anti-Dog (Canine) Tumor Necrosis Factor antibody for Immunoprecipitation

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • lipopolysaccharide induced TNF factor
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LITAF
  • LOC103694380
  • tnfa
Reactivity
Cat (Feline), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
1502
552
483
224
216
197
196
62
55
42
31
18
13
13
9
7
6
4
4
4
3
3
2
1
1
1
Host
Mouse
1150
573
178
48
11
10
8
7
5
2
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is conjugated to Alexa Fluor 488
192
103
83
63
61
39
32
31
28
26
26
26
26
25
25
25
24
20
13
11
11
11
11
11
11
11
11
11
11
11
11
11
10
10
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
912
892
793
489
397
314
219
175
132
121
93
67
48
42
26
26
24
17
17
12
10
9
9
7
6
6
6
5
5
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN4360976
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4360975 ELISA FACS ICC IF IHC (fro) IHC (p) IP Alexa Fluor 405 Mouse IgG1 kappa Log in to see SPM543 0
1 ABIN4360978 ELISA FACS ICC IF IHC (fro) IHC (p) IP Alexa Fluor 700 Mouse IgG1 kappa Log in to see SPM543 0
1 ABIN4360977 ELISA FACS ICC IF IHC (fro) IHC (p) IP Alexa Fluor 647 Mouse IgG1 kappa Log in to see SPM543 0
1 ABIN4360990 ELISA FACS ICC IF IHC (fro) IHC (p) IP DyLight 405 Mouse IgG1 kappa Log in to see SPM543 0
1 ABIN4360982 ELISA FACS ICC IF IHC (fro) IHC (p) IP DyLight 680 Mouse IgG1 kappa Log in to see SPM543 0
1 ABIN4360981 ELISA FACS ICC IF IHC (fro) IHC (p) IP DyLight 650 Mouse IgG1 kappa Log in to see SPM543 0
1 ABIN4360986 FACS ICC IF IHC (fro) IHC (p) IP PerCP Mouse IgG1 kappa Log in to see SPM543 0
1 ABIN4360989 ELISA FACS ICC IF IHC (fro) IHC (p) IP DyLight 350 Mouse IgG1 kappa Log in to see SPM543 0
1 ABIN4360983 ELISA FACS ICC IF IHC (fro) IHC (p) IP DyLight 488 Mouse IgG1 kappa Log in to see SPM543 0
1 ABIN4360988 ELISA FACS ICC IF IHC (fro) IHC (p) IP DyLight 550 Mouse IgG1 kappa Log in to see SPM543 0
1 ABIN4360985 ELISA FACS ICC IF IHC (fro) IHC (p) IP DyLight 755 Mouse IgG1 kappa Log in to see SPM543 0
1 ABIN4360972 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706 0
1 ABIN4360966 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa Log in to see P-T2 0
1 ABIN4360974 CyTOF FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM Log in to see TNF706-P-T2 0
1 ABIN4360962 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa Log in to see 4C6-H8 0
1 ABIN4360960 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP Mouse IgG1 kappa Log in to see SPM543 0
1 ABIN2904355 IF/ICC IHC IP WB Rabbit AA 63-233 Log in to see Polyclonal 0

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Cat (Feline), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
(1502), (552), (483), (224), (216), (197), (196), (62), (55), (42), (31), (18), (13), (13), (9), (7), (6), (4), (4), (4), (3), (3), (2), (1), (1), (1)
Host Mouse
(1150), (573), (178), (48), (11), (10), (8), (7), (5), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is conjugated to Alexa Fluor 488
(192), (103), (83), (63), (61), (39), (32), (31), (28), (26), (26), (26), (26), (25), (25), (25), (24), (20), (13), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (10), (10), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(912), (892), (793), (489), (397), (314), (219), (175), (132), (121), (93), (67), (48), (42), (26), (26), (24), (17), (17), (12), (10), (9), (9), (7), (6), (6), (6), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Specificity Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
Purification Protein A purified
Immunogen A hexadecapeptide corresponding to aa115-130 of human TNF-alpha, conjugated to thyroglobulin
Clone SPM543
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background Gene Symbol: TNF
Molecular Weight Theoretical MW: 17 kDa
Gene ID 7124
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-FrozenImmunohistochemistry (Staining of paraffin tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Format Liquid
Buffer 50 mM Sodium Borate
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C in the dark.