anti-Rat (Rattus) Cyclin D1 antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended Cyclin D1 Antibody (supplied by: Log in to see )

Antigen
Cyclin D1 (CCND1) Antibodies
  • BCL1
  • D11S287E
  • PRAD1
  • U21B31
  • AI327039
  • CycD1
  • Cyl-1
  • bcl-1
  • cD1
  • cb161
  • cycd1
  • etID37810.7
  • fb52e01
  • fc45c08
  • fc83a12
  • id:ibd1198
  • wu:fb52e01
  • wu:fc45c08
  • wu:fc83a12
  • ccnd1b
  • prad1
  • ccnd1
  • ccnd1a
  • bcl1
  • d11s287e
  • u21b31
  • CCND1
  • cyclin D1
  • cyclin D1 S homeolog
  • cyclin D1 L homeolog
  • cyclin D2
  • CCND1
  • Ccnd1
  • ccnd1
  • ccnd1.S
  • ccnd1.L
  • CCND2
Reactivity
Human, Monkey, Mouse (Murine), Rat (Rattus)
522
280
250
43
40
19
18
17
16
4
4
4
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
Host
Mouse
358
245
19
6
Clonality
Monoclonal
Conjugate
This Cyclin D1 antibody is conjugated to CF®660R
31
27
19
13
11
8
7
4
4
4
4
4
4
4
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
549
200
160
145
145
130
103
78
47
46
22
8
7
6
3
3
1
1
Supplier
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Catalog No. ABIN6161217
$ 209.23
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN6161219 FACS IF IHC (f) WB CF®568 Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161227 FACS IF IHC (f) WB CF®488A Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161223 FACS IF IHC (f) WB CF®680 Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161209 FACS IF IHC (f) WB CF®640R Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161207 FACS IF IHC (f) WB CF405M Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161211 FACS IF IHC (f) WB CF®543 Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161225 FACS IF IHC (f) WB CF®680R Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161229 FACS IF IHC (f) WB CF®594 Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161221 FACS IF IHC (f) WB CF®770 Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161205 FACS IF IHC (f) WB CF®405S Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161213 FACS IF IHC (f) WB CF®647 Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161215 FACS IF IHC (f) WB CF®555 Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161240 FACS IF IHC (f) WB Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161234 FACS IF IHC (f) WB Biotin Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161232 FACS IF IHC (f) WB Alkaline Phosphatase (AP) Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161231 FACS IF IHC (f) WB APC Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161238 FACS IF IHC (f) WB PerCP Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161239 FACS IF IHC (f) WB PE Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161242 FACS IF IHC (f) WB Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN302101 ELISA FACS ICC IHC (f) IHC (p) IP WB Mouse IgG1 Log in to see CD1-1 2

General

Antigen Cyclin D1 (CCND1) Antibodies
Reactivity Human, Monkey, Mouse (Murine), Rat (Rattus)
(522), (280), (250), (43), (40), (19), (18), (17), (16), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(358), (245), (19), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cyclin D1 antibody is conjugated to CF®660R
(31), (27), (19), (13), (11), (8), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
(549), (200), (160), (145), (145), (130), (103), (78), (47), (46), (22), (8), (7), (6), (3), (3), (1), (1)
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Product Details anti-Cyclin D1 Antibody

Target Details Cyclin D1 Application Details Handling Images
Purpose Mouse Monoclonal anti-Cyclin D1 (DCS-6), CF660R Conjugate
Characteristics Recognizes a protein of 36 kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60 % of mantle cell lymphomas (MCL) contain a t(11, 14)(q13, q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). About 40 % of breast carcinomas are positive for Cyclin D1. Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Immunogen Human full length recombinant cyclin D1 protein
Clone DCS-6
Isotype IgG2a, kappa
Plasmids, Primers & others

Target Details Cyclin D1

Product Details anti-Cyclin D1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CyclinD1 (CCND1 Antibody Abstract)
Molecular Weight 36 kDa
Gene ID 595
UniProt P24385
Pathways PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling

Application Details

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Handling Images back to top
Application Notes Immunohistology formalin-fixed 0.5-1 μg/mL
  • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
  • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
  • Immunofluorescence 1-2 μg/mL
  • Western blotting 0.5-1 μg/mL
  • Optimal dilution for a specific application should be determined by user
Comment

T47D, ZR75, BT474, SKBR3, MCF-7, MDA-MB-453, HT29, Ramos, Jurkat or A431 cells. Mantle cell lymphoma, breast or bladder carcinomas.
Restrictions For Research Use only

Handling

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Images back to top
Format Liquid
Concentration 100 μg/mL
Buffer PBS/0.1 % BSA/0.05 % azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light