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|Antigen||Cyclin-Dependent Kinase 2 (CDK2) Antibodies|
|Epitope||AA 81-298 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CDK2 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
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Product Details anti-CDK2 AntibodyTarget Details CDK2 Application Details Handling References for anti-CDK2 antibody (ABIN3043472) Images
|Purpose||Rabbit IgG polyclonal antibody for Cyclin-dependent kinase 2(CDK2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Cyclin-dependent kinase 2(CDK2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: cyclin-dependent kinase 2
Protein Name: Cyclin-dependent kinase 2
|Purification||Immunogen affinity purified.|
|Immunogen||E.coli-derived human Cdk2 recombinant protein (Position: E81-L298). Human Cdk2 shares 98.6% amino acid (aa) sequence identity with rat Cdk2.|
Target Details CDK2Product Details anti-CDK2 Antibody Application Details Handling References for anti-CDK2 antibody (ABIN3043472) Images back to top
|Alternative Name||CDK2 (CDK2 Antibody Abstract)|
CDK2, Cyclin-Dependent Kinase2, is also known as P33. The CDK2 protein was highly homologous to p34(CDC2) kinase and more significantly homologous to Xenopus Eg1 kinase, suggesting that CDK2 is the human homolog of Eg1. The CDK2 gene is mapped to 12q13, the same region to which the CDK4 gene maps. Human cyclin A binds independently to 2 kinases, p34(cdc2) or p33. In adenovirus-transformed cells, the viral E1A oncoprotein seems to associate with p33/cyclin A but not with p34(cdc2)/cyclin A. The gene for p33 shares 65 % sequence identity with p34(cdc2). P33(cdk2) plays a unique role in cell cycle regulation of vertebrate cells.
Synonyms: Cdc2 related protein kinase antibody|cdc2-related protein kinase antibody|Cdk 2 antibody| CDK2 antibody|CDK2_HUMAN antibody|Cell devision kinase 2 antibody|Cell division kinase 2 antibody|Cell division protein kinase 2 antibody|Cyclin dependent kinase 2 antibody|cyclin dependent kinase 2-alpha antibody|Cyclin-dependent kinase 2 antibody|p33 protein kinase antibody|p33(CDK2) antibody
|Pathways||PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA|
Application DetailsProduct Details anti-CDK2 Antibody Target Details CDK2 Handling References for anti-CDK2 antibody (ABIN3043472) Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-CDK2 Antibody Target Details CDK2 Application Details References for anti-CDK2 antibody (ABIN3043472) Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
References for anti-CDK2 antibody (ABIN3043472)Product Details anti-CDK2 Antibody Target Details CDK2 Application Details Handling Images back to top
|Product cited in:||
Ren, Huang, Xu, Yang, Yang, Hu: "Isoflavone lupiwighteone induces cytotoxic, apoptotic, and antiangiogenic activities in DU-145 prostate cancer cells." in: Anti-cancer drugs, Vol. 26, Issue 6, pp. 599-611, 2015
Zhou, Lu, Liu, Guo, Liu, Zhou, Yang, Mi, Xu: "Platycodin D induces tumor growth arrest by activating FOXO3a expression in prostate cancer in vitro and in vivo." in: Current cancer drug targets, Vol. 14, Issue 9, pp. 860-71, 2015
Zhao, Li, Gao, Wang, Yan, Zhan, Liu, Mao, Chen, Wang et al.: "Design, synthesis and biological evaluation of N-alkyl or aryl substituted isoindigo derivatives as potential dual cyclin-dependent kinase 2 (CDK2)/glycogen synthase kinase 3? (GSK-3?) ..." in: European journal of medicinal chemistry, Vol. 86, pp. 165-74, 2014
Cheng, Chen, Wang, Ding, Shi: "The effects of urokinase-type plasminogen activator (uPA) on cell proliferation and phenotypic transformation of rat mesangial cells induced by high glucose." in: Diabetes research and clinical practice, Vol. 103, Issue 3, pp. 489-95, 2014
Ye, Chen, Chen, Xie, Yang, Lou, Yu: "XJW20, a novel oxoindole derivative, induces G2/M arrest and apoptosis selectively in K562 leukemia cell line." in: Chemico-biological interactions, Vol. 183, Issue 1, pp. 133-41, 2009
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