anti-Pig (Porcine) Insulin antibody for Immunodiffusion

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • INS-IGF2
  • Ins2
Reactivity
Pig (Porcine)
877
391
263
258
207
120
49
6
6
6
5
4
4
2
2
1
1
1
1
1
1
1
Host
Guinea Pig
709
179
52
16
3
1
Clonality
Polyclonal
Conjugate
This Insulin antibody is un-conjugated
47
38
37
23
22
22
22
17
17
14
14
14
14
14
14
14
14
10
9
8
8
8
5
5
5
5
5
5
2
1
Application
Dot Blot (DB), Enzyme Immunoassay (EIA), Immunocytochemistry (ICC), Immunodiffusion (ID), Immunofluorescence (IF), Western Blotting (WB)
507
353
228
205
190
181
146
123
66
64
33
25
15
11
8
6
5
4
4
3
2
2
2
1
1
1
1
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Catalog No. ABIN5540065
$ 569.25
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General

Antigen Insulin (INS) Antibodies
Reactivity Pig (Porcine)
(877), (391), (263), (258), (207), (120), (49), (6), (6), (6), (5), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Guinea Pig
(709), (179), (52), (16), (3), (1)
Clonality
Polyclonal
Conjugate This Insulin antibody is un-conjugated
(47), (38), (37), (23), (22), (22), (22), (17), (17), (14), (14), (14), (14), (14), (14), (14), (14), (10), (9), (8), (8), (8), (5), (5), (5), (5), (5), (5), (2), (1)
Application Dot Blot (DB), Enzyme Immunoassay (EIA), Immunocytochemistry (ICC), Immunodiffusion (ID), Immunofluorescence (IF), Western Blotting (WB)
(507), (353), (228), (205), (190), (181), (146), (123), (66), (64), (33), (25), (15), (11), (8), (6), (5), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1)
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling Images
Specificity The reactivity of the antiserum is directed to Insulin as tested by ELISA and Immunoblotting. No reaction was obtained with other Swine serum proteins.
Purification Hyperimmune antisera with strong precipitating activity are selected for fractionation by Salt Precipitation and purification of the IgG fraction by DEAE Chromatography
Immunogen Insulin hormone is isolated from Porcine pancreas. Freund's complete adjuvant is used in the first step of the immunization procedure.
Isotype IgG

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling Images back to top
Antigen
Alternative Name insulin (INS Antibody Abstract)
Background Insulin is a hormone produced by the beta cells of the islets of Langerhans in the pancreas. It plays an important role in the metabolism of carbohydrate by its effects on the storage of glycogen in the liver, the conversion of carbohydrate to fat, and the oxidation of glucose by the peripheral tissues. Insulin has a molecular weight of 6,000 and contains two peptide chains linked by two disulphide bonds.
UniProt P01315
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling Images back to top
Application Notes The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates in radioimmunoassay for the production of immunoconjugates with a selected marker to prepare immunoaffinity adsorbents by coupling to an artificial carrier in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent in Western blotting. This product is not pre-diluted. Recommended Working Dilutions : Histochemical and Cytochemical Use: are usually between 1/100-1/250. In ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000.
Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details Images back to top
Buffer PBS, pH 7.2 Preservatives: None, as it may interfere with the antibody activity. No foreign proteins added
Preservative Azide free
Storage 4 °C,-20 °C
Storage Comment Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months