anti-Rat (Rattus) Cyclin D1 antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended Cyclin D1 Antibody (supplied by: Log in to see )

Antigen
Cyclin D1 (CCND1) Antibodies
  • BCL1
  • D11S287E
  • PRAD1
  • U21B31
  • AI327039
  • CycD1
  • Cyl-1
  • bcl-1
  • cD1
  • cb161
  • cycd1
  • etID37810.7
  • fb52e01
  • fc45c08
  • fc83a12
  • id:ibd1198
  • wu:fb52e01
  • wu:fc45c08
  • wu:fc83a12
  • ccnd1b
  • prad1
  • ccnd1
  • ccnd1a
  • bcl1
  • d11s287e
  • u21b31
  • CCND1
  • cyclin D1
  • cyclin D1 S homeolog
  • cyclin D1 L homeolog
  • CCND1
  • Ccnd1
  • ccnd1
  • ccnd1.S
  • ccnd1.L
Reactivity
Human, Rat (Rattus)
513
274
248
44
38
17
17
16
15
4
4
4
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
Host
Mouse
357
237
15
6
Clonality
Monoclonal
Conjugate
This Cyclin D1 antibody is un-conjugated
31
27
17
13
9
7
7
4
4
4
4
4
4
4
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
537
196
157
149
142
122
99
77
46
44
22
8
7
6
3
3
1
1
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Catalog No. ABIN302101
$ 436.43
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.755764 ABIN4888512 FACS Func ICC IHC (f) IHC (p) IP WB PE Mouse IgG2a AA 1-295 Log in to see DCS-6 0
12.755764 ABIN4888511 FACS Func ICC IHC (f) IHC (p) IP WB Mouse IgG2a AA 1-295 Log in to see DCS-6 0
10 ABIN6161231 FACS IF IHC (f) WB APC Mouse IgG2a, kappa Log in to see DCS-6 0
10 ABIN6161238 FACS IF IHC (f) WB PerCP Mouse IgG2a, kappa Log in to see DCS-6 0
10 ABIN6161239 FACS IF IHC (f) WB PE Mouse IgG2a, kappa Log in to see DCS-6 0
10 ABIN6161242 FACS IF IHC (f) WB Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161215 FACS IF IHC (f) WB CF®555 Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161221 FACS IF IHC (f) WB CF®770 Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161225 FACS IF IHC (f) WB CF®680R Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161219 FACS IF IHC (f) WB CF®568 Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161205 FACS IF IHC (f) WB CF®405S Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161229 FACS IF IHC (f) WB CF®594 Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161217 FACS IF IHC (f) WB CF®660R Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161213 FACS IF IHC (f) WB CF®647 Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161207 FACS IF IHC (f) WB CF405M Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161209 FACS IF IHC (f) WB CF®640R Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161211 FACS IF IHC (f) WB CF®543 Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161223 FACS IF IHC (f) WB CF®680 Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161227 FACS IF IHC (f) WB CF®488A Mouse IgG2a, kappa Log in to see DCS-6 0
1 ABIN6161240 FACS IF IHC (f) WB Mouse IgG2a, kappa Log in to see DCS-6 0

General

Antigen Cyclin D1 (CCND1) Antibodies
Reactivity Human, Rat (Rattus)
(513), (274), (248), (44), (38), (17), (17), (16), (15), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(357), (237), (15), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cyclin D1 antibody is un-conjugated
(31), (27), (17), (13), (9), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(537), (196), (157), (149), (142), (122), (99), (77), (46), (44), (22), (8), (7), (6), (3), (3), (1), (1)
Pubmed 2 references available
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Product Details anti-Cyclin D1 Antibody

Target Details Cyclin D1 Application Details Handling References for anti-Cyclin D1 antibody (ABIN302101) Images
Specificity The antibody CD1.1 recognizes cyclin D1, an ubiquitously expressed 33 kDa protein that migrates as a 36 kDa band under reducing SDS-PAGE conditions.
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Immunogen Purified cyclin D1 protein
Clone CD1-1
Isotype IgG1
Plasmids, Primers & others

Target Details Cyclin D1

Product Details anti-Cyclin D1 Antibody Application Details Handling References for anti-Cyclin D1 antibody (ABIN302101) Images back to top
Antigen
Alternative Name Cyclin D1 (CCND1 Antibody Abstract)
Background Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest.
Pathways PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling

Application Details

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Handling References for anti-Cyclin D1 antibody (ABIN302101) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details References for anti-Cyclin D1 antibody (ABIN302101) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-Cyclin D1 antibody (ABIN302101)

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Handling Images back to top
Background publications

Diehl, Cheng, Roussel, Sherr: "Glycogen synthase kinase-3beta regulates cyclin D1 proteolysis and subcellular localization." in: Genes & development, Vol. 12, Issue 22, pp. 3499-511, 1999

Diehl, Zindy, Sherr: "Inhibition of cyclin D1 phosphorylation on threonine-286 prevents its rapid degradation via the ubiquitin-proteasome pathway." in: Genes & development, Vol. 11, Issue 8, pp. 957-72, 1997