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|Antigen||serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1 (SERPINE1) Antibodies|
|Conjugate||This SERPINE1 antibody is un-conjugated Alternatives|
ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut), Western Blotting (WB)
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Product Details anti-SERPINE1 AntibodyTarget Details SERPINE1 Application Details Handling Images
E.coli derived recombinant Human PAI-1.
Type of Immunogen: Cell extract
|Plasmids, Primers & others|
Target Details SERPINE1Product Details anti-SERPINE1 Antibody Application Details Handling Images back to top
|Alternative Name||SERPINE1 / PAI-1 (SERPINE1 Antibody Abstract)|
Name/Gene ID: SERPINE1
Family: Serine Protease Inhibitor
Synonyms: SERPINE1, PAI1, PLANH1, PAI-1, Serpin E1
|Pathways||p53 Signaling, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration|
Application DetailsProduct Details anti-SERPINE1 Antibody Target Details SERPINE1 Handling Images back to top
Approved: ELISA, IHC, IHC-P (5 μg/mL), Neut (15 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: LS-B7483 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hPAI-1 (4.5 μg/mL), a concentration of at least 15 μg/mL of this antibody is required. ELISA: To detect hPAI-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human PAI-1 (LS-C104564) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPAI-1. Western Blot: To detect hPAI-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPAI-1 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Target Species of Antibody: Human
|Restrictions||For Research Use only|
HandlingProduct Details anti-SERPINE1 Antibody Target Details SERPINE1 Application Details Images back to top
|Buffer||Lyophilized from PBS, pH 7.2|
|Handling Advice||Avoid freeze/thaw cycles.|
|Storage||4 °C,-20 °C|
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
ImagesProduct Details anti-SERPINE1 Antibody Target Details SERPINE1 Application Details Handling back to top