anti-Cow (Bovine) Insulin antibody for Western Blotting

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • INS-IGF2
  • Ins2
Reactivity
Cow (Bovine), Human, Pig (Porcine)
895
404
287
276
215
134
53
9
7
6
5
5
4
2
2
1
1
1
1
1
1
1
Host
Mouse
727
193
50
16
3
1
Clonality
Monoclonal
Conjugate
This Insulin antibody is un-conjugated
48
38
38
23
22
22
22
17
17
14
14
14
14
14
14
14
14
10
9
9
9
8
8
8
5
5
5
5
5
5
2
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
499
387
270
243
203
195
160
133
79
66
32
25
24
15
7
6
5
4
4
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN487416
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.185168 ABIN1172907 ICC IHC WB Rabbit IgG AA 25-54 Log in to see Polyclonal 0
1 ABIN272321 EIA IHC (p) WB Mouse IgG1 Log in to see E2E3 3
1 ABIN4326102 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see SPM139 0
1 ABIN2742155 ELISA FACS IF IHC IHC (p) IP WB Mouse IgG1, kappa AA 1-84 Log in to see 2D11-H5 or INS05 0
1 ABIN602181 IHC ELISA WB Mouse IgG1 Log in to see 0
1 ABIN4326054 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E2-E3 (same as INS04) 0
1 ABIN4326055 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E2-E3 (same as INS04) 0
1 ABIN4326058 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05) 0
1 ABIN4326053 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E2-E3 (same as INS04) 0
1 ABIN4326056 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05) 0
1 ABIN4326057 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05) 0
1 ABIN2742157 FACS IF IHC IHC (p) IP WB Mouse IgG1, kappa AA 1-84 Log in to see E2-E3-2D11-H5 or INS04-INS05 0
1 ABIN2742156 ELISA FACS IF IHC IHC (p) IP WB Mouse IgG1, kappa AA 1-84 Log in to see E2-E3 or INS04 0
1 ABIN4326090 FACS ICC IF IHC (fro) IHC (p) WB DyLight 650 Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05) 0
1 ABIN4326068 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04) 0
1 ABIN4326097 FACS ICC IF IHC (fro) IHC (p) WB DyLight 550 Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05) 0
1 ABIN4326067 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04) 0
1 ABIN4326069 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04) 0
1 ABIN4326073 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 650 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04) 0
1 ABIN4326078 WB PerCP Mouse IgG1 kappa Log in to see E2-E3 (same as INS04) 0

General

Antigen Insulin (INS) Antibodies
Reactivity Cow (Bovine), Human, Pig (Porcine)
(895), (404), (287), (276), (215), (134), (53), (9), (7), (6), (5), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(727), (193), (50), (16), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Insulin antibody is un-conjugated
(48), (38), (38), (23), (22), (22), (22), (17), (17), (14), (14), (14), (14), (14), (14), (14), (14), (10), (9), (9), (9), (8), (8), (8), (5), (5), (5), (5), (5), (5), (2), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(499), (387), (270), (243), (203), (195), (160), (133), (79), (66), (32), (25), (24), (15), (7), (6), (5), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling Images
Specificity The antibody recognizes Insulin and recombinant Proinsulin, but does not react with synthetic C-Peptide.
Characteristics Synonyms: INS
Purification Affinity Chromatography on Protein G.
Immunogen Recombinant full length Insulin expressed in Yeast.
Clone HPI-B5
Isotype IgG1

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Insulin (INS Antibody Abstract)
Background Proinsulin is a prohormone precursor to Insulin, made by the pancreas in response to increased blood sugar. Proinsulin is 39 amino acids longer than the mature Insulin, of which 4 are completely lost and the remaining 35 forms the C-peptide. The measurement of Proinsulin in serum provides a useful, valuable information for the diagnosis of insulinomas. Proinsulin levels have also been shown to be elevated in non-insulin dependent diabetics (NIDDM), in insulin dependent diabetics (IDDM) and other clinical situations.Synonyms: INS
Gene ID 3630
UniProt P01308
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling Images back to top
Application Notes Indirect ELISA: Use at 2 μg/mL (For detection of antigen). Western Blot: Use at 4-5 μg/mL. Immunohistochemistry: Use at 20-40 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details Images back to top
Concentration 0.5 mg/mL
Buffer PBS containing containing 30 % Glycerol, 0.5 % BSA and 0.01 % Thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °CAvoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Insulin (INS) antibody (ABIN487416) anti-Insulin (INS) antibody
Western Blotting (WB) image for anti-Insulin (INS) antibody (ABIN487416) anti-Insulin (INS) antibody (Image 2)