anti-Mouse (Murine) SQSTM1 antibody for Immunohistochemistry (Frozen Sections)

Recommended SQSTM1 Antibody (supplied by: Log in to see )

Antigen
Sequestosome 1 (SQSTM1) Antibodies
  • SQSTM1
  • sqstm1
  • MGC79491
  • A170
  • OSIL
  • PDB3
  • ZIP3
  • p60
  • p62
  • p62B
  • OSF-6
  • Osi
  • STAP
  • ZIP
  • sb:cb621
  • zgc:85784
  • sequestosome 1
  • sequestosome 1 L homeolog
  • SQSTM1
  • sqstm1
  • Sqstm1
  • sqstm1.L
Epitope
AA 69-96, N-Term
68
41
36
36
25
20
19
16
15
14
12
12
11
9
9
8
8
6
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
373
190
135
8
5
5
5
5
4
4
2
2
2
2
2
1
1
1
1
Host
Rabbit
314
51
6
3
Clonality
Polyclonal
Conjugate
This SQSTM1 antibody is un-conjugated
19
17
16
12
12
12
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
310
122
102
97
73
70
38
36
28
21
18
13
4
3
2
Options
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Catalog No. ABIN3043937
€ 247.79
Plus shipping costs €45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
15.97553 ABIN4343147 ICC IF IHC IHC (fro) IHC (p) SimWes WB Rabbit AA 300-440 Log in to see Polyclonal 36
1 ABIN334048 ICC IHC IHC (fro) IHC (p) WB Guinea Pig IgG AA 421-440 Log in to see Polyclonal 0
-11.02447 ABIN4343146 ICC IF IHC IHC (fro) IHC (p) SimWes WB Rabbit AA 300-440 Log in to see Polyclonal 0
-11.02447 ABIN4962908 ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Rabbit AA 300-440 Log in to see Polyclonal 0
-11.02447 ABIN4962909 ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Rabbit AA 300-440 Log in to see Polyclonal 0
-11.02447 ABIN4962910 ICC IF IHC (fro) IHC (p) WB Biotin Rabbit AA 300-440 Log in to see Polyclonal 0
-11.02447 ABIN4962906 ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Rabbit AA 300-440 Log in to see Polyclonal 0
-11.02447 ABIN4962912 ICC IF IHC (fro) IHC (p) WB DyLight 488 Rabbit AA 300-440 Log in to see Polyclonal 0
-11.02447 ABIN4962907 ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Rabbit AA 300-440 Log in to see Polyclonal 0
-11.02447 ABIN4962911 ICC IF IHC (fro) IHC (p) WB DyLight 650 Rabbit AA 300-440 Log in to see Polyclonal 0
-11.02447 ABIN4962914 ICC IF IHC (fro) IHC (p) WB DyLight 550 Rabbit AA 300-440 Log in to see Polyclonal 0
-11.02447 ABIN4962913 IHC (fro) IHC (p) WB HRP Rabbit AA 300-440 Log in to see Polyclonal 0

General

Antigen Sequestosome 1 (SQSTM1) Antibodies
Epitope AA 69-96, N-Term
(68), (41), (36), (36), (25), (20), (19), (16), (15), (14), (12), (12), (11), (9), (9), (8), (8), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(373), (190), (135), (8), (5), (5), (5), (5), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(314), (51), (6), (3)
Clonality
Polyclonal
Conjugate This SQSTM1 antibody is un-conjugated
(19), (17), (16), (12), (12), (12), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(310), (122), (102), (97), (73), (70), (38), (36), (28), (21), (18), (13), (4), (3), (2)
Pubmed 5 references available
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Product Details anti-SQSTM1 Antibody

Target Details SQSTM1 Application Details Handling References for anti-SQSTM1 antibody (ABIN3043937) Images
Purpose Rabbit IgG polyclonal antibody for Sequestosome-1(SQSTM1) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
Sequence DEDGDLVAFS SDEELTMAMS YVKDDIFR
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Sequestosome-1(SQSTM1) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
Gene Name: sequestosome 1
Protein Name: Sequestosome-1
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human SQSTM1/p62 (69-96aa DEDGDLVAFSSDEELTMAMSYVKDDIFR), identical to the related mouse and rat sequences.
Isotype IgG
Plasmids, Primers & others

Target Details SQSTM1

Product Details anti-SQSTM1 Antibody Application Details Handling References for anti-SQSTM1 antibody (ABIN3043937) Images back to top
Antigen
Alternative Name SQSTM1 (SQSTM1 Antibody Abstract)
Background SQSTM1(Sequestosome-1), also known as Ubiquitin-Binding Protein P62 or P62, is a protein that in humans is encoded by the SQSTM1 gene. The Src homology type 2 (SH2) domain is a highly conserved motif of about 100 amino acids which mediates protein-protein interactions by binding to phosphotyrosine.p56-lck, a T-cell-specific src family tyrosine kinase with an SH2 domain, is involved in T-cell signal transduction. The International Radiation Hybrid Mapping Consortium mapped the p62 gene to chromosome 5q35. Park et al. (1995) found that the p56-lck SH2 domain binds to p62 at the ser59 of p62 only when that serine is phosphorylated. Joung et al. (1996) expressed epitope-tagged p62 in Hela cells and showed that the expressed protein bound to the lck SH2 domain and that this binding was dependent on the N-terminal 50 amino acids of p62 but not on the tyrosine residue in this region.

Synonyms: A170 antibody|EBI 3 associated protein of 60 kDa antibody|EBI 3 associated protein p60 antibody|EBI3 associated protein of 60 kDa antibody|EBI3 associated protein p60 antibody| EBI3-associated protein of 60 kDa antibody|EBIAP antibody|MGC127197 antibody|ORCA antibody| OSF-6 antibody|Osi antibody|OSIL antibody|Oxidative stress induced like antibody|p60 antibody|p62 antibody|p62B antibody|Paget disease of bone 3 antibody|PDB 3 antibody|PDB3 antibody|Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa antibody| Phosphotyrosine independent ligand for the Lck SH2 domain p62 antibody|Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa antibody|PKC-zeta-interacting protein antibody|Protein kinase C-zeta-interacting protein antibody|Sequestosome 1 antibody| Sequestosome-1 antibody|SQSTM 1 antibody|SQSTM_HUMAN antibody|Sqstm1 antibody|STAP antibody|STONE14 antibody|Ubiquitin binding protein p62 antibody|Ubiquitin-binding protein p62 antibody|ZIP 3 antibody|ZIP antibody|ZIP3 antibody
Gene ID 8878
UniProt Q13501
Pathways NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy

Application Details

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Handling References for anti-SQSTM1 antibody (ABIN3043937) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for SQSTM1/p62 is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions For Research Use only

Handling

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details References for anti-SQSTM1 antibody (ABIN3043937) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-SQSTM1 antibody (ABIN3043937)

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Handling Images back to top
Product cited in:

Yu, Li, Wang, He, Ding, Zhang, Yu, Shi, Cui, Wang, Wang, Sun, Zhang, Du, Zhu: "Interferon regulatory factor-1 activates autophagy to aggravate hepatic ischemia-reperfusion injury via the P38/P62 pathway in mice." in: Scientific reports, Vol. 7, pp. 43684, 2018

Song, Hu, Li, Zheng, Wang, Guo, Shi, Liu: "Suppression of the toll-like receptor 7-dependent type I interferon production pathway by autophagy resulting from enterovirus 71 and coxsackievirus A16 infections facilitates their replication." in: Archives of virology, Vol. 163, Issue 1, pp. 135-144, 2018

Jeong, He, Nohara, Park, Shin, Kim, Shimomura, Koike, Yoo, Chen: "Dual attenuation of proteasomal and autophagic BMAL1 degradation in Clock Δ19/+ mice contributes to improved glucose homeostasis." in: Scientific reports, Vol. 5, pp. 12801, 2017

He, Wang, Han, Jin, Li, Wu, Ma, Cheng, Tang, Yang, Liu: "Nrf2 signalling and autophagy are involved in diabetes mellitus-induced defects in the development of mouse placenta." in: Open biology, Vol. 6, Issue 7, 2017

Li, Jen, Wu, Lee, Fang, Quigley, Lee, Wang, Zhou, Vergnes, Chen, Li, Reue, Ann, Hsiai: "Disturbed Flow Induces Autophagy, but Impairs Autophagic Flux to Perturb Mitochondrial Homeostasis." in: Antioxidants & redox signaling, Vol. 23, Issue 15, pp. 1207-19, 2016

Images

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Handling References for anti-SQSTM1 antibody (ABIN3043937) back to top
Supplier Images
Western Blotting (WB) image for anti-Sequestosome 1 (SQSTM1) (AA 69-96), (N-Term) antibody (ABIN3043937) anti-Sequestosome 1 (SQSTM1) (AA 69-96), (N-Term) antibody
Immunohistochemistry (IHC) image for anti-Sequestosome 1 (SQSTM1) (AA 69-96), (N-Term) antibody (ABIN3043937) Anti- SQSTM1 Picoband antibody,IHC(F) IHC(F): Rat Spleen Tissue
Immunohistochemistry (IHC) image for anti-Sequestosome 1 (SQSTM1) (AA 69-96), (N-Term) antibody (ABIN3043937) Anti- SQSTM1 Picoband antibody,IHC(F) IHC(F): Mouse Spleen Tissue
Immunohistochemistry (IHC) image for anti-Sequestosome 1 (SQSTM1) (AA 69-96), (N-Term) antibody (ABIN3043937) Anti- SQSTM1 Picoband antibody,IHC(P) IHC(P): Mouse Thymus Tissue
Immunohistochemistry (IHC) image for anti-Sequestosome 1 (SQSTM1) (AA 69-96), (N-Term) antibody (ABIN3043937) Anti- SQSTM1 Picoband antibody,IHC(P) IHC(P): Rat Spleen Tissue
Immunohistochemistry (IHC) image for anti-Sequestosome 1 (SQSTM1) (AA 69-96), (N-Term) antibody (ABIN3043937) Anti- SQSTM1 Picoband antibody,IHC(P) IHC(P): Human Lung Cancer Tissue