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Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
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12.811332 | ABIN4888511 | FACS Func ICC IHC (f) IHC (p) IP WB | Mouse | IgG2a | AA 1-295 | Log in to see | DCS-6 | 0 | ||
12.811332 | ABIN4888512 | FACS Func ICC IHC (f) IHC (p) IP WB | PE | Mouse | IgG2a | AA 1-295 | Log in to see | DCS-6 | 0 | |
10 | ABIN6161231 | FACS IF IHC (f) WB | APC | Mouse | IgG2a, kappa | Log in to see | DCS-6 | 0 | ||
10 | ABIN6161238 | FACS IF IHC (f) WB | PerCP | Mouse | IgG2a, kappa | Log in to see | DCS-6 | 0 | ||
10 | ABIN6161239 | FACS IF IHC (f) WB | PE | Mouse | IgG2a, kappa | Log in to see | DCS-6 | 0 | ||
10 | ABIN6161242 | FACS IF IHC (f) WB | Mouse | IgG2a, kappa | Log in to see | DCS-6 | 0 | |||
1 | ABIN6161207 | FACS IF IHC (f) WB | CF405M | Mouse | IgG2a, kappa | Log in to see | DCS-6 | 0 | ||
1 | ABIN6161221 | FACS IF IHC (f) WB | CF®770 | Mouse | IgG2a, kappa | Log in to see | DCS-6 | 0 | ||
1 | ABIN6161223 | FACS IF IHC (f) WB | CF®680 | Mouse | IgG2a, kappa | Log in to see | DCS-6 | 0 | ||
1 | ABIN6161227 | FACS IF IHC (f) WB | CF®488A | Mouse | IgG2a, kappa | Log in to see | DCS-6 | 0 | ||
1 | ABIN6161209 | FACS IF IHC (f) WB | CF®640R | Mouse | IgG2a, kappa | Log in to see | DCS-6 | 0 | ||
1 | ABIN6161213 | FACS IF IHC (f) WB | CF®647 | Mouse | IgG2a, kappa | Log in to see | DCS-6 | 0 | ||
1 | ABIN6161217 | FACS IF IHC (f) WB | CF®660R | Mouse | IgG2a, kappa | Log in to see | DCS-6 | 0 | ||
1 | ABIN6161211 | FACS IF IHC (f) WB | CF®543 | Mouse | IgG2a, kappa | Log in to see | DCS-6 | 0 | ||
1 | ABIN6161215 | FACS IF IHC (f) WB | CF®555 | Mouse | IgG2a, kappa | Log in to see | DCS-6 | 0 | ||
1 | ABIN6161219 | FACS IF IHC (f) WB | CF®568 | Mouse | IgG2a, kappa | Log in to see | DCS-6 | 0 | ||
1 | ABIN6161225 | FACS IF IHC (f) WB | CF®680R | Mouse | IgG2a, kappa | Log in to see | DCS-6 | 0 | ||
1 | ABIN6161205 | FACS IF IHC (f) WB | CF®405S | Mouse | IgG2a, kappa | Log in to see | DCS-6 | 0 | ||
1 | ABIN6161229 | FACS IF IHC (f) WB | CF®594 | Mouse | IgG2a, kappa | Log in to see | DCS-6 | 0 | ||
1 | ABIN6161240 | FACS IF IHC (f) WB | Mouse | IgG2a, kappa | Log in to see | DCS-6 | 0 |
General |
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Antigen | Cyclin D1 (CCND1) Antibodies |
Reactivity | Human, Rat (Rattus) Alternatives |
Host | Mouse Alternatives |
Clonality (Clone) | |
Conjugate | This Cyclin D1 antibody is un-conjugated Alternatives |
Application |
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
Alternatives
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2 references available |
Supplier | Log in to see |
Product Details anti-Cyclin D1 AntibodyTarget Details Cyclin D1 Application Details Handling References for anti-Cyclin D1 antibody (ABIN302101) Images |
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Specificity | The antibody CD1.1 recognizes cyclin D1, an ubiquitously expressed 33 kDa protein that migrates as a 36 kDa band under reducing SDS-PAGE conditions. |
Cross-Reactivity (Details) | Human, Rat, Other not determined |
Purification | Purified by protein-A affinity chromatography |
Purity | > 95 % (by SDS-PAGE) |
Immunogen | Purified cyclin D1 protein |
Clone | CD1-1 |
Isotype | IgG1 |
Plasmids, Primers & others | |
Target Details Cyclin D1Product Details anti-Cyclin D1 Antibody Application Details Handling References for anti-Cyclin D1 antibody (ABIN302101) Images back to top |
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Antigen | |
Alternative Name | Cyclin D1 (CCND1 Antibody Abstract) |
Background | Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest. |
Pathways | PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling |
Application DetailsProduct Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Handling References for anti-Cyclin D1 antibody (ABIN302101) Images back to top |
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Application Notes | Optimal working dilution should be determined by the investigator. |
Restrictions | For Research Use only |
HandlingProduct Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details References for anti-Cyclin D1 antibody (ABIN302101) Images back to top |
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Concentration | 1 mg/mL |
Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide. |
Preservative | Sodium azide |
Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handling Advice | Do not freeze. |
Storage | 4 °C |
Storage Comment | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
References for anti-Cyclin D1 antibody (ABIN302101)Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Handling Images back to top |
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Background publications |
Diehl, Cheng, Roussel, Sherr: "Glycogen synthase kinase-3beta regulates cyclin D1 proteolysis and subcellular localization." in: Genes & development, Vol. 12, Issue 22, pp. 3499-511, 1999 Diehl, Zindy, Sherr: "Inhibition of cyclin D1 phosphorylation on threonine-286 prevents its rapid degradation via the ubiquitin-proteasome pathway." in: Genes & development, Vol. 11, Issue 8, pp. 957-72, 1997 |