anti-Human BAX antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended BAX Antibody (supplied by: Log in to see )

Antigen
BCL2-Associated X Protein (BAX) Antibodies
  • BAX-ALPHA
  • bax-A
  • xBax
  • xlbax
  • BAX
  • bax
  • fj16e01
  • wu:fc50b10
  • wu:fj16e01
  • BCL2L4
  • BCL2 associated X, apoptosis regulator
  • BCL2-associated X protein L homeolog
  • BCL2-associated X protein
  • bcl2-associated X protein, a
  • BAX
  • bax.L
  • bax
  • baxa
  • Bax
Epitope
AA 3-16
89
76
31
22
21
19
15
13
12
12
11
10
9
9
9
7
7
5
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Monkey
556
233
214
127
44
31
28
23
21
11
7
7
6
5
3
3
3
2
2
2
1
1
1
1
1
Host
Mouse
348
242
4
2
Clonality
Monoclonal
Conjugate
This BAX antibody is conjugated to CF®770
19
15
14
12
11
9
8
6
5
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
454
230
194
167
142
140
113
47
41
40
32
26
13
5
5
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN6175302
$ 209.23
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN6175304 FACS IF IHC (f) WB CF®680 Mouse IgG1 AA 3-16 Log in to see 2D2 0
1 ABIN6175306 FACS IF IHC (f) WB CF®680R Mouse IgG1 AA 3-16 Log in to see 2D2 0
1 ABIN6175286 FACS IF IHC (f) WB CF®405S Mouse IgG1 AA 3-16 Log in to see 2D2 0
1 ABIN6175290 FACS IF IHC (f) WB CF®640R Mouse IgG1 AA 3-16 Log in to see 2D2 0
1 ABIN6175294 FACS IF IHC (f) WB CF®647 Mouse IgG1 AA 3-16 Log in to see 2D2 0
1 ABIN6175292 FACS IF IHC (f) WB CF®543 Mouse IgG1 AA 3-16 Log in to see 2D2 0
1 ABIN6175298 FACS IF IHC (f) WB CF®660R Mouse IgG1 AA 3-16 Log in to see 2D2 0
1 ABIN6175310 FACS IF IHC (f) WB CF®594 Mouse IgG1 AA 3-16 Log in to see 2D2 0
1 ABIN6175288 FACS IF IHC (f) WB CF405M Mouse IgG1 AA 3-16 Log in to see 2D2 0
1 ABIN6175296 FACS IF IHC (f) WB CF®555 Mouse IgG1 AA 3-16 Log in to see 2D2 0
1 ABIN6175300 FACS IF IHC (f) WB CF®568 Mouse IgG1 AA 3-16 Log in to see 2D2 0
1 ABIN6175308 FACS IF IHC (f) WB CF®488A Mouse IgG1 AA 3-16 Log in to see 2D2 0
1 ABIN6175321 FACS IF IHC (f) WB Mouse IgG1 AA 3-16 Log in to see 2D2 0
1 ABIN6175313 FACS IF IHC (f) WB Alkaline Phosphatase (AP) Mouse IgG1 AA 3-16 Log in to see 2D2 0
1 ABIN6175315 FACS IF IHC (f) WB Biotin Mouse IgG1 AA 3-16 Log in to see 2D2 0
1 ABIN6175312 FACS IF IHC (f) WB APC Mouse IgG1 AA 3-16 Log in to see 2D2 0
1 ABIN6175319 FACS IF IHC (f) WB PerCP Mouse IgG1 AA 3-16 Log in to see 2D2 0
1 ABIN6175320 FACS IF IHC (f) WB PE Mouse IgG1 AA 3-16 Log in to see 2D2 0
1 ABIN6175323 FACS IF IHC (f) WB Mouse IgG1 AA 3-16 Log in to see 2D2 0
1 ABIN6175326 FACS IF IHC (f) WB CF405M Mouse IgG1 Log in to see BAX-962 0

General

Antigen BCL2-Associated X Protein (BAX) Antibodies
Epitope AA 3-16
(89), (76), (31), (22), (21), (19), (15), (13), (12), (12), (11), (10), (9), (9), (9), (7), (7), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Monkey
(556), (233), (214), (127), (44), (31), (28), (23), (21), (11), (7), (7), (6), (5), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(348), (242), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BAX antibody is conjugated to CF®770
(19), (15), (14), (12), (11), (9), (8), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
(454), (230), (194), (167), (142), (140), (113), (47), (41), (40), (32), (26), (13), (5), (5), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-BAX Antibody

Target Details BAX Application Details Handling Images
Purpose Mouse Monoclonal anti-Bax (2D2), CF770 Conjugate
Characteristics Recognizes a protein of 21 kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Immunogen A synthetic peptide, aa 3-16 (Cys-GSGEQPRGGGPTSS) of human bax protein.
Clone 2D2
Isotype IgG1
Plasmids, Primers & others

Target Details BAX

Product Details anti-BAX Antibody Application Details Handling Images back to top
Antigen
Alternative Name Bax (BAX Antibody Abstract)
Molecular Weight 21 kDa
Gene ID 581, 624291
UniProt Q07812
Pathways p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded protein response

Application Details

Product Details anti-BAX Antibody Target Details BAX Handling Images back to top
Application Notes Does not react with mouse or rat, others not known
  • Immunohistology formalin-fixed 0.5-1 μg/mL
  • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
  • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
  • Immunofluorescence 1-2 μg/mL
  • Western blotting 0.5-1 μg/mL
  • Optimal dilution for a specific application should be determined by user
Comment

Jurkat, K562, HL-60, or HeLa Cells. Reed-Sternberg cells in Hodgkin s lymphoma.

Restrictions For Research Use only

Handling

Product Details anti-BAX Antibody Target Details BAX Application Details Images back to top
Format Liquid
Concentration 100 μg/mL
Buffer PBS/0.1 % BSA/0.05 % azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light