anti-Human MAP3K5 antibody for ELISA

Recommended MAP3K5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
  • ASK1
  • MAPKKK5
  • MEKK5
  • mitogen-activated protein kinase kinase kinase 5
  • MUD21.11
  • MUD21_11
  • 7420452D20Rik
  • ASK
  • Mekk5
  • Ask1
  • RGD1306565
  • mitogen-activated protein kinase kinase kinase 5
  • MAP3K5
  • MAPKKK5
  • Map3k5
  • map3k5
Reactivity
Human
400
177
97
5
1
1
Host
Mouse
379
27
Clonality
Monoclonal
Conjugate
This MAP3K5 antibody is un-conjugated
11
11
10
5
5
5
5
5
5
5
5
5
5
5
4
4
4
2
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
312
172
134
83
65
45
20
13
12
11
8
4
2
1
1
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Catalog No. ABIN969273
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
10.60976 ABIN4281875 ELISA FACS ICC IF IHC WB Mouse IgG1 Log in to see 2E4 0
10.60976 ABIN1844371 ELISA FACS IF/ICC IHC WB Mouse IgG1 Log in to see 0
10.60976 ABIN517859 IF ELISA WB Mouse IgG Mix lambda AA 1231-1374, partial Log in to see 2D11 0
10.60976 ABIN95283 IP IHC RIA ELISA WB Rabbit IgG AA 76-87, pSer83 Log in to see Polyclonal 0
10.60976 ABIN517858 ELISA WB Mouse IgG2b kappa AA 1231-1374, partial Log in to see 1E3 0
10.60976 ABIN517860 ELISA WB Mouse IgG Mix lambda AA 1231-1374, partial Log in to see 2E6 0
1 ABIN1532802 IHC ELISA WB Rabbit IgG AA 932-981 Log in to see Polyclonal 2
1 ABIN1531773 IHC ELISA WB Rabbit IgG AA 932-981, pSer966 Log in to see Polyclonal 2
1 ABIN1532801 IHC ELISA WB Rabbit IgG AA 49-98 Log in to see Polyclonal 0
1 ABIN1531772 IHC ELISA WB Rabbit IgG AA 49-98, pSer83 Log in to see Polyclonal 0
1 ABIN1496745 ELISA FACS IF IHC WB Mouse IgG1 Log in to see 20000 0
1 ABIN2878953 ELISA IHC IHC (p) WB Rabbit IgG AA 49-98 Log in to see Polyclonal 0
1 ABIN966515 FACS IF IHC ELISA WB Mouse IgG1 Log in to see 2E4 3
1 ABIN2888359 ELISA IHC IHC (p) WB Rabbit IgG pSer966 Log in to see Polyclonal 0
1 ABIN2888358 ELISA IHC IHC (p) WB Rabbit IgG pSer83 Log in to see Polyclonal 0
1 ABIN2888947 ELISA IHC WB Rabbit IgG AA 932-981 Log in to see Polyclonal 0
1 ABIN461898 ELISA IHC IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0
1 ABIN461897 ELISA ICC IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN210987 ELISA WB Rabbit pSer83 Log in to see Polyclonal 0
1 ABIN3181921 ELISA IHC WB Rabbit IgG pSer966 Log in to see Polyclonal 0

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
Reactivity Human
(400), (177), (97), (5), (1), (1)
Host Mouse
(379), (27)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAP3K5 antibody is un-conjugated
(11), (11), (10), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (2)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(312), (172), (134), (83), (65), (45), (20), (13), (12), (11), (8), (4), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-MAP3K5 Antibody

Target Details MAP3K5 Application Details Handling References for anti-MAP3K5 antibody (ABIN969273) Images
Purification Ascites
Immunogen Purified recombinant fragment of human MAP3K5 expressed in E. Coli.
Clone 2-00E-04
Isotype IgG1
Plasmids, Primers & others

Target Details MAP3K5

Product Details anti-MAP3K5 Antibody Application Details Handling References for anti-MAP3K5 antibody (ABIN969273) Images back to top
Antigen
Alternative Name MAP3K5 (MAP3K5 Antibody Abstract)
Background Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells, MAPKKK5 does not activate MAPK/ERK.
Molecular Weight 55 kDa
Gene ID 4217
HGNC 4217
Pathways MAPK Signaling, Positive Regulation of Endopeptidase Activity, Unfolded protein response

Application Details

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Handling References for anti-MAP3K5 antibody (ABIN969273) Images back to top
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400
Restrictions For Research Use only

Handling

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details References for anti-MAP3K5 antibody (ABIN969273) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage

References for anti-MAP3K5 antibody (ABIN969273)

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling Images back to top
Product cited in:

Widenmaier, Ao, Kim, Warnock, McIntosh et al.: "Suppression of p38 MAPK and JNK via Akt-mediated inhibition of apoptosis signal-regulating kinase 1 constitutes a core component of the beta-cell pro-survival effects of glucose-dependent ..." in: The Journal of biological chemistry, Vol. 284, Issue 44, pp. 30372-82, 2009

Images

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling References for anti-MAP3K5 antibody (ABIN969273) back to top
Supplier Images
ELISA image for anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) antibody (ABIN969273) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) antibody (ABIN969273) Figure 2: Flow cytometric analysis of Hela cells using MAP3K5 mouse mAb (green) and n...
Immunocytochemistry (ICC) image for anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) antibody (ABIN969273) Figure 3: Immunofluorescence analysis of Hela cells using MAP3K5 mouse mAb (green) . ...
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) antibody (ABIN969273) Figure 4: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (le...
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) antibody (ABIN969273) Figure 5: Western blot analysis using MAP3K5 mAb against human MAP3K5 (AA: 922-1108) ...