anti-Pig (Porcine) Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 antibody for Western Blotting

Recommended Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 Antibody

Antigen
Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1) Antibodies
  • ARO
  • ARO1
  • CPV1
  • CYAR
  • CYP19
  • CYPXIX
  • P-450AROM
  • Aromatase
  • Cyp19
  • Cyp19a
  • p450arom
  • P450 arom
  • aro
  • cyp19
  • cyp19a
  • p450arom-A
  • P450AROM
  • Ar
  • ArKO
  • Int-5
  • Int5
  • CYP19P1
  • CYP19A1
  • CYP19a1
  • P450aromA
  • ar1
  • cypXIX
  • etID309862.18
  • zgc:136541
  • cyp19a1
  • cytochrome P450 family 19 subfamily A member 1
  • cytochrome P450, family 19, subfamily a, polypeptide 1
  • cytochrome P450 family 19 subfamily A member 1 L homeolog
  • aromatase
  • cytochrome P450 aromatase
  • cytochrome P450, family 19, subfamily A, polypeptide 1
  • cytochrome P450 19A1
  • cytochrome P450, family 17, subfamily A, polypeptide 1
  • cytochrome P450, family 19
  • cytochrome P450, family 19, subfamily A, polypeptide 1a
  • cytochrome P450 19A1-like
  • CYP19A1
  • Cyp19a1
  • cyp19a1.L
  • SAV_2383
  • pfcyp19b-tstr
  • CYP19
  • cyp19a1a
  • LOC101174651
Reactivity
Chicken, Cow (Bovine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
160
85
59
7
5
4
4
3
3
3
3
2
2
2
2
1
1
1
Host
Rabbit
128
24
21
Clonality
Polyclonal
Conjugate
Un-conjugated
6
4
4
4
4
4
4
4
3
2
2
2
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
122
59
52
32
26
19
14
7
7
6
4
4
Options

Available images

Catalog No. ABIN223293
$ 320.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
9.390189 ABIN2473179 IF IHC (p) WB Mouse IgG2a H4 3
7 ABIN351538 IHC IHC (p) WB Rabbit Polyclonal 0
1 ABIN1819739 IF IHC IHC (p) WB Mouse IgG2a AA 376-390 H4 0
-9.163526 ABIN5682489 IHC IHC (p) WB Rabbit Polyclonal 0
-13.053715 ABIN5682490 ICC IF IHC WB Mouse IgG2a AA 376-390 [H4] 0
-13.053715 ABIN5682494 ICC IF IHC WB Alexa Fluor 700 Mouse IgG2a AA 376-390 [H4] 0
-13.053715 ABIN5682495 ICC IF IHC WB Biotin Mouse IgG2a AA 376-390 [H4] 0
-13.053715 ABIN5682498 ICC IF IHC WB DyLight 488 Mouse IgG2a AA 376-390 [H4] 0
-13.053715 ABIN5682501 ICC IF IHC WB DyLight 650 Mouse IgG2a AA 376-390 [H4] 0
-13.053715 ABIN5682493 ICC IF IHC WB Alexa Fluor 647 Mouse IgG2a AA 376-390 [H4] 0
-13.053715 ABIN5682497 ICC IF IHC WB DyLight 405 Mouse IgG2a AA 376-390 [H4] 0
-13.053715 ABIN5682499 ICC IF IHC WB DyLight 550 Mouse IgG2a AA 376-390 [H4] 0
-13.053715 ABIN5682491 ICC IF IHC WB Alexa Fluor 405 Mouse IgG2a AA 376-390 [H4] 0
-13.053715 ABIN5682496 ICC IF IHC WB DyLight 350 Mouse IgG2a AA 376-390 [H4] 0
-13.053715 ABIN5682500 ICC IF IHC WB DyLight 594 Mouse IgG2a AA 376-390 [H4] 0
-13.053715 ABIN5682492 ICC IF IHC WB Alexa Fluor 488 Mouse IgG2a AA 376-390 [H4] 0
-13.053715 ABIN5682502 ICC IF IHC WB DyLight 680 Mouse IgG2a AA 376-390 [H4] 0
-13.053715 ABIN5682503 ICC IF IHC WB DyLight 755 Mouse IgG2a AA 376-390 [H4] 0
-13.053715 ABIN5682505 IHC WB HRP Mouse IgG2a AA 376-390 [H4] 0

General

Antigen Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1) Antibodies
Reactivity Chicken, Cow (Bovine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(160), (85), (59), (7), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(128), (24), (21)
Clonality
Polyclonal
Conjugate Un-conjugated
(6), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(122), (59), (52), (32), (26), (19), (14), (7), (7), (6), (4), (4)
Pubmed 5 references available
Supplier

Product details

Target Details Application Details Handling References Images
Blocking Peptide Blocking peptide for this product available: ABIN412600
Specificity A 55 kDa band can be detected, corresponding to the expected molecular weight of Aromatase.
Immunogen Synthetic peptide surrounding amino acid 385 of human Aromatase
Isotype IgG

Target Details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name Aromatase (CYP19A1 Antibody Abstract)
Background Aromatase is a key enzyme in steroidogenesis and plays an important role in sexual differentiation, fertility, and carcinogenesis. Many environmental chemicals may influence aromatase activity and thereby disrupt endocrine function.
Synonyms: 25147 , Cyp19a1, Aromatase , Cyp19 , p450arom , ARO1, ARO 1, CPV, CYP19A1 , MGC104309
Gene ID 1588
UniProt P11511
Pathways Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Carbohydrate Homeostasis

Application Details

Product details Target Details Handling References Images back to top
Application Notes Western blotting (0.5-4 μg/mL), Immunohistochemistry (10-20 μg/mL). However, the optimal concentrations should be determined individually. Jurkat cell lysate can be used as a positive control. The antibody should recognize aromatase in samples from human, mouse, rat, bovine, porcine, pig, sheep, rabbit, horse and chicken origins. A 55 kDa band can be detected, corresponding to the expected molecular weight of Aromatase. An additional 35 kDa band (believe to be the cleavage product of Aromatase) can also be detected in Jurkat cells.
Restrictions For Research Use only

Handling

Product details Target Details Application Details References Images back to top
Format Liquid
Concentration 0.2 mg/mL
Buffer 100 μg (0.2 mg/mL) affinity purified rabbit anti-Aromatase polyclonal antibody in phosphate-buffered saline (PBS) containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References

Product details Target Details Application Details Handling Images back to top
Product cited in:

El Hajj Chehadeh, Dreumont, Willekens, Canabady-Rochelle, Jeannesson, Alberto, Daval, Guéant, Leininger-Muller: "Early methyl donor deficiency alters cAMP signaling pathway and neurosteroidogenesis in the cerebellum of female rat pups." in: American journal of physiology. Endocrinology and metabolism, Vol. 307, Issue 11, pp. E1009-19, 2014

Yonezawa, Higashi, Yoshioka, Mutoh: "Distribution of aromatase and sex steroid receptors in the baculum during the rat life cycle: effects of estrogen during the early development of the baculum." in: Biology of reproduction, Vol. 85, Issue 1, pp. 105-12, 2011

Bukulmez, Hardy, Carr, Word, Mendelson: "Inflammatory status influences aromatase and steroid receptor expression in endometriosis." in: Endocrinology, Vol. 149, Issue 3, pp. 1190-204, 2008

Hirayama, Sawai, Moriyasu, Hirayama, Goto, Kaneko, Miyamoto, Ushizawa, Takahashi, Minamihashi: "Excess estrogen sulfoconjugation as the possible cause for a poor sign of parturition in pregnant cows carrying somatic cell clone fetuses." in: Reproduction (Cambridge, England), Vol. 136, Issue 5, pp. 639-47, 2008

Sun, Yan, Jacobson, Jiang, Carroll, Huang: "Contribution of epoxyeicosatrienoic acids to flow-induced dilation in arteries of male ERalpha knockout mice: role of aromatase." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 293, Issue 3, pp. R1239-46, 2007

Images

Product details Target Details Application Details Handling References back to top
Supplier Images
Western Blotting (WB) image for anti-Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1) antibody (ABIN223293) Western blot analysis of Aromatase in human brain cell lysate.