anti-Mouse (Murine) Tumor Necrosis Factor antibody for Functional Studies

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LOC103694380
  • tnfa
Reactivity
Mouse (Murine)
1365
522
456
221
217
197
196
59
56
43
32
18
15
9
7
6
6
5
4
4
4
4
3
2
2
1
1
1
1
Host
Rat
1045
550
134
52
11
11
8
7
5
2
1
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
181
92
68
54
49
24
22
22
20
20
18
18
18
18
18
18
18
17
11
11
11
11
11
11
11
11
11
11
11
11
11
10
10
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS)
814
813
744
468
400
260
196
163
131
114
96
66
48
42
27
26
25
17
11
9
9
9
8
7
6
6
6
5
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Catalog No. ABIN2191760
$ 647.55
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
16.963114 ABIN2192160 Func IA FACS IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
8.5 ABIN2476888 Func FACS ELISA WB Rat IgG1 Log in to see MP6-XT3 4
5.5 ABIN2476889 Func IHC (fro) FACS ELISA Rat IgG1 Log in to see MP6-XT22 4
5.5 ABIN2476875 Func ELISA WB Rabbit IgG Log in to see Polyclonal 4
1 ABIN119358 EIA Func FACS IHC (fro) IHC (p) Rat IgG1 Log in to see MP6-XT22 7
1 ABIN1109383 Func EIA IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see 52B83 4
1 ABIN118027 EIA Func IHC (fro) IHC (p) IP RIA WB Rabbit Log in to see Polyclonal 2
1 ABIN1834916 Func FACS ELISA WB Rat IgG1 Log in to see MP6-XT3 0
1 ABIN598275 Func FACS Rat IgD Log in to see V1Q 0
1 ABIN602481 ELISA FACS Func IHC IHC (fro) Rat IgG1 Log in to see 0
1 ABIN1109409 Func Rat IgD Log in to see V1q 5
1 ABIN118025 EIA Func IHC (fro) IP RIA WB Rabbit Log in to see Polyclonal 2
1 ABIN641066 Func ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1109389 Func Rat IgD Log in to see V1q 2
1 ABIN1109399 Func EIA IHC (fro) IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN115914 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal 0
1 ABIN115913 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal 0
1 ABIN639415 Func IHC (fro) FACS ELISA Rat Log in to see 0
1 ABIN639313 Func FACS ELISA Rat Log in to see 0

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Mouse (Murine)
(1365), (522), (456), (221), (217), (197), (196), (59), (56), (43), (32), (18), (15), (9), (7), (6), (6), (5), (4), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1)
Host Rat
(1045), (550), (134), (52), (11), (11), (8), (7), (5), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(181), (92), (68), (54), (49), (24), (22), (22), (20), (20), (18), (18), (18), (18), (18), (18), (18), (17), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (10), (10), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS)
(814), (813), (744), (468), (400), (260), (196), (163), (131), (114), (96), (66), (48), (42), (27), (26), (25), (17), (11), (9), (9), (9), (8), (7), (6), (6), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN2191760) Images
Cross-Reactivity (Details) Cross reactivity: Chinese hamster TNF-α : Yes, Receptor-bound mouse TNF-α : No
Sterility 0.2 μm filtered
Clone V1q
Isotype IgD
Plasmids, Primers & others

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN2191760) Images back to top
Antigen
Alternative Name Tumor Necrosis Factor alpha (TNF Antibody Abstract)
Background The monoclonal antibody V1q recognizes mouse tumor necrosis factor alpha (TNF-α). TNF-α is the prototype cytokine of the family of TNF-related ligands, which are based on structural and functional homologies. TNF-α is synthesized as type II transmembrane protein. TNF-α can be recognized by two different membrane receptors, namely TNF-R1 and TNF-R2. TNF-α is present in a membrane-bound (tmTNF) as well as soluble form (sTNF). The membrane-bound form of TNF-α is recognized by both TNF receptors with high affinity, whereas the soluble form is recognized more superiorly by TNF-R1. TNF-α is produced by many different cell types including macrophages, T lymphocytes, NK cells, neutrophils and endothelial cells. Cells differ in the expression of the two TNF-receptors and sTNF versus tmTNF, respectively. TNF-α, a homotrimeric 17 kDa protein, is a potent mediator of inflammatory and metabolic functions. TNF-α was originally detected as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Furthermore, TNF-α has been implied as central mediator in shock induced by gram negative micro-organisms. TNF-α induces on its turn the production of many other cytokines. Furthermore, TNF-α has been found in inflammatory foci such as synovial effusions in rheumatoid arthritis, systemic circulation in septic shock, parasitemia and rejection of renal transplants. The monoclonal antibody V1q recognizes both natural and recombinant TNF-α and shows neutralizing activity. Aliases TNF, TNF-SF2, DIF, cachectin, TNF-α, tumor necrosis factor ligand superfamily member 2 Immunogen Cytotoxin purified from conA induced T cell clone 29
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor antibody (ABIN2191760) Images back to top
Application Notes For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Positive Monocytes/macrophages control
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor antibody (ABIN2191760) Images back to top
Buffer PBS, containing 0.1 % bovine serum albumin.
Storage 4 °C
Storage Comment Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

References for anti-Tumor Necrosis Factor antibody (ABIN2191760)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Rajashekhar, Grow, Willuweit, Patterson, Clauss: "Divergent and convergent effects on gene expression and function in acute versus chronic endothelial activation." in: Physiological genomics, Vol. 31, Issue 1, pp. 104-13, 2007

Demjen, Klussmann, Kleber, Zuliani, Stieltjes, Metzger, Hirt, Walczak, Falk, Essig, Edler, Krammer, Martin-Villalba: "Neutralization of CD95 ligand promotes regeneration and functional recovery after spinal cord injury." in: Nature medicine, Vol. 10, Issue 4, pp. 389-95, 2004

Gerspach, Götz, Zimmermann, Kolle, Böttinger, Grell: "Detection of membrane-bound tumor necrosis factor (TNF): an analysis of TNF-specific reagents." in: Microscopy research and technique, Vol. 50, Issue 3, pp. 243-50, 2000

Echtenacher, Falk, Männel, Krammer: "Requirement of endogenous tumor necrosis factor/cachectin for recovery from experimental peritonitis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 11, pp. 3762-6, 1991