anti-Hamster Insulin antibody for Immunohistochemistry (Frozen Sections)

Recommended Insulin Antibody

Antigen
Insulin (INS) Antibodies
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • INS-IGF2
  • Ins2
Reactivity
Hamster, Human, Pig (Porcine), Rat (Rattus)
717
274
246
220
197
108
9
8
6
6
6
4
2
2
2
2
2
2
2
1
1
1
1
Host
Guinea Pig
539
213
49
4
2
1
Clonality
Polyclonal
Conjugate
This Insulin antibody is un-conjugated
39
29
24
15
13
13
8
8
8
8
8
8
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
2
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
335
227
192
165
158
127
107
66
56
33
30
30
23
21
21
7
5
4
4
4
3
2
2
2
1
1
1
1
1
Options
Catalog No. ABIN115757
$ 665.50
Plus shipping costs $45.00

General

Antigen Insulin (INS) Antibodies
Reactivity Hamster, Human, Pig (Porcine), Rat (Rattus)
(717), (274), (246), (220), (197), (108), (9), (8), (6), (6), (6), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Host Guinea Pig
(539), (213), (49), (4), (2), (1)
Clonality
Polyclonal
Conjugate This Insulin antibody is un-conjugated
(39), (29), (24), (15), (13), (13), (8), (8), (8), (8), (8), (8), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(335), (227), (192), (165), (158), (127), (107), (66), (56), (33), (30), (30), (23), (21), (21), (7), (5), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 4 references available
Supplier

Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling References for anti-Insulin antibody (ABIN115757) Images
Specificity The antibody recognizes Insulin produced by the B-cells of the pancreatic islets and by insulin-producing islet cell tumors. Absorption with 10-100 μg proinsulin per ml diluted antiserum inactivates the antiserum, while C-peptide and Insulin only partly reduce staining.
Cross-Reactivity (Details) Also reacts to: Proinsulin
Purification Serum
Immunogen Synthetic Human proinsulin
Plasmids, Primers & others

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling References for anti-Insulin antibody (ABIN115757) Images back to top
Antigen
Alternative Name Insulin (INS Antibody Abstract)
Background Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.Synonyms: INS
Gene ID 3630
NCBI Accession NP_000198
UniProt P01308
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling References for anti-Insulin antibody (ABIN115757) Images back to top
Application Notes Immunofluorescence: 1: 1000 - 1: 2000, overnight incubation at 2-8C. Immunohistochemistry on frozen and paraffin sections: 1: 3000 - 1: 5000. Positive Control: Formalin-fixed paraffin sections of human pancreas
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details References for anti-Insulin antibody (ABIN115757) Images back to top
Format Liquid
Reconstitution Dissolve the antiserum in 50-100 μL distilled water, and dilute further in 0.1 M PBS with 1 % BSA and 0.09 % Sodium Azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody (in aliquots) at -20 °C.

References for anti-Insulin antibody (ABIN115757)

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling Images back to top
Background publications

Kohnert, Axcrona, Hehmke, Klöting, Sundler, Ahrén: "Islet neuronal abnormalities associated with impaired insulin secretion in type 2 diabetes in the Chinese hamster." in: Regulatory peptides, Vol. 82, Issue 1-3, pp. 71-9, 1999

Ahrén, Gudbjartsson, Al-Amin, Mårtensson, Myrsén-Axcrona, Karlsson, Mulder, Sundler: "Islet perturbations in rats fed a high-fat diet." in: Pancreas, Vol. 18, Issue 1, pp. 75-83, 1999

Myrsén-Axcrona, Karlsson, Sundler, Ahrén: "Dexamethasone induces neuropeptide Y (NPY) expression and impairs insulin release in the insulin-producing cell line RINm5F. Release of NPY and insulin through different pathways." in: The Journal of biological chemistry, Vol. 272, Issue 16, pp. 10790-6, 1997

Myrsén, Ahrén, Sundler: "Dexamethasone-induced neuropeptide Y expression in rat islet endocrine cells. Rapid reversibility and partial prevention by insulin." in: Diabetes, Vol. 45, Issue 10, pp. 1306-16, 1996