anti-Human IKBKB antibody for Immunofluorescence

Recommended IKBKB Antibody (supplied by: Log in to see )

Antigen
Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) Antibodies
  • ikk-beta
  • ikk2
  • ikkb
  • ikkbeta
  • IKBKB
  • IKKB
  • CG4201
  • DIK
  • DLAK
  • DmIKK
  • DmIKKBeta
  • DmIKKb
  • DmIKKbeta
  • DmIkkbeta
  • Dmel\\CG4201
  • Dmikkb
  • Dmikkbeta
  • IK
  • IKK
  • IKK-beta
  • IKKb
  • IKKbeta
  • IRD-5
  • IRD5
  • Ik
  • Ikkb
  • Ikkbeta
  • Ird-5
  • Ird5
  • LRK
  • Lak
  • anon-89Bd
  • dIKK
  • dIKK-beta
  • dIKKbeta
  • dLak
  • dmIKKbeta
  • ikk
  • ird
  • ird5/DmIkk[beta]
  • IKK2
  • NFKBIKB
  • AIM-1
  • AI132552
  • IKK-2
  • IKK[b]
  • inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta L homeolog
  • inhibitor of nuclear factor kappa B kinase subunit beta
  • I-kappaB kinase beta
  • inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
  • inhibitor of kappaB kinase beta
  • ikbkb.L
  • IKBKB
  • ikbkb
  • IKKbeta
  • Ikbkb
Epitope
C-Term
58
32
29
23
21
21
20
20
20
20
20
20
20
19
19
19
19
17
17
17
15
8
8
7
7
7
6
6
5
4
4
4
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
685
227
158
22
13
7
7
6
5
4
1
1
Host
Rabbit
577
122
9
2
Clonality
Polyclonal
Conjugate
This IKBKB antibody is un-conjugated
38
37
37
33
33
31
9
7
5
5
5
5
5
5
5
5
5
2
2
2
2
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
341
307
264
107
81
77
68
42
37
33
6
6
6
5
3
3
3
2
1
Options
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Catalog No. ABIN2856162
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.745464 ABIN2707591 IC IF IHC WB Rabbit N-Term, pSer23 Log in to see Polyclonal 0
11.745464 ABIN442231 ICC IF IHC (p) IHC WB Rabbit IgG C-Term Log in to see Polyclonal 0
11.745464 ABIN4324266 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
11.745464 ABIN1873179 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
11.745464 ABIN1882097 IF IHC (p) WB Rabbit Log in to see Polyclonal 8
11.745464 ABIN1870261 IF Rabbit IgG pTyr199 Log in to see Polyclonal 0
11.745464 ABIN4324265 ELISA ICC IF WB Rabbit Log in to see Polyclonal 0
11.745464 ABIN516987 IF ELISA WB Mouse IgG2b kappa AA 3-120, partial Log in to see 3C12 0
11.745464 ABIN3031320 IF IHC ELISA WB Rabbit Ig Fraction Log in to see Polyclonal 0
11.745464 ABIN3020259 IF Rabbit pTyr199 Log in to see Polyclonal 0
11.745464 ABIN349146 IF Rabbit IgG pTyr199 Log in to see Polyclonal 0
11.745464 ABIN349358 IF Rabbit IgG Log in to see Polyclonal 0
1 ABIN252473 CyTOF FACS ICC IF IHC IHC (p) IP SimWes WB Mouse IgG1 Log in to see 10AG2 59
1 ABIN252477 FACS GS ICC IF IP SimWes WB Mouse IgG1 kappa Log in to see 10A9B6 9
1 ABIN401636 IF Rabbit Tyr199 Log in to see Polyclonal 1
1 ABIN4324294 FACS ICC IF IHC IHC (p) WB Alexa Fluor 647 Mouse IgG1 Log in to see 10AG2 0
1 ABIN218607 IF IHC (p) IP ELISA WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN5554863 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN5689462 IF WB Rabbit AA 197-201 Log in to see Polyclonal 0
1 ABIN302820 IF IHC (p) WB Rabbit Log in to see Polyclonal 0

General

Antigen Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) Antibodies
Epitope C-Term
(58), (32), (29), (23), (21), (21), (20), (20), (20), (20), (20), (20), (20), (19), (19), (19), (19), (17), (17), (17), (15), (8), (8), (7), (7), (7), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(685), (227), (158), (22), (13), (7), (7), (6), (5), (4), (1), (1)
Host Rabbit
(577), (122), (9), (2)
Clonality
Polyclonal
Conjugate This IKBKB antibody is un-conjugated
(38), (37), (37), (33), (33), (31), (9), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(341), (307), (264), (107), (81), (77), (68), (42), (37), (33), (6), (6), (6), (5), (3), (3), (3), (2), (1)
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Product Details anti-IKBKB Antibody

Target Details IKBKB Application Details Handling Images
Cross-Reactivity Rhesus Monkey
Cross-Reactivity (Details) Rhesus Monkey (100 %)
Characteristics Rabbit Polyclonal antibody to IKK beta (inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta)
IKK beta antibody
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the C-terminus region of human IKK beta. The exact sequence is proprietary.
Isotype IgG

Target Details IKBKB

Product Details anti-IKBKB Antibody Application Details Handling Images back to top
Antigen
Alternative Name IKK beta (IKBKB Antibody Abstract)
Background NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).[supplied by OMIM]

Cellular Localization: Cytoplasm
Molecular Weight 87 kDa
Gene ID 3551
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades

Application Details

Product Details anti-IKBKB Antibody Target Details IKBKB Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:10000*
Comment

Positive Control: A431 , PC-12 , DDDDK-human IKBKB-transfected 293T

Restrictions For Research Use only

Handling

Product Details anti-IKBKB Antibody Target Details IKBKB Application Details Images back to top
Format Liquid
Concentration 0.41 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-IKBKB Antibody Target Details IKBKB Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) (C-Term) antibody (ABIN2856162) WB Image IKK beta antibody detects IKK beta protein by western blot analysis. A. 30 μ...
Western Blotting (WB) image for anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) (C-Term) antibody (ABIN2856162) WB Image IKK beta antibody detects IKBKB protein by Western blot analysis. A. 30 µg P...
Immunofluorescence (IF) image for anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) (C-Term) antibody (ABIN2856162) ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed A431, using IKK be...
Immunohistochemistry (IHC) image for anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) (C-Term) antibody (ABIN2856162) IHC-P Image Immunohistochemical analysis of paraffin-embedded human colon carcinoma, ...
Western Blotting (WB) image for anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) (C-Term) antibody (ABIN2856162) WB Image Sample (30 ug of whole cell lysate) A: A431 , 7.5% SDS PAGE antibody diluted...