anti-Rat (Rattus) Cyclin D1 antibody for Immunocytochemistry

Recommended Cyclin D1 Antibody (supplied by: Log in to see )

Antigen
Cyclin D1 (CCND1) Antibodies
  • BCL1
  • D11S287E
  • PRAD1
  • U21B31
  • AI327039
  • CycD1
  • Cyl-1
  • bcl-1
  • cD1
  • cb161
  • cycd1
  • etID37810.7
  • fb52e01
  • fc45c08
  • fc83a12
  • id:ibd1198
  • wu:fb52e01
  • wu:fc45c08
  • wu:fc83a12
  • ccnd1b
  • prad1
  • ccnd1
  • ccnd1a
  • bcl1
  • d11s287e
  • u21b31
  • CCND1
  • cyclin D1
  • cyclin D1 S homeolog
  • cyclin D1 L homeolog
  • cyclin D2
  • CCND1
  • Ccnd1
  • ccnd1
  • ccnd1.S
  • ccnd1.L
  • CCND2
Reactivity
Human, Mouse (Murine), Rat (Rattus)
514
275
248
44
38
17
17
16
15
4
4
4
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
Host
Rabbit
357
238
15
6
Clonality
Polyclonal
Conjugate
This Cyclin D1 antibody is un-conjugated
31
27
17
13
9
7
7
4
4
4
4
4
4
4
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
538
196
156
148
143
123
100
77
47
44
22
8
7
6
3
3
1
1
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Catalog No. ABIN6261145
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN3043639 ICC IHC (fro) IHC (p) WB Mouse IgG2a Log in to see DCS-6 23
1 ABIN5945958 ICC IF IHC IHC (p) IP WB Rabbit IgG C-Term Log in to see SA38-08 0
1 ABIN6261146 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 1
1 ABIN574432 ELISA FACS ICC IHC IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see CD1-1 0
1 ABIN4301536 ICC IF IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1077957 ICC IHC WB Rabbit IgG AA 1-295 Log in to see Polyclonal 0
1 ABIN490790 ELISA ICC IHC IP WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN6271700 ELISA ICC IF WB Rabbit IgG pThr288 Log in to see Polyclonal 0
1 ABIN6256534 ELISA ICC IF IHC WB Rabbit IgG pThr286 Log in to see Polyclonal 0
1 ABIN6271254 ELISA ICC IF IHC WB Rabbit IgG pSer90 Log in to see Polyclonal 0
1 ABIN6256448 ELISA ICC IF WB Rabbit IgG pSer90 Log in to see Polyclonal 0
1 ABIN2471067 ICC FACS IP IHC ELISA WB Mouse IgG1 Log in to see CD1-1 0
1 ABIN1501952 ICC IHC IHC (fro) IHC (p) WB Mouse IgG2a Log in to see CYD1 0
1 ABIN609471 ICC IHC IHC (fro) IHC (p) WB Mouse IgG2a Log in to see 0
1 ABIN4301541 FACS ICC IF IHC (p) IP WB Mouse IgG2a kappa Log in to see SPM587 0
1 ABIN200703 ICC IP WB Mouse IgG1 Log in to see 0
1 ABIN211137 Func ICC IP WB Rabbit IgG AA 285-295 Log in to see Polyclonal 0
1 ABIN4903416 ICC IHC IP WB Rabbit IgG Log in to see 0
1 ABIN4301564 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG2a kappa Log in to see SPM587 0
1 ABIN4301571 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 488 Mouse IgG2a kappa Log in to see SPM587 0

General

Antigen Cyclin D1 (CCND1) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(514), (275), (248), (44), (38), (17), (17), (16), (15), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(357), (238), (15), (6)
Clonality
Polyclonal
Conjugate This Cyclin D1 antibody is un-conjugated
(31), (27), (17), (13), (9), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(538), (196), (156), (148), (143), (123), (100), (77), (47), (44), (22), (8), (7), (6), (3), (3), (1), (1)
Pubmed 10 references available
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Product Details anti-Cyclin D1 Antibody

Target Details Cyclin D1 Application Details Handling References for anti-Cyclin D1 antibody (ABIN6261145) Images
Specificity Cyclin D1 Antibody detects endogenous levels of total Cyclin D1
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Immunogen A synthesized peptide derived from human Cyclin D1
Isotype IgG
Plasmids, Primers & others

Target Details Cyclin D1

Product Details anti-Cyclin D1 Antibody Application Details Handling References for anti-Cyclin D1 antibody (ABIN6261145) Images back to top
Antigen
Alternative Name Cyclin D1 (CCND1 Antibody Abstract)
Background

Description: Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.

Gene: CCND1

Molecular Weight 33kDa
Gene ID 595
UniProt P24385
Pathways PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling

Application Details

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Handling References for anti-Cyclin D1 antibody (ABIN6261145) Images back to top
Application Notes WB 1:500-1:2000 IHC 1:50-1:200 IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details References for anti-Cyclin D1 antibody (ABIN6261145) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

References for anti-Cyclin D1 antibody (ABIN6261145)

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Handling Images back to top
Product cited in:

Zhang, Wang, Miao, Li, Chen, Li: "Enhancing E-cadherin expression via promoter-targeted miR-373 suppresses bladder cancer cells growth and metastasis." in: Oncotarget, Vol. 8, Issue 55, pp. 93969-93983, 2018

Yuan, Wu, Li, Niu, Tan, Qiu: "Regulation of Signaling Pathways Involved in the Anti-proliferative and Apoptosis-inducing Effects of M22 against Non-small Cell Lung Adenocarcinoma A549 Cells." in: Scientific reports, Vol. 8, Issue 1, pp. 992, 2018

Li, Liu, Zhang, Cui, Ge, Wang, Chen: "Upregulation of E‑cadherin expression mediated by a novel dsRNA suppresses the growth and metastasis of bladder cancer cells by inhibiting β-catenin/TCF target genes." in: International journal of oncology, Vol. 52, Issue 6, pp. 1815-1826, 2018

Huang, Song, Tao, Shao, Zeng, Xu, Qi, Sun: "Ovostatin 2 knockdown significantly inhibits the growth, migration, and tumorigenicity of cutaneous malignant melanoma cells." in: PLoS ONE, Vol. 13, Issue 4, pp. e0195610, 2018

Shen, Yuan, Zhao, Li, Xu, Lou, Zheng, Bu, Guo, Xi: "Novel long non-coding RNA GACAT3 promotes gastric cancer cell proliferation through the IL-6/STAT3 signaling pathway." in: Tumour biology, Vol. 37, Issue 11, pp. 14895-14902, 2017

Li, Wang, Yu, Wu, Hu, Wang, Ye: "miR-3619-5p inhibits prostate cancer cell growth by activating CDKN1A expression." in: Oncology reports, Vol. 37, Issue 1, pp. 241-248, 2017

Yuan, Xi, Chen, Zhu, Kang, Zou, Wang, Bu: "STAT3 stimulates adipogenic stem cell proliferation and cooperates with HMGA2 during the early stage of differentiation to promote adipogenesis." in: Biochemical and biophysical research communications, Vol. 482, Issue 4, pp. 1360-1366, 2017

Li, Chen, Chen, Mo, Li, Xiao, Yu, Guo: "Circular RNA 0000096 affects cell growth and migration in gastric cancer." in: British journal of cancer, Vol. 116, Issue 5, pp. 626-633, 2017

Ge, Wang, Ruan, Chen, Liu, Ye: "Overexpression of p53 activated by small activating RNA suppresses the growth of human prostate cancer cells." in: OncoTargets and therapy, Vol. 9, pp. 231-41, 2016

Wang, Ge, Zhang, Chen, Hu, Li, Ye: "Targeted p53 activation by saRNA suppresses human bladder cancer cells growth and metastasis." in: Journal of experimental & clinical cancer research : CR, Vol. 35, pp. 53, 2016

Images

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Handling References for anti-Cyclin D1 antibody (ABIN6261145) back to top
Supplier Images
Western Blotting (WB) image for anti-Cyclin D1 (CCND1) antibody (ABIN6261145) Western blot analysis of Cyclin D1 expression in MCF7 whole cell lysates,The lane on ...
Western Blotting (WB) image for anti-Cyclin D1 (CCND1) antibody (ABIN6261145) estern blot analysis of Cyclin D1 expression in mouse brain tissue lysates,The lane o...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Cyclin D1 (CCND1) antibody (ABIN6261145) ABIN6267056 staining A-431 cells by IF/ICC. The sample were fixed with PFA and permea...