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|Antigen||High Mobility Group AT-Hook 1 (HMGA1) Antibodies|
|Epitope||AA 9-21, Internal Region Alternatives|
|Reactivity||Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus) Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
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|Specificity||This antibody is expected to recognize reported isoforms apha (NP_665906.1, NP_665908.1, NP_665911.1) and beta (NP_002122.1, NP_665910.1, NP_665912.1). This antibody is not expected to cross-react with HMGA2.|
Species reactivity (expected):Mouse, Rat, Canine (Dog), Bovine, Pig.
Species reactivity (tested):Human.
|Purification||Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.|
|Immunogen||Peptide from the internal region (near N Terminus) of the protein sequence according to NP_665906.1, NP_002122.1. Genename: HMGA1|
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|Alternative Name||HMGA1 / HMGIY (HMGA1 Antibody Abstract)|
|Background||HMGA1 (HMGI/Y) is a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. There are two splice isoforms of HMGA1 - the full length HMGA1a/HMGI and the shortened form HMGA1b/HMGY. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. HMGA1 is frequently acetylated and is found in the nucleus. Interacts with HIV-1 pre-integration complex.Synonyms: High mobility group AT-hook protein 1, High mobility group protein A1, High mobility group protein HMG-I/HMG-Y, High mobility group protein R|
|Pathways||Nuclear Hormone Receptor Binding, Autophagy|
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Peptide ELISA: Detection Limit: 1/16000. Western blot: 0.5-1 μg/mL. Approx 24 kDa band observed in Human Pancreas (calculatedMW of 11.4 kDa/10.4 kDa according to NP_665906.1, NP_002122.1). The observed molecularweight corresponds to earlier findings with different antibodies from other commercialsources. This band was successfully blocked by incubation with the immunizing peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
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|Buffer||Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|