anti-Rat (Rattus) Insulin antibody for Flow Cytometry

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • INS-IGF2
  • Ins2
Reactivity
Human, Mouse (Murine), Rat (Rattus)
876
392
262
258
206
120
49
6
6
6
5
4
4
2
2
1
1
1
1
1
1
1
Host
Rabbit
709
178
53
16
3
1
Clonality
Polyclonal
Conjugate
This Insulin antibody is un-conjugated
47
38
37
23
22
22
22
17
17
14
14
14
14
14
14
14
14
10
9
8
8
8
5
5
5
5
5
5
2
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
507
352
229
205
190
180
146
124
66
64
33
25
15
11
8
6
5
4
4
3
3
2
2
1
1
1
1
1
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Catalog No. ABIN372838
$ 456.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN337249 FACS ICC IF IHC (p) Rabbit IgG Log in to see Polyclonal 0
1 ABIN4326141 FACS IF IHC (p) Mouse IgG1 kappa Log in to see IRDN-794 0
1 ABIN4326142 FACS IF IHC (p) Mouse IgG1 kappa Log in to see IRDN-794 0
1 ABIN4326143 FACS IF IHC (p) Mouse IgG1 kappa Log in to see IRDN-794 0
1 ABIN1735946 ICC FACS IF IHC (p) ELISA Rabbit IgG Log in to see Polyclonal 0
1 ABIN4326122 FACS APC Mouse IgG1 kappa Log in to see SPM531 0
1 ABIN4326147 CyTOF FACS IF IHC (p) Mouse IgG1 kappa Log in to see IRDN-794 0
1 ABIN4326148 CyTOF FACS IF IHC (p) Mouse IgG1 kappa Log in to see IRDN-794 0
1 ABIN2742157 FACS IF IHC (p) IP WB Mouse IgG1, kappa AA 1-84 Log in to see E2-E3-2D11-H5 or INS04-INS05 0
1 ABIN2742156 ELISA FACS IF IHC (p) IP WB Mouse IgG1, kappa AA 1-84 Log in to see E2-E3 or INS04 0
1 ABIN4326155 FACS IHC (p) Alexa Fluor 488 Mouse IgG1 kappa Log in to see IRDN-794 0
1 ABIN4326154 FACS IHC (p) Alexa Fluor 405 Mouse IgG1 kappa Log in to see IRDN-794 0
1 ABIN4326158 FACS APC Mouse IgG1 kappa Log in to see IRDN-794 0
1 ABIN4326161 FACS IHC (p) FITC Mouse IgG1 kappa Log in to see IRDN-794 0
1 ABIN4326163 FACS IHC (p) DyLight 488 Mouse IgG1 kappa Log in to see IRDN-794 0
1 ABIN4326165 FACS IHC (p) DyLight 755 Mouse IgG1 kappa Log in to see IRDN-794 0
1 ABIN4326170 FACS IHC (p) DyLight 405 Mouse IgG1 kappa Log in to see IRDN-794 0
1 ABIN4326167 FACS PE Mouse IgG1 kappa Log in to see IRDN-794 0
1 ABIN4326156 FACS IHC (p) Alexa Fluor 647 Mouse IgG1 kappa Log in to see IRDN-794 0
1 ABIN4326157 FACS IHC (p) Alexa Fluor 700 Mouse IgG1 kappa Log in to see IRDN-794 0

General

Antigen Insulin (INS) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(876), (392), (262), (258), (206), (120), (49), (6), (6), (6), (5), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(709), (178), (53), (16), (3), (1)
Clonality
Polyclonal
Conjugate This Insulin antibody is un-conjugated
(47), (38), (37), (23), (22), (22), (22), (17), (17), (14), (14), (14), (14), (14), (14), (14), (14), (10), (9), (8), (8), (8), (5), (5), (5), (5), (5), (5), (2), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(507), (352), (229), (205), (190), (180), (146), (124), (66), (64), (33), (25), (15), (11), (8), (6), (5), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling References for anti-Insulin antibody (ABIN372838) Images
Specificity This antibody recognizes endogenous levels of total Human Insulin protein.
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Immunoaffinity Chromatography.
Immunogen INS antibody was raised against Synthetic peptide (KLH-coupled) derived from the sequence of Human Insulin.
Isotype IgG

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling References for anti-Insulin antibody (ABIN372838) Images back to top
Antigen
Alternative Name Insulin (INS Antibody Abstract)
Background Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.Synonyms: INS
Gene ID 3630
NCBI Accession NP_000198
UniProt P01308
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling References for anti-Insulin antibody (ABIN372838) Images back to top
Application Notes ELISA. Flow Cytometry. Immunofluorescence. Immunocytochemistry. Immunohistochemistry on Paraffin Sections: 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details References for anti-Insulin antibody (ABIN372838) Images back to top
Format Liquid
Concentration 0.02 mg/mL
Buffer 10 mM HEPES, pH 7.5, 150 mM Sodium Chloride, 100 μg/mL BSA, 50 % Glycerol.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-Insulin antibody (ABIN372838)

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling Images back to top
Product cited in:

Micucci, Orciari, Catalano: "Hyperglycemia promotes K-Ras-induced lung tumorigenesis through BASCs amplification." in: PLoS ONE, Vol. 9, Issue 8, pp. e105550, 2014

Madsen, Knauf, Gotfredsen, Pilling, Sjögren, Andersen, Andersen, de Boer, Manova, Barlas, Vundavalli, Nyborg, Knudsen, Moelck, Fagin: "GLP-1 receptor agonists and the thyroid: C-cell effects in mice are mediated via the GLP-1 receptor and not associated with RET activation." in: Endocrinology, Vol. 153, Issue 3, pp. 1538-47, 2012

"Retraction." in: Endocrinology, 2010

Images

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling References for anti-Insulin antibody (ABIN372838) back to top
Supplier Images
 image for anti-Insulin (INS) antibody (ABIN372838) anti-Insulin (INS) antibody