anti-Dog (Canine) REST antibody for Western Blotting

Recommended REST Antibody (supplied by: Log in to see )

Antigen
RE1-Silencing Transcription Factor (REST) Antibodies
  • NRSF
  • XBR
  • 2610008J04Rik
  • AA407358
  • REST
  • xbr
  • nrsf
  • rest
  • RE1 silencing transcription factor
  • RE1-silencing transcription factor
  • REST
  • Rest
  • rest
Epitope
Middle Region
13
9
8
6
5
4
4
2
2
2
2
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
70
10
9
3
3
3
3
3
2
1
1
1
Host
Rabbit
62
7
2
Clonality
Polyclonal
Conjugate
This REST antibody is un-conjugated
4
4
3
2
2
2
Application
Western Blotting (WB)
58
28
12
10
9
8
3
2
1
1
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Catalog No. ABIN2779670
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.061829 ABIN2780696 WB Rabbit Middle Region Log in to see Polyclonal 0
3.061829 ABIN5518090 WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN798477 WB Rabbit AA 20-69 Log in to see Polyclonal 0

General

Antigen RE1-Silencing Transcription Factor (REST) Antibodies
Epitope Middle Region
(13), (9), (8), (6), (5), (4), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
(70), (10), (9), (3), (3), (3), (3), (3), (2), (1), (1), (1)
Host Rabbit
(62), (7), (2)
Clonality
Polyclonal
Conjugate This REST antibody is un-conjugated
(4), (4), (3), (2), (2), (2)
Application Western Blotting (WB)
(58), (28), (12), (10), (9), (8), (3), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-REST Antibody

Target Details REST Application Details Handling References for anti-REST antibody (ABIN2779670) Images
Blocking Peptide Blocking peptide for this product available: ABIN982856
Sequence PMLPPSAVEE REAVSKTALA SPPATMAANE SQEIDEDEGI HSHEGSDLSD
Predicted Reactivity Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 93%
Characteristics This is a rabbit polyclonal antibody against REST. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human REST
Plasmids, Primers & others

Target Details REST

Product Details anti-REST Antibody Application Details Handling References for anti-REST antibody (ABIN2779670) Images back to top
Antigen
Alternative Name REST (REST Antibody Abstract)
Background REST is a transcriptional repressor which represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells, and it is thought that this repressor may act as a master negative regular of neurogenesis.This gene encodes a transcriptional repressor which represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells, and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described, however, their full length nature has not been determined. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: NRSF, XBR
Protein Interaction Partner: BTRC, UBC, FBXW11, CSNK1E, NEDD8, EHMT2, EIF4A3, FBXW4, FBXW7, FBXW5, FBXW8, FBXW2, SIN3A, TERF2, GRIA2, SUB1, GTF2B, Tbp, SIN3B, HDAC1, RCOR1, SP1, SMARCE1, SMARCC2, SMARCA4, CDYL, HDAC5, HDAC4, HTT, NCOR1, KDM5C, RNF2, HDAC2, STMN2, GRM2, CTDSP1,
Protein Size: 1097
Molecular Weight 122 kDa
Gene ID 5978
NCBI Accession NM_005612, NP_005603
UniProt Q13127
Pathways Negative Regulation of Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Chromatin Binding, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-REST Antibody Target Details REST Handling References for anti-REST antibody (ABIN2779670) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 1097 AA

Restrictions For Research Use only

Handling

Product Details anti-REST Antibody Target Details REST Application Details References for anti-REST antibody (ABIN2779670) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-REST antibody (ABIN2779670)

Product Details anti-REST Antibody Target Details REST Application Details Handling Images back to top
Product cited in:

Mehta, Kim, Vemuganti: "Long Noncoding RNA FosDT Promotes Ischemic Brain Injury by Interacting with REST-Associated Chromatin-Modifying Proteins." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 50, pp. 16443-9, 2015

Images

Product Details anti-REST Antibody Target Details REST Application Details Handling References for anti-REST antibody (ABIN2779670) back to top
Supplier Images
Western Blotting (WB) image for anti-RE1-Silencing Transcription Factor (REST) (Middle Region) antibody (ABIN2779670) WB Suggested Anti-REST Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Posit...
Western Blotting (WB) image for anti-RE1-Silencing Transcription Factor (REST) (Middle Region) antibody (ABIN2779670) anti-RE1-Silencing Transcription Factor (REST) (Middle Region) antibody (Image 2)
Western Blotting (WB) image for anti-RE1-Silencing Transcription Factor (REST) (Middle Region) antibody (ABIN2779670) REST antibody - middle region validated by WB using A549 and HEK293T cells at 1: 500.
Western Blotting (WB) image for anti-RE1-Silencing Transcription Factor (REST) (Middle Region) antibody (ABIN2779670) Host: Rabbit Target Name: REST Sample Tissue: Human MCF7 Antibody Dilution: 1....
Western Blotting (WB) image for anti-RE1-Silencing Transcription Factor (REST) (Middle Region) antibody (ABIN2779670) Host: Rabbit Target Name: REST Sample Type: MCF7 Antibody Dilution: 1.0ug/ml ...
Western Blotting (WB) image for anti-RE1-Silencing Transcription Factor (REST) (Middle Region) antibody (ABIN2779670) Host: Rabbit Target Name: REST Sample Type: Human Fetal Liver Antibody Dilution...