anti-Mouse (Murine) Tumor Necrosis Factor antibody for Functional Studies

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • lipopolysaccharide induced TNF factor
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LITAF
  • LOC103694380
  • tnfa
Reactivity
Mouse (Murine), Rat (Rattus)
1313
479
378
156
148
129
128
62
55
42
31
18
14
13
9
7
6
4
4
4
3
3
2
1
1
1
Host
Rat
976
558
178
48
11
10
8
7
5
3
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
181
103
73
52
50
33
31
28
28
26
26
26
26
25
25
25
24
13
10
10
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
891
881
640
366
336
242
238
197
136
129
94
66
48
29
28
26
25
18
14
11
9
9
7
6
6
6
5
5
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN119358
$ 555.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2476888 Func FACS ELISA WB Rat IgG1 Log in to see MP6-XT3 4
1 ABIN2476889 Func IHC (fro) FACS ELISA Rat IgG1 Log in to see MP6-XT22 4
1 ABIN1109383 Func EIA IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see 52B83 4
1 ABIN118027 EIA Func IHC (fro) IHC (p) IP RIA WB Rabbit Log in to see Polyclonal 2
1 ABIN598275 Func FACS Rat IgD Log in to see V1Q 0
1 ABIN1834916 Func FACS ELISA WB Rat IgG1 Log in to see MP6-XT3 0
1 ABIN2476875 Func ELISA WB Rabbit IgG Log in to see Polyclonal 4
1 ABIN602481 ELISA FACS Func IHC IHC (fro) Rat IgG1 Log in to see 0
1 ABIN1109409 Func Rat IgD Log in to see V1q 5
1 ABIN118025 EIA Func IHC (fro) IP RIA WB Rabbit Log in to see Polyclonal 2
1 ABIN1109389 Func Rat IgD Log in to see V1q 2
1 ABIN1109399 Func EIA IHC (fro) IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN115913 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal 0
1 ABIN115914 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal 0
1 ABIN2191760 Func FACS Rat IgD Log in to see V1q 4
1 ABIN641066 Func ELISA WB Rabbit IgG Log in to see 0
1 ABIN2192160 Func IA FACS IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
1 ABIN639415 Func IHC (fro) FACS ELISA Rat Log in to see 0
1 ABIN639313 Func FACS ELISA Rat Log in to see 0

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Mouse (Murine), Rat (Rattus)
(1313), (479), (378), (156), (148), (129), (128), (62), (55), (42), (31), (18), (14), (13), (9), (7), (6), (4), (4), (4), (3), (3), (2), (1), (1), (1)
Host Rat
(976), (558), (178), (48), (11), (10), (8), (7), (5), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(181), (103), (73), (52), (50), (33), (31), (28), (28), (26), (26), (26), (26), (25), (25), (25), (24), (13), (10), (10), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(891), (881), (640), (366), (336), (242), (238), (197), (136), (129), (94), (66), (48), (29), (28), (26), (25), (18), (14), (11), (9), (9), (7), (6), (6), (6), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN119358) Images
Purification Affinity Chromatography on Protein G
Endotoxin Level less than 0.01 EU/μg
Immunogen Recombinant Murine TNF alpha expressed in E. coli.
Clone MP6-XT22
Isotype IgG1

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN119358) Images back to top
Antigen
Alternative Name Tumor Necrosis Factor (TNF-Alpha) (TNF Antibody Abstract)
Background TNF alpha is a 17 KDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26KDa integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumour cell lines, and has an important immuno-regulatory role.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2
Gene ID 10090
UniProt P06804
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor antibody (ABIN119358) Images back to top
Application Notes Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, Membrane permeabilisation is required for this application. ELISA: 1 μg/mL - 10 μg/mL. Functional Assays. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor antibody (ABIN119358) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 without preservatives., None, None
Preservative Without preservative
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Store the antibody at 2 - 8 °C. DO NOT FREEZE!

References for anti-Tumor Necrosis Factor antibody (ABIN119358)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Background publications

Srinivas, Lovell, Skipper, Bennington, Kurban, Smith: "Ammonia absorption in calcium graphite intercalation compound: in situ neutron diffraction, Raman spectroscopy and magnetization." in: Physical chemistry chemical physics : PCCP, Vol. 12, Issue 23, pp. 6253-9, 2010

Ruan, Kang, Pei, Le: "Amyloid deposition and inflammation in APPswe/PS1dE9 mouse model of Alzheimer's disease." in: Current Alzheimer research, Vol. 6, Issue 6, pp. 531-40, 2009

Hostager, Bishop: "Role of TNF receptor-associated factor 2 in the activation of IgM secretion by CD40 and CD120b." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 7, pp. 3318-22, 2002

Scanga, Mohan, Joseph, Yu, Chan, Flynn: "Reactivation of latent tuberculosis: variations on the Cornell murine model." in: Infection and immunity, Vol. 67, Issue 9, pp. 4531-8, 1999

Ross, Williams, Mason, Mauri, Marinova-Mutafchieva, Malfait, Maini, Feldmann: "Suppression of TNF-alpha expression, inhibition of Th1 activity, and amelioration of collagen-induced arthritis by rolipram." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 12, pp. 6253-9, 1998

Yang, Kawamura, Zhu, Mitsuyama: "Involvement of natural killer cells in nitric oxide production by spleen cells after stimulation with Mycobacterium bovis BCG. Study of the mechanism of the different abilities of viable and killed BCG." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 12, pp. 5728-35, 1996

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992