anti-Human CHUK antibody for Cytometry by Time of Flight

Recommended CHUK Antibody (supplied by: Log in to see )

Antigen
conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
  • ikk
  • ikk1
  • ikka
  • ikkalpha
  • CHUK
  • IKBKA
  • IKK-alpha
  • IKK1
  • IKKA
  • NFKBIKA
  • TCF16
  • AI256658
  • Chuk1
  • Fbx24
  • Fbxo24
  • Ikka
  • zgc:56539
  • conserved helix-loop-helix ubiquitous kinase S homeolog
  • conserved helix-loop-helix ubiquitous kinase
  • chuk.S
  • CHUK
  • Chuk
  • chuk
Reactivity
Human, Mouse (Murine), Primate
356
166
116
19
12
11
10
7
6
5
5
4
2
1
Host
Mouse
282
75
14
1
Clonality
Monoclonal
Conjugate
This CHUK antibody is un-conjugated
15
11
11
9
9
8
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
329
163
120
76
61
45
38
28
26
18
9
5
3
2
2
2
1
1
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Catalog No. ABIN4324239
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General

Antigen conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
Reactivity Human, Mouse (Murine), Primate
(356), (166), (116), (19), (12), (11), (10), (7), (6), (5), (5), (4), (2), (1)
Host Mouse
(282), (75), (14), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CHUK antibody is un-conjugated
(15), (11), (11), (9), (9), (8), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
(329), (163), (120), (76), (61), (45), (38), (28), (26), (18), (9), (5), (3), (2), (2), (2), (1), (1)
Pubmed 43 references available
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Product Details anti-CHUK Antibody

Target Details CHUK Application Details Handling References for anti-CHUK antibody (ABIN4324239) Images
Specificity An 85 kDa band should be observed.
Purification Protein G purified
Sterility Sterile
Immunogen This antibody was raised against a His-tagged full-length human IKK alpha protein.
Clone 14A231
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details CHUK

Product Details anti-CHUK Antibody Application Details Handling References for anti-CHUK antibody (ABIN4324239) Images back to top
Antigen
Alternative Name IKK alpha (CHUK Antibody Abstract)
Background Gene Symbol: CHUK
Gene ID 1147
Pathways PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades

Application Details

Product Details anti-CHUK Antibody Target Details CHUK Handling References for anti-CHUK antibody (ABIN4324239) Images back to top
Application Notes Western Blot 1 μg/mL, Simple Western, Flow Cytometry 1 μL/1 million cells, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1-2 μg/mL, Immunohistochemistry-Paraffin 5 μg/mL, Immunohistochemistry-Frozen, Flow (Intracellular), CyTOF-readyUse in Flow Intracellular reported in scientific literature (PMID 24804954) Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 25133425)In Simple Western only 10-15 μL of the recommended dilution is used per data point.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CHUK Antibody Target Details CHUK Application Details References for anti-CHUK antibody (ABIN4324239) Images back to top
Format Liquid
Buffer 0.2 mL sterile PBS
Buffer contains: No Preservative
Preservative Without preservative
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-CHUK antibody (ABIN4324239)

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling Images back to top
Product cited in:

Fang, Jeong, Long, Park, Wang, Shuai, Wei, Li, Li, Zhang, Duran, Lo, Tsao, Glaser, Luo, Feng, Tian, Luo: "IKKα-mediated biogenesis of miR-196a through interaction with Drosha regulates the sensitivity of cancer cells to radiotherapy." in: Cell death and differentiation, Vol. 23, Issue 9, pp. 1471-82, 2016

Delhase, Kim, Lee, Naiki-Ito, Chen, Ahn, Murata, Kim, Lautsch, Kobayashi, Shirai, Karin, Nakanishi: "TANK-binding kinase 1 (TBK1) controls cell survival through PAI-2/serpinB2 and transglutaminase 2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 4, pp. E177-86, 2012

Bakkar, Ladner, Canan, Liyanarachchi, Bal, Pant, Periasamy, Li, Janssen, Guttridge: "IKK? and alternative NF-?B regulate PGC-1? to promote oxidative muscle metabolism." in: The Journal of cell biology, Vol. 196, Issue 4, pp. 497-511, 2012

Cui, Li, Zhu, Liu, Songyang, Wang, Wang: "NLRP4 negatively regulates type I interferon signaling by targeting the kinase TBK1 for degradation via the ubiquitin ligase DTX4." in: Nature immunology, Vol. 13, Issue 4, pp. 387-95, 2012

Phromnoi, Reuter, Sung, Prasad, Kannappan, Yadav, Chanmahasathien, Limtrakul, Aggarwal et al.: "A novel pentamethoxyflavone down-regulates tumor cell survival and proliferative and angiogenic gene products through inhibition of IκB kinase activation and sensitizes tumor cells to apoptosis by ..." in: Molecular pharmacology, Vol. 79, Issue 2, pp. 279-89, 2011

Harikumar, Sung, Pandey, Guha, Krishnan, Aggarwal: "Escin, a pentacyclic triterpene, chemosensitizes human tumor cells through inhibition of nuclear factor-kappaB signaling pathway." in: Molecular pharmacology, Vol. 77, Issue 5, pp. 818-27, 2010

Huang, Ren, Guan, Jiang, Cheng: "HTLV-1 Tax is a critical lipid raft modulator that hijacks IkappaB kinases to the microdomains for persistent activation of NF-kappaB." in: The Journal of biological chemistry, Vol. 284, Issue 10, pp. 6208-17, 2009

Kasinski, Du, Thomas, Zhao, Sun, Khuri, Wang, Shoji, Sun, Snyder, Liotta, Fu: "Inhibition of IkappaB kinase-nuclear factor-kappaB signaling pathway by 3,5-bis(2-flurobenzylidene)piperidin-4-one (EF24), a novel monoketone analog of curcumin." in: Molecular pharmacology, Vol. 74, Issue 3, pp. 654-61, 2008

Pandey, Sung, Kunnumakkara, Sethi, Chaturvedi, Aggarwal: "Berberine modifies cysteine 179 of IkappaBalpha kinase, suppresses nuclear factor-kappaB-regulated antiapoptotic gene products, and potentiates apoptosis." in: Cancer research, Vol. 68, Issue 13, pp. 5370-9, 2008

Otipoby, Sasaki, Schmidt-Supprian, Patke, Gareus, Pasparakis, Tarakhovsky, Rajewsky: "BAFF activates Akt and Erk through BAFF-R in an IKK1-dependent manner in primary mouse B cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 34, pp. 12435-8, 2008 (Sample species: Mouse (Murine)). Further details: Western Blotting

Eckmann, Nebelsiek, Fingerle, Dann, Mages, Lang, Robine, Kagnoff, Schmid, Karin, Arkan, Greten: "Opposing functions of IKKbeta during acute and chronic intestinal inflammation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 39, pp. 15058-63, 2008 (Sample species: Mouse (Murine)). Further details: Western Blotting

Kunnumakkara, Ichikawa, Anand, Mohankumar, Hema, Nair, Aggarwal et al.: "Coronarin D, a labdane diterpene, inhibits both constitutive and inducible nuclear factor-kappa B pathway activation, leading to potentiation of apoptosis, inhibition of invasion, and suppression of ..." in: Molecular cancer therapeutics, Vol. 7, Issue 10, pp. 3306-17, 2008 (Sample species: Human). Further details: Western Blotting

Jackson-Bernitsas, Ichikawa, Takada, Myers, Lin, Darnay, Chaturvedi, Aggarwal: "Evidence that TNF-TNFR1-TRADD-TRAF2-RIP-TAK1-IKK pathway mediates constitutive NF-kappaB activation and proliferation in human head and neck squamous cell carcinoma." in: Oncogene, Vol. 26, Issue 10, pp. 1385-97, 2007

Beraza, Lüdde, Assmus, Roskams, Vander Borght, Trautwein: "Hepatocyte-specific IKK gamma/NEMO expression determines the degree of liver injury." in: Gastroenterology, Vol. 132, Issue 7, pp. 2504-17, 2007 (Sample species: Mouse (Murine)). Further details: Western Blotting

Vinolo, Sebban, Chaffotte, Israël, Courtois, Véron, Agou et al.: "A point mutation in NEMO associated with anhidrotic ectodermal dysplasia with immunodeficiency pathology results in destabilization of the oligomer and reduces lipopolysaccharide- and tumor necrosis ..." in: The Journal of biological chemistry, Vol. 281, Issue 10, pp. 6334-48, 2006

Ichikawa, Aggarwal: "Guggulsterone inhibits osteoclastogenesis induced by receptor activator of nuclear factor-kappaB ligand and by tumor cells by suppressing nuclear factor-kappaB activation." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 12, Issue 2, pp. 662-8, 2006

Sethi, Ahn, Sandur, Lin, Chaturvedi, Aggarwal: "Indirubin enhances tumor necrosis factor-induced apoptosis through modulation of nuclear factor-kappa B signaling pathway." in: The Journal of biological chemistry, Vol. 281, Issue 33, pp. 23425-35, 2006

Panopoulos, Harraz, Engelhardt, Zandi et al.: "Iron-mediated H2O2 production as a mechanism for cell type-specific inhibition of tumor necrosis factor alpha-induced but not interleukin-1beta-induced IkappaB kinase complex/nuclear factor-kappaB ..." in: The Journal of biological chemistry, Vol. 280, Issue 4, pp. 2912-23, 2005

Nonaka, Horie, Itoh, Watanabe, Yamamoto, Yamaoka: "Aberrant NF-kappaB2/p52 expression in Hodgkin/Reed-Sternberg cells and CD30-transformed rat fibroblasts." in: Oncogene, Vol. 24, Issue 24, pp. 3976-86, 2005

Ruocco, Maeda, Park, Lawrence, Hsu, Cao, Schett, Wagner, Karin: "I{kappa}B kinase (IKK){beta}, but not IKK{alpha}, is a critical mediator of osteoclast survival and is required for inflammation-induced bone loss." in: The Journal of experimental medicine, Vol. 201, Issue 10, pp. 1677-87, 2005

Images

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling References for anti-CHUK antibody (ABIN4324239) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibody (ABIN4324239) Flow Cytometry: IKK alpha Antibody (14A231) - Azide Free [NBP2-27409] - Intracellular...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibody (ABIN4324239) Immunohistochemistry-Paraffin: IKK alpha Antibody (14A231) - Azide Free [NBP2-27409] ...
Western Blotting (WB) image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibody (ABIN4324239) Western Blot: IKK alpha Antibody (14A231) - Azide Free [NBP2-27409] - analysis of IKK...
Simple Western (SimWes) image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibody (ABIN4324239) Simple Western: IKK alpha Antibody (14A231) - Azide Free [NBP2-27409] - Analysis usin...
Immunofluorescence (IF) image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibody (ABIN4324239) Immunocytochemistry/Immunofluorescence: IKK alpha Antibody (14A231) - Azide Free [NBP...