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|Antigen||Insulin (INS) Antibodies|
|Conjugate||This Insulin antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|1 reference available|
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Product Details anti-Insulin AntibodyTarget Details Insulin Application Details Handling References for anti-Insulin antibody (ABIN111474) Images
|Specificity||This monoclonal C-PEP-01 anitbody reacts with the C-peptide of Human Proinsulin. In normal tissues it reacts with normal pancreatic islet Beta-cells. In tumor tissues it stains Insulin secreting neoplasms (insulinomas). The antibody reacts with Human Proinsulin but not with Insulin, Glucagon or Somatostatin, and recognizes the amino acid residues 8-13 and 25-31.|
Species reactivity (tested):Human.
Also reacts to: Proinsulin
|Immunogen||C-Peptide of Human Proinsulin|
Target Details InsulinProduct Details anti-Insulin Antibody Application Details Handling References for anti-Insulin antibody (ABIN111474) Images back to top
|Alternative Name||Insulin (INS Antibody Abstract)|
|Background||A precursor of Insulin it is converted to Insulin by removal of the connecting C peptide, leaving the two (A and B) chains. From every molecule of Proinsulin, one molecule of Insulin plus one molecule of C Peptide are produced. C Peptide is part of the molecule of Proinsulin, that consists of three parts: C Peptide and two long strands of amino acids (called the alpha and beta chains) that later become linked together to form the insulin molecule. From every molecule of proinsulin, one molecule of insulin plus one molecule of C Peptide are produced. C peptide is released into the blood stream in equal amounts to insulin. A test of C peptide levels will show how much insulin the body is making. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.Synonyms: INS|
|Research Area||Neurology, Diabetes, Hormones|
|Pathways||NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation|
Application DetailsProduct Details anti-Insulin Antibody Target Details Insulin Handling References for anti-Insulin antibody (ABIN111474) Images back to top
Immunohistochemistry on Paraffin Sections: 0.2 μg/mL (1/5000) (No pretreatmentnecessary). Recommended Positive Control: Human pancreas. Has been described to work in ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Protocol||Protocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum|
|Restrictions||For Research Use only|
HandlingProduct Details anti-Insulin Antibody Target Details Insulin Application Details References for anti-Insulin antibody (ABIN111474) Images back to top
|Reconstitution||Restore with 0.5 mL distilled water.|
|Buffer||Stock solution contains PBS, pH 7.2 with 5 mg/mL BSA as a stabilizer and 0.1 % Kathon as a preservative|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-Insulin antibody (ABIN111474)Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling Images back to top
Hilgert, Stolba, Kristofová, Stefanová, Bendlová, Lebl, Horejsí: "A monoclonal antibody applicable for determination of C-peptide of human proinsulin by RIA." in: Hybridoma, Vol. 10, Issue 3, pp. 379-86, 1991