anti-Human MAP3K5 antibody for Immunofluorescence

Recommended MAP3K5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
  • ASK1
  • MAPKKK5
  • MEKK5
  • mitogen-activated protein kinase kinase kinase 5
  • MUD21.11
  • MUD21_11
  • 7420452D20Rik
  • ASK
  • Mekk5
  • Ask1
  • RGD1306565
  • mitogen-activated protein kinase kinase kinase 5
  • MAP3K5
  • MAPKKK5
  • Map3k5
  • map3k5
Epitope
AA 1231-1374
65
53
30
22
19
17
15
15
15
11
9
8
7
6
6
5
5
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
406
179
100
5
1
1
Host
Mouse
384
27
Clonality
Monoclonal
Conjugate
This MAP3K5 antibody is un-conjugated
11
11
10
5
5
5
5
5
5
5
5
5
5
5
4
4
4
2
Application
ELISA, Immunofluorescence (IF), Western Blotting (WB)
317
169
137
81
65
50
26
14
12
11
8
4
2
1
1
Options
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Catalog No. ABIN393594
$ 461.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
10.57848 ABIN615252 EIA IF IHC (p) WB Rabbit C-Term Log in to see Polyclonal 0
10.57848 ABIN4281869 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal 0
10.57848 ABIN5583027 ELISA FACS IF IHC (p) WB Mouse IgG1 Log in to see 2E4 0
10.57848 ABIN517859 IF ELISA WB Mouse IgG Mix lambda AA 1231-1374, partial Log in to see 2D11 0
10.57848 ABIN1108200 EIA IF IHC (p) WB Mouse IgG1 Log in to see 2-00E-004 1
10.57848 ABIN4281878 ELISA ICC IF IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal 0
10.57848 ABIN5545337 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
10.57848 ABIN4948308 IF IHC (p) WB Rabbit Log in to see Polyclonal 0
1 ABIN1496745 ELISA FACS IF IHC WB Mouse IgG1 Log in to see 20000 0
1 ABIN6255571 ELISA ICC IF IHC WB Rabbit IgG pSer966 Log in to see Polyclonal 1
1 ABIN197310 IF IHC (p) WB Rabbit Ser83 Log in to see Polyclonal 3
1 ABIN197326 IF IHC (p) WB Rabbit Ser966 Log in to see Polyclonal 4
1 ABIN5949321 FACS ICC IF IHC IHC (p) WB Rabbit IgG AA 1-200 Log in to see SU36-06 0
1 ABIN966515 FACS IF IHC ELISA WB Mouse IgG1 Log in to see 2E4 3
1 ABIN362730 IF IHC Rabbit IgG AA 964-968 Log in to see Polyclonal 5
1 ABIN4281875 ELISA FACS ICC IF IHC WB Mouse IgG1 Log in to see 2E4 0
1 ABIN318975 IHC (fro) IF WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN362714 IF IHC WB Rabbit IgG AA 81-85 Log in to see Polyclonal 4
1 ABIN6271358 ELISA ICC IF WB Rabbit IgG pThr838 Log in to see Polyclonal 0
1 ABIN6256431 ELISA ICC IF IHC WB Rabbit IgG pSer83 Log in to see Polyclonal 0

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
Epitope AA 1231-1374
(65), (53), (30), (22), (19), (17), (15), (15), (15), (11), (9), (8), (7), (6), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(406), (179), (100), (5), (1), (1)
Host Mouse
(384), (27)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAP3K5 antibody is un-conjugated
(11), (11), (10), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (2)
Application ELISA, Immunofluorescence (IF), Western Blotting (WB)
(317), (169), (137), (81), (65), (50), (26), (14), (12), (11), (8), (4), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-MAP3K5 Antibody

Target Details MAP3K5 Application Details Handling References for anti-MAP3K5 antibody (ABIN393594) Images
Specificity MAP3K5 (AAH54503, 1231 a.a. ~ 1374 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Cross-Reactivity (Details) Human: ~~ Positive
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen

Antigen Type: Recombinant Protein

Target/Specificity: MAP3K5 (AAH54503, 1231 a.a. ~ 1374 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone 2D11
Isotype IgG Mix lambda
Plasmids, Primers & others

Target Details MAP3K5

Product Details anti-MAP3K5 Antibody Application Details Handling References for anti-MAP3K5 antibody (ABIN393594) Images back to top
Antigen
Alternative Name MAP3K5 (MAP3K5 Antibody Abstract)
Background

Other Names: Mitogen-activated protein kinase kinase kinase 5, Apoptosis signal-regulating kinase 1, ASK-1, MAPK/ERK kinase kinase 5, MEK kinase 5, MEKK 5, MAP3K5, ASK1, MAPKKK5, MEKK5

Gene Description: mitogen-activated protein kinase kinase kinase 5

Gene Summary: Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells, MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq

Molecular Weight 154537 DA
Gene ID 4217
NCBI Accession NP_005914
UniProt Q99683
Pathways MAPK Signaling, Positive Regulation of Endopeptidase Activity, Unfolded protein response

Application Details

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Handling References for anti-MAP3K5 antibody (ABIN393594) Images back to top
Application Notes Western blot = 1:500-1000
Comment

Background: Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells, MAPKKK5 does not activate MAPK/ERK.

Restrictions For Research Use only

Handling

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details References for anti-MAP3K5 antibody (ABIN393594) Images back to top
Buffer Clear, colorless solution in phosphate buffered saline,  pH 7.2 .
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

References for anti-MAP3K5 antibody (ABIN393594)

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling Images back to top
Background publications

Bailey, Xie, Do, Montpetit, Diaz, Mohan, Keavney, Yusuf, Gerstein, Engert, Anand: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010

Yang, Yu, Guan, Gu, Cao, Wang, Zheng, Xu, Shen, Liu: "ASK1-JNK signaling cascade mediates Ad-ST13-induced apoptosis in colorectal HCT116 cells." in: Journal of cellular biochemistry, Vol. 110, Issue 3, pp. 581-8, 2010

Rose, Behm, Drgon, Johnson, Uhl: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010

Sturchler, Feurstein, McDonald, Duckett: "Mechanism of oxidative stress-induced ASK1-catalyzed MKK6 phosphorylation." in: Biochemistry, Vol. 49, Issue 19, pp. 4094-102, 2010

Mo, Jung, Yoon, Hong, Kim, Ann, Seo, Choi, Park: "DJ-1 modulates the p38 mitogen-activated protein kinase pathway through physical interaction with apoptosis signal-regulating kinase 1." in: Journal of cellular biochemistry, Vol. 110, Issue 1, pp. 229-37, 2010

Images

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling References for anti-MAP3K5 antibody (ABIN393594) back to top
Supplier Images
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 1231-1374) antibody (ABIN393594) Immunofluorescence of monoclonal antibody to MAP3K5 on A-431 cell (antibody concentra...
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 1231-1374) antibody (ABIN393594) anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 1231-1374) antibody (Image 2)
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 1231-1374) antibody (ABIN393594) Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunoge...
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 1231-1374) antibody (ABIN393594) MAP3K5 monoclonal antibody (M03), clone 2D11 Western Blot analysis of MAP3K5 expressi...
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 1231-1374) antibody (ABIN393594) Western Blot analysis of MAP3K5 expression in transfected 293T cell line by MAP3K5 mo...
Immunofluorescence (IF) image for anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 1231-1374) antibody (ABIN393594) Immunofluorescence of monoclonal antibody to MAP3K5 on A-431 cell. [antibody concentr...
ELISA image for anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 1231-1374) antibody (ABIN393594) Detection limit for recombinant GST tagged MAP3K5 is approximately 0.3ng/ml as a capt...