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|Antigen||Huntingtin (HTT) Antibodies|
|Epitope||AA 997-1276 Alternatives|
|Reactivity||Human, Mouse (Murine), Rabbit Alternatives|
|Conjugate||This Huntingtin antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
|2 references available|
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Product Details anti-Huntingtin AntibodyTarget Details Huntingtin Application Details Handling References for anti-Huntingtin antibody (ABIN119305) Images
|Specificity||This antibody reacts with an epitope corresponding to the HDA region (amino acids 1171 - 1177) of the huntingtin protein. Clone HDA3E10 detects a 350KD band on western blots but also detects smaller degradation products of huntingtin. The combined use of clone HDA3E10 (SM1660) HDC8A4 (SM1662) and HDB4E10 (SM1661) demonstrate that huntingtin is enriched in neuronal cells in the brain.|
|Immunogen||Recombinant protein corresponding to amino acids 997 -1276 of huntingtin|
Target Details HuntingtinProduct Details anti-Huntingtin Antibody Application Details Handling References for anti-Huntingtin antibody (ABIN119305) Images back to top
|Alternative Name||Huntingtin (HTT Antibody Abstract)|
|Background||Huntingtin is a 350 kDa protein of 3144 amino acids that is altered in Huntington's disease (HD). The expanded trinucleotide CAG repeat of the HD gene encodes an abnormally expanded polyglutamine stretch in the N-terminus of the Huntingtin protein. The normal function of Huntingtin still remains uncertain but it has been suggested that it performs a necessary housekeeping function. The abnormal form of Huntingtin aggregates in vitro and forms neuronal intranuclear and cytoplasmic inclusions in HD patients. The expanded polyglutamine repeats formed by this mutation of the Huntingtin gene have been proposed to cause neuronal degeneration in HD through abnormal interactions with other proteins containing short polyglutamine tracts such as the CREB binding protein (CBP), a protein that promotes cell survival and is a major mediator of survival signals in mature neurons.Synonyms: HD, HTT, Huntington Disease Protein, IT15|
|Pathways||PI3K-Akt Signaling, Hormone Transport, Transition Metal Ion Homeostasis, Tube Formation, Protein targeting to Nucleus, Dicarboxylic Acid Transport|
Application DetailsProduct Details anti-Huntingtin Antibody Target Details Huntingtin Handling References for anti-Huntingtin antibody (ABIN119305) Images back to top
Western blotting. Immunoprecipitation. Immunohistochemistry on frozen sections, recommended positive control tissue: Brain (Increased cytoplasmic staining, relative to nuclear, has been reported using formaldehyde as a fixative compared with acetone/methanol(1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Huntingtin Antibody Target Details Huntingtin Application Details References for anti-Huntingtin antibody (ABIN119305) Images back to top
|Buffer||PBS buffer with 0.09 % sodium azide as preservative|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-Huntingtin antibody (ABIN119305)Product Details anti-Huntingtin Antibody Target Details Huntingtin Application Details Handling Images back to top
Nucifora, Sasaki, Peters, Huang, Cooper, Yamada, Takahashi, Tsuji, Troncoso, Dawson, Dawson, Ross: "Interference by huntingtin and atrophin-1 with cbp-mediated transcription leading to cellular toxicity." in: Science (New York, N.Y.), Vol. 291, Issue 5512, pp. 2423-8, 2001
Wilkinson, Nguyen, Manilal, Thomas, Neal, Harper, Jones, Morris: "Localization of rabbit huntingtin using a new panel of monoclonal antibodies." in: Brain research. Molecular brain research, Vol. 69, Issue 1, pp. 10-20, 1999
ImagesProduct Details anti-Huntingtin Antibody Target Details Huntingtin Application Details Handling References for anti-Huntingtin antibody (ABIN119305) back to top