anti-Mouse (Murine) SQSTM1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended SQSTM1 Antibody (supplied by: Log in to see )

Antigen
Sequestosome 1 (SQSTM1) Antibodies
  • SQSTM1
  • sqstm1
  • MGC79491
  • A170
  • OSIL
  • PDB3
  • ZIP3
  • p60
  • p62
  • p62B
  • OSF-6
  • Osi
  • STAP
  • ZIP
  • sb:cb621
  • zgc:85784
  • sequestosome 1
  • sequestosome 1 L homeolog
  • SQSTM1
  • sqstm1
  • Sqstm1
  • sqstm1.L
Epitope
Internal Region
68
42
36
36
24
20
19
16
15
14
12
12
11
9
9
8
8
6
5
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Bee (Apiformes), Human, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)
372
190
135
8
5
5
5
4
4
4
2
2
2
2
1
1
1
1
Host
Rabbit
312
51
6
3
Clonality
Polyclonal
Conjugate
This SQSTM1 antibody is un-conjugated
19
17
16
12
12
12
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
309
120
102
96
71
69
38
35
28
22
17
13
4
3
2
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Catalog No. ABIN2854836
$ 466.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
23.5 ABIN3043937 IHC (fro) IHC (p) WB Rabbit IgG AA 69-96, N-Term Log in to see Polyclonal 5
20.5 ABIN6263985 ELISA ICC IF IHC (p) WB Rabbit IgG Log in to see Polyclonal 5
20.5 ABIN682153 IF (p) IHC (p) WB Rabbit IgG AA 80-130 Log in to see Polyclonal 4
11.116879 ABIN615711 EIA IHC (p) WB Rabbit C-Term Log in to see Polyclonal 0
7 ABIN768977 IHC IHC (p) WB Rabbit AA 323-372 Log in to see Polyclonal 0
7 ABIN604704 ELISA IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
4 ABIN1739629 IHC (p) ELISA WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN5553898 EIA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN334048 ICC IHC IHC (fro) IHC (p) WB Guinea Pig IgG AA 421-440 Log in to see Polyclonal 0
1 ABIN682162 IHC (p) WB HRP Rabbit IgG AA 80-130 Log in to see Polyclonal 0
1 ABIN682155 IHC (p) WB Biotin Rabbit IgG AA 80-130 Log in to see Polyclonal 0
1 ABIN1920949 IF IHC IHC (p) IP WB Rabbit IgG AA 375-425 Log in to see Polyclonal 0
1 ABIN1920950 IF IHC IHC (p) IP WB Rabbit IgG AA 425-440 Log in to see Polyclonal 0

General

Antigen Sequestosome 1 (SQSTM1) Antibodies
Epitope Internal Region
(68), (42), (36), (36), (24), (20), (19), (16), (15), (14), (12), (12), (11), (9), (9), (8), (8), (6), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Bee (Apiformes), Human, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)
(372), (190), (135), (8), (5), (5), (5), (4), (4), (4), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(312), (51), (6), (3)
Clonality
Polyclonal
Conjugate This SQSTM1 antibody is un-conjugated
(19), (17), (16), (12), (12), (12), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(309), (120), (102), (96), (71), (69), (38), (35), (28), (22), (17), (13), (4), (3), (2)
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Product Details anti-SQSTM1 Antibody

Target Details SQSTM1 Application Details Handling Images
Cross-Reactivity Rhesus Monkey
Cross-Reactivity (Details) Rhesus Monkey (93 %)
Characteristics Rabbit Polyclonal antibody to SQSTM1 (sequestosome 1)
SQSTM1 antibody [N3C1], Internal
Purification Purified by antigen-affinity chromatography.
Immunogen Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human SQSTM1. The exact sequence is proprietary.
Isotype IgG
Plasmids, Primers & others

Target Details SQSTM1

Product Details anti-SQSTM1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name SQSTM1 (SQSTM1 Antibody Abstract)
Background This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

Cellular Localization: Cytoplasm , Late endosome , Nucleus
Molecular Weight 48 kDa
Gene ID 8878
Pathways NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy

Application Details

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Handling Images back to top
Application Notes Suggested dilution Reference Flow cytometry/FACS 1:50-1:200* ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceFlow cytometry/FACS1:50-1:200* ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
Comment

Positive Control: A549 , NIH-3T3 , JC , BCL-1 , PC-12 , Rat2 , Huh7 (untreated) , Huh7 ( 3uM Thapsigargin treatment for 12hr) , *HeLa

Restrictions For Research Use only

Handling

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 1XPBS, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-SQSTM1 Antibody Target Details SQSTM1 Application Details Handling back to top
Supplier Images
Immunoprecipitation (IP) image for anti-Sequestosome 1 (SQSTM1) (Internal Region) antibody (ABIN2854836) IP Image Immunoprecipitation of SQSTM1 protein from HeLa whole cell extracts using 5 ...
Immunofluorescence (IF) image for anti-Sequestosome 1 (SQSTM1) (Internal Region) antibody (ABIN2854836) ICC/IF Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome...
Western Blotting (WB) image for anti-Sequestosome 1 (SQSTM1) (Internal Region) antibody (ABIN2854836) WB Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by western blot anal...
Immunohistochemistry (IHC) image for anti-Sequestosome 1 (SQSTM1) (Internal Region) antibody (ABIN2854836) IHC-P Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at cytoplasm on h...
Western Blotting (WB) image for anti-Sequestosome 1 (SQSTM1) (Internal Region) antibody (ABIN2854836) WB Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by Western blot anal...
Western Blotting (WB) image for anti-Sequestosome 1 (SQSTM1) (Internal Region) antibody (ABIN2854836) WB Image SQSTM1 antibody detects SQSTM1 protein by western blot analysis. Whole cell ...
Western Blotting (WB) image for anti-Sequestosome 1 (SQSTM1) (Internal Region) antibody (ABIN2854836) WB Image Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE antibody diluted at...
Immunofluorescence (IF) image for anti-Sequestosome 1 (SQSTM1) (Internal Region) antibody (ABIN2854836) ICC/IF Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome...
Flow Cytometry (FACS) image for anti-Sequestosome 1 (SQSTM1) (Internal Region) antibody (ABIN2854836) FACS Image SQSTM1 antibody [N3C1], Internal , detects SQSTM1 protein by flow cytometr...