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|Antigen||conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies|
|Conjugate||This CHUK antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
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Product Details anti-CHUK AntibodyTarget Details CHUK Application Details Handling Images
|Specificity||IKK alpha antibody was raised against a peptide corresponding to amino acids 716 to 734 of human IKK alpha, which differs from corresponding murine sequence by four amino acids. This antibody has no cross response to IKKb or IKKg.|
|Purification||Affinity chromatography purified via peptide column|
|Immunogen||Human IKK alpha (C-Terminus 1) Peptide|
Target Details CHUKProduct Details anti-CHUK Antibody Application Details Handling Images back to top
|Alternative Name||IKKA (CHUK Antibody Abstract)|
|Background||Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa, and bacteria product LPS. NF-kappaB is associated with IkappaB proteins in the cell cytoplasm, which inhibit NF-kappaB activity. The long-sought IkappaB kinase (IKK), which phosphorylates IkappaB, and mediates IΚB degradation and NF-kappaB activation, was recently identified by several laboratories (1-5). IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKalpha and IKKbeta). IKKa and IKKbeta interact with each other and both are essential for the NF-kappaB activation. IKKalpha specifically phosphorylates IkB-alpha. IKKalpha is expressed in variety of human tissues.Synonyms: CHUK, Conserved helix-loop-helix ubiquitous kinase, I kappa-B kinase alpha, I-kappa-B kinase 1, IKK-A, IKK-alpha, IKK1, IkBKA, IkappaB kinase, Inhibitor of nuclear factor kappa-B kinase subunit alpha, NFKBIKA, Nuclear factor NF-kappa-B inhibitor kinase alpha, TCF16|
|Pathways||PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades|
Application DetailsProduct Details anti-CHUK Antibody Target Details CHUK Handling Images back to top
ELISA. Western Blot: 1: 1000 to 1: 2000 dilution. An 85 kDa band should be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CHUK Antibody Target Details CHUK Application Details Images back to top
|Buffer||PBS containing 0.02 % sodium azide.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|