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|Antigen||Fas (TNF Receptor Superfamily, Member 6) (FAS) Antibodies|
|Conjugate||This FAS antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|6 references available|
|Supplier||Log in to see|
Product Details anti-FAS AntibodyTarget Details FAS Application Details Handling References for anti-FAS antibody (ABIN238438) Images
|Specificity||The antibody LT95 reacts with CD95 (Fas/APO-1), a 46 kDa single chain type I glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells. It seems that the antibody LT95 does not induce Fas mediated apoptosis, although it cross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human.|
|Characteristics||Synonyms: FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6, Tumor necrosis factor receptorsuperfamily member 6|
|Purification||Precipitation methods and Ion exchange chromatography.|
|Immunogen||HUT-78 human T cell lymphoma cell line|
Target Details FASProduct Details anti-FAS Antibody Application Details Handling References for anti-FAS antibody (ABIN238438) Images back to top
|Alternative Name||CD95 / FAS (FAS Antibody Abstract)|
|Background||CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.Synonyms: APT1, Apo-1 antigen, FAS1, FASLG receptor, TNFRSF6, Tumor necrosis factor receptor superfamily member 6|
|Pathways||p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity|
Application DetailsProduct Details anti-FAS Antibody Target Details FAS Handling References for anti-FAS antibody (ABIN238438) Images back to top
Flow Cytometry. Immunohistochemistry (paraffin sections). Positive tissue: tonsil
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-FAS Antibody Target Details FAS Application Details References for anti-FAS antibody (ABIN238438) Images back to top
|Buffer||Tris buffered saline (TBS), pH 8.0, 15 mM Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-FAS antibody (ABIN238438)Product Details anti-FAS Antibody Target Details FAS Application Details Handling Images back to top
Brumatti, Yon, Castro, Bueno-da-Silva, Jacysyn, Brunner, Amarante-Mendes: "Conversion of CD95 (Fas) Type II into Type I signaling by sub-lethal doses of cycloheximide." in: Experimental cell research, Vol. 314, Issue 3, pp. 554-63, 2008
Guo, Zhang, Tang, Cao: "Fas signal links innate and adaptive immunity by promoting dendritic-cell secretion of CC and CXC chemokines." in: Blood, Vol. 106, Issue 6, pp. 2033-41, 2005
Drosopoulos, Roberts, Cermak, Sasazuki, Shirasawa, Andera, Pintzas: "Transformation by oncogenic RAS sensitizes human colon cells to TRAIL-induced apoptosis by up-regulating death receptor 4 and death receptor 5 through a MEK-dependent pathway." in: The Journal of biological chemistry, Vol. 280, Issue 24, pp. 22856-67, 2005
Nakamura, Kakinuma, Amada, Miyata, Taniguchi, Koda, Sato: "Pyrazole derivatives as new potent and selective 20-hydroxy-5,8,11,14-eicosatetraenoic acid synthase inhibitors." in: Bioorganic & medicinal chemistry, Vol. 12, Issue 23, pp. 6209-19, 2004
Park, Thomsen, Frevert, Pham, Skerrett, Kiener, Liles: "Fas (CD95) induces proinflammatory cytokine responses by human monocytes and monocyte-derived macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 6209-16, 2003
Scaffidi, Fulda, Srinivasan, Friesen, Li, Tomaselli, Debatin, Krammer, Peter: "Two CD95 (APO-1/Fas) signaling pathways." in: The EMBO journal, Vol. 17, Issue 6, pp. 1675-87, 1998
ImagesProduct Details anti-FAS Antibody Target Details FAS Application Details Handling References for anti-FAS antibody (ABIN238438) back to top