anti-Mouse (Murine) PPARG antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended PPARG Antibody (supplied by: Log in to see )

Antigen
Peroxisome Proliferator-Activated Receptor gamma (PPARG) Antibodies
  • PPAR gamma
  • PPARg2
  • PPARG
  • Nr1c3
  • PPAR-gamma
  • PPAR-gamma2
  • PPARgamma
  • PPARgamma2
  • NR1C3
  • CIMT1
  • GLM1
  • PPARG1
  • PPARG2
  • xPPAR-gamma
  • PPAR-GAMMA
  • PPARGAMMA
  • peroxisome proliferator-activated receptor gamma
  • peroxisome proliferator activated receptor gamma
  • peroxisome proliferator activated receptor gamma L homeolog
  • PPARG
  • Pparg
  • pparg.L
Epitope
AA 250-300, pSer273
39
28
17
17
16
15
14
12
11
9
8
6
5
5
5
5
5
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
302
162
128
31
24
15
13
12
11
10
6
6
5
5
5
5
4
4
3
2
2
2
1
1
1
Host
Rabbit
279
46
5
5
Clonality
Polyclonal
Conjugate
This PPARG antibody is un-conjugated
20
15
9
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
278
133
88
46
38
30
25
21
18
12
9
6
4
3
2
2
Options
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Catalog No. ABIN1387919
$ 340.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1386283 FACS IF (p) IHC (p) WB Rabbit IgG AA 170-220 Log in to see Polyclonal 1
1 ABIN703405 IF (p) IHC (p) WB Rabbit IgG AA 90-130, pSer112 Log in to see Polyclonal 1
1 ABIN874060 IF (p) Alexa Fluor 647 Rabbit IgG AA 90-130, pSer112 Log in to see Polyclonal 1
1 ABIN1396899 IF (p) Alexa Fluor 488 Rabbit IgG AA 250-300, pSer273 Log in to see Polyclonal 0
1 ABIN1396900 IF (p) Alexa Fluor 555 Rabbit IgG AA 250-300, pSer273 Log in to see Polyclonal 0
1 ABIN1396901 IF (p) Alexa Fluor 647 Rabbit IgG AA 250-300, pSer273 Log in to see Polyclonal 0
1 ABIN1396903 IF (p) FITC Rabbit IgG AA 250-300, pSer273 Log in to see Polyclonal 0
1 ABIN1396898 IF (p) Alexa Fluor 350 Rabbit IgG AA 250-300, pSer273 Log in to see Polyclonal 0
1 ABIN1396311 FACS IF (p) Alexa Fluor 488 Rabbit IgG AA 170-220 Log in to see Polyclonal 0
1 ABIN1396312 FACS IF (p) Alexa Fluor 555 Rabbit IgG AA 170-220 Log in to see Polyclonal 0
1 ABIN1396313 FACS IF (p) Alexa Fluor 647 Rabbit IgG AA 170-220 Log in to see Polyclonal 0
1 ABIN1396315 FACS IF (p) FITC Rabbit IgG AA 170-220 Log in to see Polyclonal 0
1 ABIN1396310 FACS IF (p) Alexa Fluor 350 Rabbit IgG AA 170-220 Log in to see Polyclonal 0
1 ABIN1414775 IF (p) Cy5 Rabbit IgG AA 250-300, pSer273 Log in to see Polyclonal 0
1 ABIN1414774 IF (p) Cy3 Rabbit IgG AA 250-300, pSer273 Log in to see Polyclonal 0
1 ABIN1414776 IF (p) Cy5.5 Rabbit IgG AA 250-300, pSer273 Log in to see Polyclonal 0
1 ABIN874061 IF (p) Alexa Fluor 488 Rabbit IgG AA 90-130, pSer112 Log in to see Polyclonal 0
1 ABIN874063 IF (p) Alexa Fluor 555 Rabbit IgG AA 90-130, pSer112 Log in to see Polyclonal 0
1 ABIN874064 IF (p) Alexa Fluor 350 Rabbit IgG AA 90-130, pSer112 Log in to see Polyclonal 0
1 ABIN1414188 FACS IF (p) Cy5.5 Rabbit IgG AA 170-220 Log in to see Polyclonal 0

General

Antigen Peroxisome Proliferator-Activated Receptor gamma (PPARG) Antibodies
Epitope AA 250-300, pSer273
(39), (28), (17), (17), (16), (15), (14), (12), (11), (9), (8), (6), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(302), (162), (128), (31), (24), (15), (13), (12), (11), (10), (6), (6), (5), (5), (5), (5), (4), (4), (3), (2), (2), (2), (1), (1), (1)
Host Rabbit
(279), (46), (5), (5)
Clonality
Polyclonal
Conjugate This PPARG antibody is un-conjugated
(20), (15), (9), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(278), (133), (88), (46), (38), (30), (25), (21), (18), (12), (9), (6), (4), (3), (2), (2)
Pubmed 10 references available
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Product Details anti-PPARG Antibody

Target Details PPARG Application Details Handling References for anti-PPARG antibody (ABIN1387919) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic phosphopeptide derived from human PPAR Gamma around the phosphorylation site of ser273
Isotype IgG
Plasmids, Primers & others

Target Details PPARG

Product Details anti-PPARG Antibody Application Details Handling References for anti-PPARG antibody (ABIN1387919) Images back to top
Antigen
Alternative Name PPAR gamma (PPARG Antibody Abstract)
Background

Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses.

Subcellular location: Cytoplasm, Nucleus

Synonyms: GLM1, CIMT1, NR1C3, PPARG1, PPARG2, PPARgamma, Peroxisome proliferator-activated receptor gamma, PPAR-gamma, Nuclear receptor subfamily 1 group C member 3, PPARG

Gene ID 5468
UniProt P37231
Pathways MAPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-PPARG Antibody Target Details PPARG Handling References for anti-PPARG antibody (ABIN1387919) Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-PPARG Antibody Target Details PPARG Application Details References for anti-PPARG antibody (ABIN1387919) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-PPARG antibody (ABIN1387919)

Product Details anti-PPARG Antibody Target Details PPARG Application Details Handling Images back to top
Product cited in:

Liu, Wan, Lang, Si, Zhu, Zhou, Mi: "Dihydromyricetin delays the onset of hyperglycemia and ameliorates insulin resistance without excessive weight gain in Zucker diabetic fatty rats." in: Molecular and cellular endocrinology, Vol. 439, pp. 105-115, 2018 Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)). Further details: Sample: Adipose

Liu, Zhou, Lang, Zhou, Mi: "Dihydromyricetin enhances glucose uptake by inhibition of MEK/ERK pathway and consequent down-regulation of phosphorylation of PPARγ in 3T3-L1 cells." in: Journal of cellular and molecular medicine, Vol. 22, Issue 2, pp. 1247-1256, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Abd Alla, Graemer, Fu, Quitterer: "Inhibition of G-protein-coupled Receptor Kinase 2 Prevents the Dysfunctional Cardiac Substrate Metabolism in Fatty Acid Synthase Transgenic Mice." in: The Journal of biological chemistry, Vol. 291, Issue 6, pp. 2583-600, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)). Further details: Sample: cardiac tissue

Agrawal, Chanley, Westbrook, Nie, Kitao, Guess, Benndorf, Hidalgo, Smoyer: "Pioglitazone Enhances the Beneficial Effects of Glucocorticoids in Experimental Nephrotic Syndrome." in: Scientific reports, Vol. 6, pp. 24392, 2016 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Rat (Rattus)). Further details: Sample: Kidney Glomeruli

Maganti, Tersey, Syed, Nelson, Colvin, Maier, Mirmira: "Peroxisome Proliferator-activated Receptor-γ Activation Augments the β-Cell Unfolded Protein Response and Rescues Early Glycemic Deterioration and β Cell Death in Non-obese Diabetic Mice." in: The Journal of biological chemistry, Vol. 291, Issue 43, pp. 22524-22533, 2016 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

Stechschulte, Ge, Hinds, Sanchez, Franceschi, Lecka-Czernik et al.: "Protein Phosphatase PP5 Controls Bone Mass and the Negative Effects of Rosiglitazone on Bone through Reciprocal Regulation of PPARγ (Peroxisome Proliferator-activated Receptor γ) and RUNX2 ..." in: The Journal of biological chemistry, Vol. 291, Issue 47, pp. 24475-24486, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)). Further details: Sample: Bone

Liu, Feng, Zhu, Liu, Han, Chen, Wang, Li, Li, Xu, Si: "Identification of a novel selective agonist of PPAR? with no promotion of adipogenesis and less inhibition of osteoblastogenesis." in: Scientific reports, Vol. 5, pp. 9530, 2015 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Kolli, Stechschulte, Dowling, Rahman, Czernik, Lecka-Czernik: "Partial agonist, telmisartan, maintains PPAR? serine 112 phosphorylation, and does not affect osteoblast differentiation and bone mass." in: PLoS ONE, Vol. 9, Issue 5, pp. e96323, 2014 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Pandurangan, Park, Kim: "Aspartame downregulates 3T3-L1 differentiation." in: In vitro cellular & developmental biology. Animal, Vol. 50, Issue 9, pp. 851-7, 2014 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Sarr, Thompson, Zhao, Lee, Regnault: "Low birth weight male guinea pig offspring display increased visceral adiposity in early adulthood." in: PLoS ONE, Vol. 9, Issue 6, pp. e98433, 2014 Method employed by authors: Western Blotting (WB) (Sample species: Guinea Pig).

Images

Product Details anti-PPARG Antibody Target Details PPARG Application Details Handling References for anti-PPARG antibody (ABIN1387919) back to top
Supplier Images
SDS-PAGE (SDS) image for anti-Peroxisome Proliferator-Activated Receptor gamma (PPARG) (AA 250-300), (pSer273) antibody (ABIN1387919) Lane 1: mouse lung lysates Lane 2: mouse stomach lysates probed with Anti Phospho-PPA...
Western Blotting (WB) image for anti-Peroxisome Proliferator-Activated Receptor gamma (PPARG) (AA 250-300), (pSer273) antibody (ABIN1387919) Image kindly submitted by Dr. Leckaczernik from the University of Toledo. AD2 cells w...