anti-Human SQSTM1 antibody for Immunoprecipitation

Recommended SQSTM1 Antibody (supplied by: Log in to see )

Antigen
Sequestosome 1 (SQSTM1) Antibodies
  • SQSTM1
  • sqstm1
  • MGC79491
  • A170
  • OSIL
  • PDB3
  • ZIP3
  • p60
  • p62
  • p62B
  • OSF-6
  • Osi
  • STAP
  • ZIP
  • sb:cb621
  • zgc:85784
  • sequestosome 1
  • sequestosome 1 L homeolog
  • SQSTM1
  • sqstm1
  • Sqstm1
  • sqstm1.L
Epitope
Internal Region
68
41
36
36
24
19
19
16
15
14
12
12
11
9
8
8
8
6
5
5
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Bee (Apiformes), Human, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)
377
198
126
8
5
5
5
4
4
4
2
2
2
2
1
1
1
1
Host
Rabbit
299
69
6
3
Clonality
Polyclonal
Conjugate
This SQSTM1 antibody is un-conjugated
20
18
17
13
13
12
6
6
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
309
131
115
91
81
69
50
38
35
28
17
13
4
3
2
Options
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Catalog No. ABIN2854836
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
23.915068 ABIN5970256 FACS IF/ICC IHC IP WB Rabbit IgG Log in to see Polyclonal 0
23.915068 ABIN5998960 IHC IP WB Rabbit IgG Log in to see Polyclonal 0
23.915068 ABIN6148418 IHC IP WB Rabbit Log in to see Polyclonal 0
1 ABIN4343150 ICC IF IHC IHC (p) IP WB Rabbit AA 425-440 Log in to see Polyclonal 3
1 ABIN1874934 FACS IF IHC IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN4343148 IHC IHC (p) IP SimWes WB Rabbit AA 325-375 Log in to see Polyclonal 2
1 ABIN4343149 ICC IF IHC IHC (p) IP WB Rabbit IgG AA 375-425 Log in to see Polyclonal 0
1 ABIN1920949 IF IHC IHC (p) IP WB Rabbit IgG AA 375-425 Log in to see Polyclonal 0
1 ABIN1920950 IF IHC IHC (p) IP WB Rabbit IgG AA 425-440 Log in to see Polyclonal 0
1 ABIN5964003 IHC IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2701519 IF IP IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2910821 IF/ICC IHC IP WB Rabbit AA 136-400 Log in to see Polyclonal 0
1 ABIN5875316 ELISA FACS IF/ICC IHC IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6027170 IF/ICC IHC IP WB Mouse Log in to see 0
1 ABIN5557948 ELISA IHC IP WB FITC Rabbit Log in to see Polyclonal 0
1 ABIN5557947 ELISA IHC IP WB Biotin Rabbit Log in to see Polyclonal 0
1 ABIN5557946 ELISA IHC IP WB Rabbit Log in to see Polyclonal 0

General

Antigen Sequestosome 1 (SQSTM1) Antibodies
Epitope Internal Region
(68), (41), (36), (36), (24), (19), (19), (16), (15), (14), (12), (12), (11), (9), (8), (8), (8), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Bee (Apiformes), Human, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)
(377), (198), (126), (8), (5), (5), (5), (4), (4), (4), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(299), (69), (6), (3)
Clonality
Polyclonal
Conjugate This SQSTM1 antibody is un-conjugated
(20), (18), (17), (13), (13), (12), (6), (6), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(309), (131), (115), (91), (81), (69), (50), (38), (35), (28), (17), (13), (4), (3), (2)
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Product Details anti-SQSTM1 Antibody

Target Details SQSTM1 Application Details Handling Images
Cross-Reactivity Rhesus Monkey
Cross-Reactivity (Details) Rhesus Monkey (93 %)
Characteristics Rabbit Polyclonal antibody to SQSTM1 (sequestosome 1)
SQSTM1 antibody [N3C1], Internal
Purification Purified by antigen-affinity chromatography.
Immunogen Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human SQSTM1. The exact sequence is proprietary.
Isotype IgG
Plasmids, Primers & others

Target Details SQSTM1

Product Details anti-SQSTM1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name SQSTM1 (SQSTM1 Antibody Abstract)
Background This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

Cellular Localization: Cytoplasm , Late endosome , Nucleus
Molecular Weight 48 kDa
Gene ID 8878
Pathways NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy

Application Details

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Handling Images back to top
Application Notes Suggested dilution Reference Flow cytometry/FACS 1:50-1:200* ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceFlow cytometry/FACS1:50-1:200* ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
Comment

Positive Control: A549 , NIH-3T3 , JC , BCL-1 , PC-12 , Rat2 , Huh7 (untreated) , Huh7 ( 3uM Thapsigargin treatment for 12hr) , *HeLa

Restrictions For Research Use only

Handling

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 1XPBS, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Handling back to top
Supplier Images
Immunoprecipitation (IP) image for anti-Sequestosome 1 (SQSTM1) (Internal Region) antibody (ABIN2854836) IP Image Immunoprecipitation of SQSTM1 protein from HeLa whole cell extracts using 5 ...
Immunofluorescence (IF) image for anti-Sequestosome 1 (SQSTM1) (Internal Region) antibody (ABIN2854836) ICC/IF Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome...
Western Blotting (WB) image for anti-Sequestosome 1 (SQSTM1) (Internal Region) antibody (ABIN2854836) WB Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by western blot anal...
Immunohistochemistry (IHC) image for anti-Sequestosome 1 (SQSTM1) (Internal Region) antibody (ABIN2854836) IHC-P Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at cytoplasm on h...
Western Blotting (WB) image for anti-Sequestosome 1 (SQSTM1) (Internal Region) antibody (ABIN2854836) WB Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by Western blot anal...
Western Blotting (WB) image for anti-Sequestosome 1 (SQSTM1) (Internal Region) antibody (ABIN2854836) WB Image SQSTM1 antibody detects SQSTM1 protein by western blot analysis. Whole cell ...
Western Blotting (WB) image for anti-Sequestosome 1 (SQSTM1) (Internal Region) antibody (ABIN2854836) WB Image Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE antibody diluted at...
Immunofluorescence (IF) image for anti-Sequestosome 1 (SQSTM1) (Internal Region) antibody (ABIN2854836) ICC/IF Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome...
Flow Cytometry (FACS) image for anti-Sequestosome 1 (SQSTM1) (Internal Region) antibody (ABIN2854836) FACS Image SQSTM1 antibody [N3C1], Internal , detects SQSTM1 protein by flow cytometr...