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|Antigen||Insulin Receptor (INSR) Antibodies|
|Epitope||Beta Chain Alternatives|
|Reactivity||Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Insulin Receptor antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
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Product Details anti-Insulin Receptor AntibodyTarget Details Insulin Receptor Application Details Handling Images
|Specificity||This antibody specifically recognizes the C-terminus of Insulin receptor (phosphorylation-independent).|
|Characteristics||Synonyms: Insulin Receptor|
|Purification||Subsequent thiophilic adsorption and size exclusion chromatography|
|Components||incl. positive Control|
|Immunogen||Synthetic peptide conjugated to KLH|
Target Details Insulin ReceptorProduct Details anti-Insulin Receptor Antibody Application Details Handling Images back to top
|Alternative Name||CD220 / INSR (INSR Antibody Abstract)|
|Background||The insulin receptor (InsR) is a heterodimeric receptor tyrosine kinase with an extracellular alpha-chain, a transmembrane domain and an intracellular beta-chain. The insulin receptor is activated upon binding of the peptide hormone insulin, leading to autophosphorylation of tyrosine residues 1146, 1150, and 1151 in the activation loop of the beta-chain. Additional phosphorylation sites such as tyrosine residues 960, 1316, and 1322 regulate the assembly of signal transduction complexes.Synonyms: Insulin Receptor|
|Pathways||NF-kappaB Signaling, RTK Signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity|
Application DetailsProduct Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Handling Images back to top
Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from untreated HepG2 cells. ELISA: 0.1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Protocol||Positive Control Cell LysateFormulation: Lyophilized cell lysate from serum starved HepG2 cells. Recostitution: Reconstitute by addition of 200 µl H2O. After complete solubilization add200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90 °C for 5 min. Aliquote and store frozen. Avoid repeated freezing and thawing. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80. 000 cells. Use 20µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplications with native proteins such as immunoprecipitation.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Application Details Images back to top
|Reconstitution||Restore with 1 mL H2O (15 min, RT).|
|Buffer||1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -20 to -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
Shelf life: one year from despatch.
|Expiry Date||12 months|
ImagesProduct Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Application Details Handling back to top