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|Antigen||Insulin (INS) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Insulin antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|3 references available|
|Supplier||Log in to see|
Product Details anti-Insulin AntibodyTarget Details Insulin Application Details Handling References for anti-Insulin antibody (ABIN372838) Images
|Specificity||This antibody recognizes endogenous levels of total Human Insulin protein.|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Immunogen||INS antibody was raised against Synthetic peptide (KLH-coupled) derived from the sequence of Human Insulin.|
Target Details InsulinProduct Details anti-Insulin Antibody Application Details Handling References for anti-Insulin antibody (ABIN372838) Images back to top
|Alternative Name||Insulin (INS Antibody Abstract)|
|Background||Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.Synonyms: INS|
|Pathways||NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation|
Application DetailsProduct Details anti-Insulin Antibody Target Details Insulin Handling References for anti-Insulin antibody (ABIN372838) Images back to top
ELISA. Flow Cytometry. Immunofluorescence. Immunocytochemistry. Immunohistochemistry on Paraffin Sections: 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Insulin Antibody Target Details Insulin Application Details References for anti-Insulin antibody (ABIN372838) Images back to top
|Buffer||10 mM HEPES, pH 7.5, 150 mM Sodium Chloride, 100 μg/mL BSA, 50 % Glycerol.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-Insulin antibody (ABIN372838)Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling Images back to top
|Product cited in:||
Micucci, Orciari, Catalano: "Hyperglycemia promotes K-Ras-induced lung tumorigenesis through BASCs amplification." in: PLoS ONE, Vol. 9, Issue 8, pp. e105550, 2014
Madsen, Knauf, Gotfredsen, Pilling, Sjögren, Andersen, Andersen, de Boer, Manova, Barlas, Vundavalli, Nyborg, Knudsen, Moelck, Fagin: "GLP-1 receptor agonists and the thyroid: C-cell effects in mice are mediated via the GLP-1 receptor and not associated with RET activation." in: Endocrinology, Vol. 153, Issue 3, pp. 1538-47, 2012
"Retraction." in: Endocrinology, 2010
ImagesProduct Details anti-Insulin Antibody Target Details Insulin Application Details Handling References for anti-Insulin antibody (ABIN372838) back to top