anti-Human PAG1 antibody for Cytometry by Time of Flight

Recommended PAG1 Antibody (supplied by: Log in to see )

Antigen
phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) Antibodies
  • Cbp
  • F730007C19Rik
  • Pag
  • CBP
  • PAG
  • MGC80192
  • phosphoprotein associated with glycosphingolipid microdomains 1
  • phosphoprotein membrane anchor with glycosphingolipid microdomains 1
  • phosphoprotein membrane anchor with glycosphingolipid microdomains 1 L homeolog
  • Pag1
  • PAG1
  • pag1.L
  • pag1
Epitope
AA 235-280
37
26
16
4
3
2
2
2
1
1
Reactivity
Human
105
67
51
34
2
2
1
1
1
Host
Mouse
59
54
Clonality
Monoclonal
Conjugate
This PAG1 antibody is un-conjugated
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
74
59
47
32
21
13
11
6
3
1
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Catalog No. ABIN268975
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General

Antigen phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) Antibodies
Epitope AA 235-280
(37), (26), (16), (4), (3), (2), (2), (2), (1), (1)
Reactivity Human
(105), (67), (51), (34), (2), (2), (1), (1), (1)
Host Mouse
(59), (54)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PAG1 antibody is un-conjugated
(4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(74), (59), (47), (32), (21), (13), (11), (6), (3), (1)
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Product Details anti-PAG1 Antibody

Target Details PAG1 Application Details Handling Images
Specificity The antibody MEM-255 recognizes an epitope (aa 235-280) of Csk-binding protein (Cbp) located in the cytoplasmic domain, also known as protein associated with glycosphingolipid-enriched microdomains (PAG).
Purification Protein A purified
Immunogen Recombinant intracellular fragment, corresponding to amino acids 97-432 of Human Cbp/PAG (NM_018440.3).
Clone MEM-255
Isotype IgG2a
Plasmids, Primers & others

Target Details PAG1

Product Details anti-PAG1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PAG1 (PAG1 Antibody Abstract)
Background Gene Symbol: PAG1
Gene ID 55824
UniProt Q9NWQ8
Pathways p53 Signaling, TCR Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events

Application Details

Product Details anti-PAG1 Antibody Target Details PAG1 Handling Images back to top
Application Notes Western Blot 1:1000, Flow Cytometry 2 μg/mL, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:200, CyTOF-readyPurity is > 95 % (by SDS-PAGE). Flow Cytometry - intracellular staining, permeabilization required Western Blot - Csk binding protein is an ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane microdomains (rafts), which however migrates on SDS-PAGE gels anomalously as an 80 kDa molecule.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-PAG1 Antibody Target Details PAG1 Application Details Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS ( pH 7.4)
Buffer contains: 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

Images

Product Details anti-PAG1 Antibody Target Details PAG1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (AA 235-280) antibody (ABIN268975) Immunohistochemistry: PAG Antibody (MEM-255) [ABIN268975] - Cbp/PAG is expressed in g...