anti-Rat (Rattus) Insulin antibody for Flow Cytometry

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • INS-IGF2
  • Ins2
Reactivity
Human, Mouse (Murine), Rat (Rattus)
951
470
369
350
239
194
45
11
9
8
7
7
6
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Host
Rabbit
786
196
50
9
4
2
1
Clonality
Polyclonal
Conjugate
This Insulin antibody is un-conjugated
52
41
36
21
20
20
20
15
15
15
13
13
13
13
13
13
13
10
9
9
7
7
7
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
2
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
462
357
275
270
258
238
181
118
106
67
66
31
25
23
21
8
7
6
5
4
4
3
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN372838
$ 456.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN337249 FACS ICC IF IHC IHC (p) Rabbit IgG Log in to see Polyclonal 0
1 ABIN6157443 FACS IF IHC (f) CF®405S Mouse IgG1, kappa Log in to see IRDN-794 0
1 ABIN6157445 FACS IF IHC (f) CF405M Mouse IgG1, kappa Log in to see IRDN-794 0
1 ABIN6157455 FACS IF IHC (f) CF®660R Mouse IgG1, kappa Log in to see IRDN-794 0
1 ABIN6157453 FACS IF IHC (f) CF®555 Mouse IgG1, kappa Log in to see IRDN-794 0
1 ABIN6157461 FACS IF IHC (f) CF®680 Mouse IgG1, kappa Log in to see IRDN-794 0
1 ABIN6157449 FACS IF IHC (f) CF®543 Mouse IgG1, kappa Log in to see IRDN-794 0
1 ABIN6157465 FACS IF IHC (f) CF®488A Mouse IgG1, kappa Log in to see IRDN-794 0
1 ABIN6157467 FACS IF IHC (f) CF®594 Mouse IgG1, kappa Log in to see IRDN-794 0
1 ABIN6157463 FACS IF IHC (f) CF®680R Mouse IgG1, kappa Log in to see IRDN-794 0
1 ABIN6157447 FACS IF IHC (f) CF®640R Mouse IgG1, kappa Log in to see IRDN-794 0
1 ABIN6157451 FACS IF IHC (f) CF®647 Mouse IgG1, kappa Log in to see IRDN-794 0
1 ABIN6157459 FACS IF IHC (f) CF®770 Mouse IgG1, kappa Log in to see IRDN-794 0
1 ABIN6157457 FACS IF IHC (f) CF®568 Mouse IgG1, kappa Log in to see IRDN-794 0
1 ABIN6157478 FACS IF IHC (f) Mouse IgG1, kappa Log in to see IRDN-794 0
1 ABIN6157470 FACS IF IHC (f) Alkaline Phosphatase (AP) Mouse IgG1, kappa Log in to see IRDN-794 0
1 ABIN6157472 FACS IF IHC (f) Biotin Mouse IgG1, kappa Log in to see IRDN-794 0
1 ABIN6157477 FACS IF IHC (f) PE Mouse IgG1, kappa Log in to see IRDN-794 0
1 ABIN6157476 FACS IF IHC (f) PerCP Mouse IgG1, kappa Log in to see IRDN-794 0
1 ABIN6157469 FACS IF IHC (f) APC Mouse IgG1, kappa Log in to see IRDN-794 0

General

Antigen Insulin (INS) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(951), (470), (369), (350), (239), (194), (45), (11), (9), (8), (7), (7), (6), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(786), (196), (50), (9), (4), (2), (1)
Clonality
Polyclonal
Conjugate This Insulin antibody is un-conjugated
(52), (41), (36), (21), (20), (20), (20), (15), (15), (15), (13), (13), (13), (13), (13), (13), (13), (10), (9), (9), (7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(462), (357), (275), (270), (258), (238), (181), (118), (106), (67), (66), (31), (25), (23), (21), (8), (7), (6), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling References for anti-Insulin antibody (ABIN372838) Images
Specificity This antibody recognizes endogenous levels of total Human Insulin protein.
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Immunoaffinity Chromatography.
Immunogen INS antibody was raised against Synthetic peptide (KLH-coupled) derived from the sequence of Human Insulin.
Isotype IgG
Plasmids, Primers & others

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling References for anti-Insulin antibody (ABIN372838) Images back to top
Antigen
Alternative Name Insulin (INS Antibody Abstract)
Background Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.Synonyms: INS
Gene ID 3630
NCBI Accession NP_000198
UniProt P01308
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling References for anti-Insulin antibody (ABIN372838) Images back to top
Application Notes ELISA. Flow Cytometry. Immunofluorescence. Immunocytochemistry. Immunohistochemistry on Paraffin Sections: 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details References for anti-Insulin antibody (ABIN372838) Images back to top
Format Liquid
Concentration 0.02 mg/mL
Buffer 10 mM HEPES, pH 7.5, 150 mM Sodium Chloride, 100 μg/mL BSA, 50 % Glycerol.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-Insulin antibody (ABIN372838)

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling Images back to top
Product cited in:

Micucci, Orciari, Catalano: "Hyperglycemia promotes K-Ras-induced lung tumorigenesis through BASCs amplification." in: PLoS ONE, Vol. 9, Issue 8, pp. e105550, 2014

Madsen, Knauf, Gotfredsen, Pilling, Sjögren, Andersen, Andersen, de Boer, Manova, Barlas, Vundavalli, Nyborg, Knudsen, Moelck, Fagin: "GLP-1 receptor agonists and the thyroid: C-cell effects in mice are mediated via the GLP-1 receptor and not associated with RET activation." in: Endocrinology, Vol. 153, Issue 3, pp. 1538-47, 2012

"Retraction." in: Endocrinology, 2010

Images

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling References for anti-Insulin antibody (ABIN372838) back to top
Supplier Images
 image for anti-Insulin (INS) antibody (ABIN372838) anti-Insulin (INS) antibody