anti-Dog (Canine) MAP3K8 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended MAP3K8 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) Antibodies
  • MAP3K8
  • COT
  • EST
  • ESTF
  • MEKK8
  • TPL2
  • Tpl-2
  • c-COT
  • Cot
  • Cot/Tpl2
  • Est
  • Estf
  • Tpl2
  • D17TPL2
  • mitogen-activated protein kinase kinase kinase 8
  • MAP3K8
  • map3k8
  • Map3k8
Epitope
C-Term
17
15
13
9
9
8
6
5
4
4
4
3
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Bat, Cow (Bovine), Dog (Canine), Human, Monkey
116
55
52
10
7
4
3
3
1
1
1
Host
Rabbit
112
4
Clonality
Polyclonal
Conjugate
This MAP3K8 antibody is un-conjugated
6
6
5
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
66
51
50
34
15
11
11
5
4
3
3
2
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Catalog No. ABIN1049034
$ 484.00
Plus shipping costs $45.00

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) Antibodies
Epitope C-Term
(17), (15), (13), (9), (9), (8), (6), (5), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Bat, Cow (Bovine), Dog (Canine), Human, Monkey
(116), (55), (52), (10), (7), (4), (3), (3), (1), (1), (1)
Host Rabbit
(112), (4)
Clonality
Polyclonal
Conjugate This MAP3K8 antibody is un-conjugated
(6), (6), (5), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(66), (51), (50), (34), (15), (11), (11), (5), (4), (3), (3), (2)
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Product Details anti-MAP3K8 Antibody

Target Details MAP3K8 Application Details Handling Images
Brand IHC-plus™
Specificity Human MAP3K8. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Elephant, Bovine, Dog, Bat (100%) Mouse, Rat, Hamster, Panda, Horse (94%).
Purification Immunoaffinity purified
Immunogen Synthetic 18 amino acid peptide from near C-terminus of human MAP3K8. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Elephant, Bovine, Dog, Bat (100%), Mouse, Rat, Hamster, Panda, Horse (94%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details MAP3K8

Product Details anti-MAP3K8 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAP3K8 / TPL2 (MAP3K8 Antibody Abstract)
Background Name/Gene ID: MAP3K8
Subfamily: MAP3K
Family: Protein Kinase

Synonyms: MAP3K8, C-COT, Cancer Osaka thyroid oncogene, COT, Ewing sarcoma transformant, EST, MEKK8, Proto-oncogene c-Cot, TPL2, Tumor progression locus 2, Protein kinase cot, Tpl-2, ESTF
Gene ID 1326
Pathways PI3K-Akt Signaling, TCR Signaling

Application Details

Product Details anti-MAP3K8 Antibody Target Details MAP3K8 Handling Images back to top
Application Notes Approved: IHC, IHC-P (10 μg/mL)
Comment

Target Species of Antibody: Human

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-MAP3K8 Antibody Target Details MAP3K8 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-MAP3K8 Antibody Target Details MAP3K8 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) (C-Term) antibody (ABIN1049034) Anti-MAP3K8 antibody ABIN1049034 IHC staining of human colon. Immunohistochemistry o...