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|Antigen||Ataxia Telangiectasia and Rad3 Related (ATR) Antibodies|
|Epitope||Internal Region Alternatives|
|Reactivity||Dog (Canine), Fish, Human, Monkey, Mouse (Murine), Rat (Rattus), Xenopus laevis Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
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Product Details anti-ATR AntibodyTarget Details ATR Application Details Handling Images
|Specificity||This antibody is specific for ATR protein.|
Species reactivity (expected):Mouse, Rat, Monkey, Canine, Fish, Xenopus.
Species reactivity (tested):Human.
|Characteristics||Concentration: 1.28 mg/mL (by UV absorbance at 280 nm)|
|Immunogen||Synthetic peptide corresponding to an internal region of human ATR protein|
Target Details ATRProduct Details anti-ATR Antibody Application Details Handling Images back to top
|Alternative Name||ATR / FRP1 (ATR Antibody Abstract)|
|Background||Ataxia Telangiectasia Mutated (ATM) and Rad 3-related protein (ATR) is a phosphatidylinositol kinase (PK)-related kinase which functions in response to DNA damage and repair as well as at DNA replication checkpoints during the cell cycle. ATR activates checkpoint signaling upon genotoxic stresses, such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. ATR is a member of the DNA-PK kinase family and is closely related to ATM and DNAPK for which DNA stimulates the observed kinase activity. Chromosomal remodeling proteins have also been reported to associate with ATR complexes, including histone deacetylases (HDAC1, HDAC2 and CHD4). ATR is known to phosphorylate BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and TP53/p53 which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. ATR is a nuclear protein, but can also be found in PML nuclear bodies in certain cell types. ATR is recruited to chromatin during Sphase and redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.Synonyms: Ataxia telangiectasia and Rad3-related protein, FRAP-related protein 1, Serine/threonine-protein kinase ATR|
|Pathways||p53 Signaling, Apoptosis, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus|
Application DetailsProduct Details anti-ATR Antibody Target Details ATR Handling Images back to top
ELISA: 1/15,000 - 1/70,000. Western Blot: 1/1,000 - 1/5,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-ATR Antibody Target Details ATR Application Details Images back to top
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|