anti-Pig (Porcine) Insulin antibody for Enzyme Immunoassay

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • INS-IGF2
  • Ins2
Reactivity
Human, Pig (Porcine)
952
468
370
350
240
194
45
11
9
8
7
7
6
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Host
Mouse
785
197
49
9
4
2
1
Clonality
Monoclonal
Conjugate
This Insulin antibody is un-conjugated
52
41
36
21
20
20
20
15
15
15
13
13
13
13
13
13
13
10
9
9
7
7
7
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
2
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
461
358
276
269
259
236
180
118
106
66
66
31
25
23
21
8
7
5
4
4
4
3
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN5556681
$ 619.30
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN316680 EIA IHC (p) Mouse IgG1 Log in to see 2D11 0
1 ABIN112214 EIA IHC (fro) IHC (p) Mouse IgG1 Log in to see D3E7 (5B6-6) 1
1 ABIN487416 EIA IHC (fro) WB Mouse IgG1 Log in to see HPI-B5 0
1 ABIN272321 EIA IHC (p) WB Mouse IgG1 Log in to see E2E3 3
1 ABIN111965 EIA Func IHC (p) RIA Mouse IgG1 Log in to see IN-05 1
1 ABIN112204 EIA IHC (fro) Mouse IgG1 Log in to see 5E4-3 0
1 ABIN1107764 EIA IHC (p) Mouse IgG1 Log in to see E2-E3 0
1 ABIN112182 ELISA (Capture) EIA Mouse IgG1 Log in to see E6-E5 (7F8) 0
1 ABIN111963 EIA IHC (fro) IHC (p) Mouse IgG1 Log in to see IN-05 1
1 ABIN113504 EIA IHC (fro) IHC (p) Rabbit Log in to see Polyclonal 1
1 ABIN5540065 DB EIA ICC ID IF WB Guinea Pig IgG Log in to see Polyclonal 0

General

Antigen Insulin (INS) Antibodies
Reactivity Human, Pig (Porcine)
(952), (468), (370), (350), (240), (194), (45), (11), (9), (8), (7), (7), (6), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(785), (197), (49), (9), (4), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Insulin antibody is un-conjugated
(52), (41), (36), (21), (20), (20), (20), (15), (15), (15), (13), (13), (13), (13), (13), (13), (13), (10), (9), (9), (7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(461), (358), (276), (269), (259), (236), (180), (118), (106), (66), (66), (31), (25), (23), (21), (8), (7), (5), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling Images
Sequence Human Proinsulin (precursor) 21 aa A chain 90-110. 30 aa B chain 25-54. 1 MALWMRLLPL LALLALWGPD PAAAFVNQHL CGSHLVEALY LVCGERGFFY TPKTRREAED 61 LQVGQVELGG GPGAGSLQPL ALEGSLQKRG IVEQCCTSIC SLYQLENYCN
Specificity This monoclonal 2D11.H5 antibody recognizes a polypeptide which is identified as Insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. This protein A purified Mouse monoclonal antibody reacts specifically with Insulin from Human and Swine sources. Cross reactivity with Insulin from Mouse and Rat does not occur. Cross reactivity with Insulin from other sources has not been determined.
Cellular Localization: Cytoplasmic.Cellular Localization:
Characteristics Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)
Purification Protein A Chromatography
Immunogen Purified human Insulin coupled to Bovine Serum Albumin (BSA).
Clone 2D11-H5
Isotype IgG1
Plasmids, Primers & others

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Insulin (INS Antibody Abstract)
Background Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-peptide basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain acids. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as β-cell and tumor (insulinoma) markers.Synonyms: INS
Molecular Weight 6 kDa
Gene ID 3630
NCBI Accession NP_000198
UniProt P01308
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling Images back to top
Application Notes This antibody is suitable for ELISA, Immunohistochemistry, Immunoblotting andimmunoprecipitation. Insulin has a cytoplasmic localization. Pancreatic tissue or β-cellsfrom islets of Langerhans can be used as a positive control. Generally a 1: 50 to 1: 200 dilution is suitable for most applications. A 30 to 60 minuteincubation time is usually sufficient. However, depending upon the fixation conditions andthe staining system employed, optimal incubation should be determined by the user. Frozen sections of paraffin embedded tissues are recommended for preparations to use forimmunohistochemistry. No special pretreatment is required. Researchers shoulddetermine the optimal dilution for other specific assays and cell types. ELISA: Results of Mab anti-Insulin antibody were tested against human insulin by ELISA. Each well was coated with 0.1g of conjugate. The starting concentration of antibody in thedilution series was 10 μg/mL. Each point on the Y-axis represents a 3-fold dilution. Themidpoint of the titration curve represents approx-imately 5 ng/mL antibody or a 1: 200,000dilution from the stock concentration. HRP conjugated Gt-a- Mouse IgG H&L and TMBsubstrate were used for detection (see figure 1).
Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details Images back to top
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Storage -20 °C
Storage Comment Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.