TRAF3 ELISA Kit
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- Target See all TRAF3 ELISA Kits
- TRAF3 (TNF Receptor-Associated Factor 3 (TRAF3))
- Binding Specificity
- Isoform 2
- Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- Human TRAF3 Isoform 2 ELISA Kit.
- Sample Type
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product TRAF3 ELISA Kit
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Plate
- Pre-coated
- Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Expiry Date
- 6 months
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- Target See all TRAF3 ELISA Kits
- TRAF3 (TNF Receptor-Associated Factor 3 (TRAF3))
- Alternative Name
- TRAF3 (TRAF3 Products)
- Synonyms
- CAP-1 ELISA Kit, CAP1 ELISA Kit, CD40bp ELISA Kit, CRAF1 ELISA Kit, IIAE5 ELISA Kit, LAP1 ELISA Kit, AI528849 ELISA Kit, T-BAM ELISA Kit, amn ELISA Kit, TNF receptor associated factor 3 ELISA Kit, TNF receptor-associated factor 3 ELISA Kit, Tnf receptor-associated factor 3 ELISA Kit, TRAF3 ELISA Kit, Traf3 ELISA Kit
- Gene ID
- 7187
- UniProt
- Q13114
- Pathways
- NF-kappaB Signaling, Apoptosis, TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades
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