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|Antigen||Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies|
|Epitope||AA 325-345 Alternatives|
|Reactivity||Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MAPK3 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
|4 references available|
Product Details anti-MAPK3 AntibodyTarget Details MAPK3 Application Details Handling References for anti-MAPK3 antibody (ABIN967952) Images
|Cross-Reactivity||Mouse (Murine), Human, Chicken, Dog (Canine), Frog|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Rat ERK1 aa. 325-345|
|Plasmids, Primers & others|
Target Details MAPK3Product Details anti-MAPK3 Antibody Application Details Handling References for anti-MAPK3 antibody (ABIN967952) Images back to top
|Alternative Name||ERK1 (MAPK3 Antibody Abstract)|
|Background||The family of serine/threonine kinases known as ERKs (extracellular signal regulated kinases) or MAPKs (mitogen-activated protein kinases) is activated after cell stimulation by a variety of hormones and growth factors. Cell stimulation induces a signaling cascade that leads to phosphorylation of MEK (MAPK/ERK kinase) which, in turn, activates ERK via tyrosine and threonine phosphorylation. A myriad of proteins represent the downstream effectors for the active ERK and implicate it in the control of cell proliferation and differentiation, as well as regulation of the cytoskeleton. Activation of ERK is normally transient and cells possess dual specificity phosphotases that are responsible for its down-regulation. Furthermore, multiple studies have shown that elevated ERK activity is associated with some cancers. ERK1 is the 44 kDa member of the ERK family and shares 85% homology with ERK2 (42 kD).|
|Molecular Weight||44 kDa|
|Pathways||MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals|
Application DetailsProduct Details anti-MAPK3 Antibody Target Details MAPK3 Handling References for anti-MAPK3 antibody (ABIN967952) Images back to top
Related Products: ABIN968545, ABIN967389
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAPK3 Antibody Target Details MAPK3 Application Details References for anti-MAPK3 antibody (ABIN967952) Images back to top
|Buffer||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at -20° C.|
References for anti-MAPK3 antibody (ABIN967952)Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Handling Images back to top
|Product cited in:||
Radoja, Saio, Schaer, Koneru, Vukmanovic, Frey: "CD8(+) tumor-infiltrating T cells are deficient in perforin-mediated cytolytic activity due to defective microtubule-organizing center mobilization and lytic granule exocytosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 9, pp. 5042-51, 2001
Clark, Hynes: "Ras activation is necessary for integrin-mediated activation of extracellular signal-regulated kinase 2 and cytosolic phospholipase A2 but not for cytoskeletal organization." in: The Journal of biological chemistry, Vol. 271, Issue 25, pp. 14814-8, 1996
Cobb, Goldsmith: "How MAP kinases are regulated." in: The Journal of biological chemistry, Vol. 270, Issue 25, pp. 14843-6, 1995
Boulton, Cobb: "Identification of multiple extracellular signal-regulated kinases (ERKs) with antipeptide antibodies." in: Cell regulation, Vol. 2, Issue 5, pp. 357-71, 1991
ImagesProduct Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Handling References for anti-MAPK3 antibody (ABIN967952) back to top