anti-Human ARAF antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended ARAF Antibody (supplied by: Log in to see )

Antigen
V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) Antibodies
  • zgc:92074
  • wu:fk45h07
  • ARAF
  • a-raf
  • araf
  • araf1
  • pks2
  • rafa1
  • A-RAF
  • ARAF1
  • PKS2
  • RAFA1
  • 1200013E08Rik
  • A-Raf
  • AW495444
  • Araf1
  • A-Raf proto-oncogene, serine/threonine kinase
  • A-Raf proto-oncogene, serine/threonine kinase L homeolog
  • A-Raf proto-oncogene, serine/threonine kinase S homeolog
  • Araf proto-oncogene, serine/threonine kinase
  • araf
  • ARAF
  • araf.L
  • araf.S
  • Araf
Epitope
C-Term
24
23
16
15
11
10
10
10
9
7
5
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
167
99
87
6
6
6
4
2
2
2
1
Host
Rabbit
144
25
Clonality
Polyclonal
Conjugate
This ARAF antibody is un-conjugated
6
6
6
3
3
3
3
3
3
3
3
3
3
3
2
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
118
71
42
39
27
14
8
3
2
2
1
1
1
1
Options
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Catalog No. ABIN2855039
$ 466.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
17.117401 ABIN5557283 IF (cc) IHC (p) WB Rabbit IgG Log in to see 24D1 0
12.755681 ABIN5622540 ICC IF IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
7 ABIN392244 IHC (p) WB Rabbit Ig Fraction AA 256-286, Center Log in to see Polyclonal 1
7 ABIN2891222 ELISA IHC IHC (p) WB Rabbit IgG AA 276-325 Log in to see Polyclonal 0
7 ABIN5533640 IHC (p) WB Rabbit Ig Fraction AA 256-286 Log in to see Polyclonal 0
7 ABIN271994 IHC (p) WB Rabbit Log in to see Polyclonal 0
7 ABIN746078 IF (p) IHC (p) WB Rabbit IgG pTyr301, pTyr302 Log in to see Polyclonal 0
7 ABIN745328 IF (p) IHC (p) WB Rabbit IgG pSer299 Log in to see Polyclonal 0
7 ABIN5572937 ELISA IHC (p) WB Rabbit Log in to see Polyclonal 0
4 ABIN360127 EIA IHC (p) Rabbit Ig Fraction Middle Region Log in to see Polyclonal 4
1 ABIN2617218 IHC IHC (p) WB Mouse IgG1 AA 145-365 Log in to see 2F8 0
1 ABIN2617217 IHC IHC (p) WB Mouse IgG2a AA 145-365 Log in to see 2G9 0
1 ABIN1832873 ICC IHC IHC (p) WB Rabbit IgG AA 371-554 Log in to see Polyclonal 0
1 ABIN2883484 ELISA IF IHC IHC (p) WB Rabbit IgG pTyr302 Log in to see Polyclonal 0
1 ABIN2891750 IHC IHC (p) WB Rabbit Asp297 Log in to see Polyclonal 0
1 ABIN746087 IHC (p) WB HRP Rabbit IgG pTyr301, pTyr302 Log in to see Polyclonal 0
1 ABIN682777 IHC (p) WB HRP Rabbit IgG pSer214 Log in to see Polyclonal 0
1 ABIN745337 IHC (p) WB HRP Rabbit IgG pSer299 Log in to see Polyclonal 0
1 ABIN5958846 ELISA IHC (p) WB Rabbit IgG Tyr302 Log in to see Polyclonal 0
1 ABIN682770 IHC (p) WB Biotin Rabbit IgG pSer214 Log in to see Polyclonal 0

General

Antigen V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) Antibodies
Epitope C-Term
(24), (23), (16), (15), (11), (10), (10), (10), (9), (7), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(167), (99), (87), (6), (6), (6), (4), (2), (2), (2), (1)
Host Rabbit
(144), (25)
Clonality
Polyclonal
Conjugate This ARAF antibody is un-conjugated
(6), (6), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(118), (71), (42), (39), (27), (14), (8), (3), (2), (2), (1), (1), (1), (1)
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Product Details anti-ARAF Antibody

Target Details ARAF Application Details Handling Images
Cross-Reactivity Pig (Porcine), Rat (Rattus), Zebrafish (Danio rerio), Cow (Bovine)
Cross-Reactivity (Details) Pig (99 %), Rat (99 %), Zebrafish (85 %), Bovine (99 %)
Characteristics Rabbit Polyclonal antibody to A-RAF (v-raf murine sarcoma 3611 viral oncogene homolog)
A-RAF antibody [C1C3]
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the C-terminus region of human A-RAF. The exact sequence is proprietary.
Isotype IgG
Plasmids, Primers & others

Target Details ARAF

Product Details anti-ARAF Antibody Application Details Handling Images back to top
Antigen
Alternative Name A-RAF (ARAF Antibody Abstract)
Background This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development.

Molecular Weight 68 kDa
Gene ID 369
Pathways MAPK Signaling, RTK Signaling, Hepatitis C

Application Details

Product Details anti-ARAF Antibody Target Details ARAF Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3

Restrictions For Research Use only

Handling

Product Details anti-ARAF Antibody Target Details ARAF Application Details Images back to top
Format Liquid
Concentration 0.85 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-ARAF Antibody Target Details ARAF Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (C-Term) antibody (ABIN2855039) ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed A431, using ARAF, ...
Western Blotting (WB) image for anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (C-Term) antibody (ABIN2855039) WB Image Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE antibody diluted a...
Western Blotting (WB) image for anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (C-Term) antibody (ABIN2855039) WB Image Sample (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE antibody diluted...
Immunohistochemistry (IHC) image for anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (C-Term) antibody (ABIN2855039) IHC-P Image Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ...