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|Antigen||Huntingtin (HTT) Antibodies|
|Conjugate||This Huntingtin antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
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Product Details anti-Huntingtin AntibodyTarget Details Huntingtin Application Details Handling Images
|Specificity||Amino acids 416 to 424 of Human huntingtin protein phosphorylated at Ser421|
Synthetic peptide from human HTT / Huntingtin.
Type of Immunogen: Synthetic peptide
Target Details HuntingtinProduct Details anti-Huntingtin Antibody Application Details Handling Images back to top
|Alternative Name||HTT / Huntingtin (HTT Antibody Abstract)|
Name/Gene ID: HTT
Synonyms: HTT, Huntingtin, Huntington disease protein, IT15, HD, HD protein
|Pathways||PI3K-Akt Signaling, Hormone Transport, Transition Metal Ion Homeostasis, Tube Formation, Protein targeting to Nucleus, Dicarboxylic Acid Transport|
Application DetailsProduct Details anti-Huntingtin Antibody Target Details Huntingtin Handling Images back to top
Approved: ELISA (1:10000 - 1:40000), IHC-P (10 - 20 μg/mL), WB (1:500 - 1:3000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10-20 μg/mL.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Huntingtin Antibody Target Details Huntingtin Application Details Images back to top
|Buffer||0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeat freeze-thaw cycles.|
|Storage||4 °C,-20 °C|
Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.
ImagesProduct Details anti-Huntingtin Antibody Target Details Huntingtin Application Details Handling back to top