anti-Human MAPT antibody for Western Blotting

Recommended MAPT Antibody

Antigen
Microtubule-Associated Protein tau (MAPT) Antibodies
  • DDPAC
  • FTDP-17
  • MAPTL
  • MSTD
  • MTBT1
  • MTBT2
  • PPND
  • TAU
  • AI413597
  • AW045860
  • Mtapt
  • Tau
  • RNPTAU
  • pTau
  • tau
  • xtp
  • MAPT
  • PHF-tau
  • slc6a6
  • taut
  • wu:fc26e12
  • microtubule associated protein tau
  • microtubule-associated protein tau
  • microtubule associated protein tau S homeolog
  • Microtubule-associated protein tau
  • solute carrier family 6 (neurotransmitter transporter), member 6b
  • MAPT
  • Mapt
  • mapt.S
  • LOC5580230
  • CpipJ_CPIJ013260
  • tau
  • slc6a6b
Epitope
pSer199
56
51
46
46
44
43
43
39
38
36
36
35
27
24
21
18
16
16
15
15
14
14
12
12
11
10
10
10
10
9
9
9
9
9
9
8
8
8
7
7
6
6
6
6
5
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1225
803
729
98
39
27
26
20
16
11
8
5
4
4
3
3
3
3
3
2
1
1
1
1
1
Host
Rabbit
1165
100
15
7
Clonality
Polyclonal
Conjugate
This MAPT antibody is un-conjugated
47
21
21
21
21
21
21
12
11
6
6
6
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
1056
449
326
107
83
62
28
21
18
14
13
12
12
12
8
4
2
1
Options

Available images

Catalog No. ABIN1573962
$ 160.67
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
23.450115 ABIN2854882 IHC (p) WB Rabbit IgG Center Polyclonal 0
23.450115 ABIN3020340 IF IHC WB Rabbit pSer262 Polyclonal 0
23.450115 ABIN3019704 IF IHC WB Rabbit pSer404 Polyclonal 0
21.950115 ABIN1574008 ELISA WB Rabbit IgG Ser422 Polyclonal 1
20.5 ABIN6652127 IHC (p) WB Rabbit pSer396 Polyclonal 15
20.450115 ABIN3187181 ELISA WB Rabbit IgG Ser855 Polyclonal 0
20.450115 ABIN3187182 ELISA IF IHC WB Rabbit IgG Ser86 Polyclonal 0
20.450115 ABIN3182162 ELISA WB Rabbit IgG pSer396 Polyclonal 0
20.450115 ABIN1573963 ELISA WB Rabbit IgG pThr217 Polyclonal 0
20.450115 ABIN1573981 ELISA WB Rabbit IgG Polyclonal 0
20.450115 ABIN6254081 IF WB Chicken Polyclonal 0
20.450115 ABIN3019680 IF WB Rabbit pSer356 Polyclonal 0
20.450115 ABIN3019698 IHC WB Rabbit pThr205 Polyclonal 0
20.450115 ABIN3019683 IHC WB Rabbit pSer396 Polyclonal 0
20.450115 ABIN3020346 IHC WB Rabbit pThr181 Polyclonal 0
20.450115 ABIN3020352 IHC WB Rabbit pThr231 Polyclonal 0
20.450115 ABIN2443721 IF IHC WB Mouse IgG1 AA 12-24, pTyr18 9G3 0
20.450115 ABIN6559941 IHC ELISA WB Rabbit pSer208, pSer210 Polyclonal 0
20.450115 ABIN6559939 IHC ELISA WB Rabbit pThr175 Polyclonal 0
20.450115 ABIN6559940 IHC ELISA WB Rabbit pThr217 Polyclonal 0

General

Antigen Microtubule-Associated Protein tau (MAPT) Antibodies
Epitope pSer199
(56), (51), (46), (46), (44), (43), (43), (39), (38), (36), (36), (35), (27), (24), (21), (18), (16), (16), (15), (15), (14), (14), (12), (12), (11), (10), (10), (10), (10), (9), (9), (9), (9), (9), (9), (8), (8), (8), (7), (7), (6), (6), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1225), (803), (729), (98), (39), (27), (26), (20), (16), (11), (8), (5), (4), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(1165), (100), (15), (7)
Clonality
Polyclonal
Conjugate This MAPT antibody is un-conjugated
(47), (21), (21), (21), (21), (21), (21), (12), (11), (6), (6), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(1056), (449), (326), (107), (83), (62), (28), (21), (18), (14), (13), (12), (12), (12), (8), (4), (2), (1)
Pubmed 1 reference available
Supplier

Product Details anti-MAPT Antibody

Target Details MAPT Application Details Handling References for anti-MAPT antibody (ABIN1573962) Images
Specificity Rabbit Anti-Tau (Phospho-Ser199) Polyclonal Antibody detects endogenous levels of Tau only when phosphorylated at serine 199.
Purification The antibody was purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide and cross-adsorbed with the corresponding non-phosphopeptide.
Immunogen Synthetic phospho-peptide (KLH-coupled) corresponding - AA surrounding pSer199 of human Tau
Isotype IgG
Plasmids, Primers & others

Target Details MAPT

Product Details anti-MAPT Antibody Application Details Handling References for anti-MAPT antibody (ABIN1573962) Images back to top
Antigen
Alternative Name tau (MAPT Antibody Abstract)
Background Tau is a microtubule-associated phosphoprotein (MAP), localized in neuronal axons. It promotes tubulin polymerization and stabilizes microtubules. Tau proteins constitute a family of six isoforms which range from 352 to 441 amino acids. The tau variants differ from each other by the presence of either three or four repeat-regions in the carboxy-terminal part of the molecule and the absence or presence of one or two inserts in the amino-terminal part. Tau is hyperphosphorylated by ERK, GSK-3, TPKII and CDK5, at least thirty phosphorylation sites have been described, including Thr39, Ser46, Thr50, Thr69, Thr153, Thr175, Thr 181, Ser198, Ser199, Ser202, Thr205, Ser208, Ser210, Thr212, Ser214, Thr217, Thr231, Ser235, Ser237, Ser241, Ser262, Ser285, Ser305, Ser324, Ser352, Ser356, Ser396, Ser400, Thr403, Ser404, Ser409, Ser412, Ser413, Ser416 and Ser422. Specifically, TPKII phosphorylates serines 202 and 404. GSK-3beta transfection phosphorylates serines 199, 202, 235, 396, 404 and 413, and threonines 205 and 231. These sites are among the major abnormal phosphorylation sites of Tau. Phosphorylation on these sites reduces the ability of a given Tau species to promote microtubule self-assembly. Hyperphosphorylated Tau is the major protein of the paired helical filaments (PHFs), which make up the pathological neurofibrillary tangles of Alzheimer-disease (AD). The PHFs are also found in the lesions of other central nervous system disorders.Rabbit Anti-Tau (Phospho-Ser199) Polyclonal Antibody is developed in rabbit using a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser 199 of human Tau.
Pathways MAPK Signaling, Microtubule Dynamics, M Phase, Regulation of Cell Size

Application Details

Product Details anti-MAPT Antibody Target Details MAPT Handling References for anti-MAPT antibody (ABIN1573962) Images back to top
Application Notes Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1 µg/mL
Other applications: user-optimized
Restrictions For Research Use only

Handling

Product Details anti-MAPT Antibody Target Details MAPT Application Details References for anti-MAPT antibody (ABIN1573962) Images back to top
Format Liquid
Buffer PBS, pH 7.4, containing 30 % glycerol, and 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C/-20 °C
Storage Comment The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References for anti-MAPT antibody (ABIN1573962)

Product Details anti-MAPT Antibody Target Details MAPT Application Details Handling Images back to top
Product cited in:

Jamal, Nita-Lazar, Gao, Amin, Walker, Kukuruzinska: "N-glycosylation status of E-cadherin controls cytoskeletal dynamics through the organization of distinct ?-catenin- and ?-catenin-containing AJs." in: Cell health and cytoskeleton, Vol. 2009, Issue 1, pp. 67-80, 2011

Images

Product Details anti-MAPT Antibody Target Details MAPT Application Details Handling References for anti-MAPT antibody (ABIN1573962) back to top
Supplier Images
Western Blotting (WB) image for anti-Microtubule-Associated Protein tau (MAPT) (pSer199) antibody (ABIN1573962) Western blot analysis of mouse brain tissue lysate using Rabbit Anti-Tau (Phospho-Ser...
Western Blotting (WB) image for anti-Microtubule-Associated Protein tau (MAPT) (pSer199) antibody (ABIN1573962) Western blot analysis of rat brain lysates using 1 µg/mL Antibodies-Online Rabbit Ant...