kit, Xwnt4 CLIA Kit, xwnt-4 CLIA Kit, zgc:136737 CLIA Kit, Wnt family member 4 CLIA Kit, Wnt oncogene analog 4 CLIA Kit, wingless-type MMTV integration site family, member 4 CLIA Kit, wingless-type MMTV integration site family member 4 S homeolog CLIA Kit, wingless-type MMTV integration site family, member 4a CLIA Kit, WNT4 CLIA Kit, Wnt4 CLIA Kit, wnt4.S CLIA Kit, wnt4a CLIA Kit

Background:

Gene Name: Wingless Type MMTV Integration Site Family, Member 4

Pathways: WNT Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cell-Cell Junction Organization, Tube Formation