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|Antigen||Nerve Growth Factor beta (NGFB) Antibodies|
|Conjugate||This NGFB antibody is un-conjugated Alternatives|
Immunohistochemistry (IHC), Neutralization (Neut), ELISA, Western Blotting (WB)
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Product Details anti-NGFB AntibodyTarget Details NGFB Application Details Handling Images
E.coli derived recombinant Human beta-NGF.
Type of Immunogen: Cell extract
Target Details NGFBProduct Details anti-NGFB Antibody Application Details Handling Images back to top
|Alternative Name||NGF (NGFB Antibody Abstract)|
Name/Gene ID: NGF
Family: NGF Beta
Synonyms: NGF, Beta-nerve growth factor, ProNGF, Beta-NGF, HSAN5, NGFB
|Pathways||NF-kappaB Signaling, RTK Signaling, Regulation of Cell Size|
Application DetailsProduct Details anti-NGFB Antibody Target Details NGFB Handling Images back to top
Approved: ELISA (0.5 - 2 μg/mL), IHC, Neut (5 - 8 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human pancreas adenocarcinoma. The recommended concentration is 0.5 mg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results were achieved without antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human beta-NGF (1 ng/mL), a concentration of 0.05-0.08 μg/mL of this antibody is required. ELISA: To detect Human beta-NGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human beta-NGF (LS-C104735) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human beta-NGF. Western Blot: To detect Human beta-NGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human beta-NGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
|Restrictions||For Research Use only|
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|Buffer||Lyophilized from PBS, pH 7.2|
|Handling Advice||Avoid freeze/thaw cycles.|
|Storage||4 °C,-20 °C|
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
ImagesProduct Details anti-NGFB Antibody Target Details NGFB Application Details Handling back to top