anti-Mouse (Murine) MAP3K8 antibody for Immunoprecipitation

Recommended MAP3K8 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) Antibodies
  • MAP3K8
  • COT
  • EST
  • ESTF
  • MEKK8
  • TPL2
  • Tpl-2
  • c-COT
  • Cot
  • Cot/Tpl2
  • Est
  • Estf
  • Tpl2
  • D17TPL2
  • mitogen-activated protein kinase kinase kinase 8
  • MAP3K8
  • map3k8
  • Map3k8
Epitope
C-Term
17
15
13
10
9
9
6
5
5
4
4
3
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
121
56
53
11
8
4
4
3
2
1
1
Host
Rabbit
115
6
Clonality
Polyclonal
Conjugate
This MAP3K8 antibody is un-conjugated
7
6
5
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Dot Blot (DB), ELISA, Immunoprecipitation (IP), Western Blotting (WB)
70
52
52
36
15
12
11
5
5
3
3
2
Supplier
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Catalog No. ABIN4332655
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2746879 ELISA IP WB FITC Rabbit AA 360-470 Log in to see Polyclonal 0
1 ABIN2746878 ELISA IHC IP WB Rabbit AA 360-470 Log in to see Polyclonal 0
1 ABIN5558737 ELISA IP WB Biotin Rabbit Log in to see Polyclonal 0

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) Antibodies
Epitope C-Term
(17), (15), (13), (10), (9), (9), (6), (5), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(121), (56), (53), (11), (8), (4), (4), (3), (2), (1), (1)
Host Rabbit
(115), (6)
Clonality
Polyclonal
Conjugate This MAP3K8 antibody is un-conjugated
(7), (6), (5), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Dot Blot (DB), ELISA, Immunoprecipitation (IP), Western Blotting (WB)
(70), (52), (52), (36), (15), (12), (11), (5), (5), (3), (3), (2)
Pubmed 4 references available
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Product Details anti-MAP3K8 Antibody

Target Details MAP3K8 Application Details Handling References for anti-MAP3K8 antibody (ABIN4332655) Images
Specificity This antibody detects a single band of approximately 57 kDa in PC-MAP3K8 samples
Purification Immunogen affinity purified
Immunogen Synthetic peptides corresponding to unique epitope on MAP3K8. The peptide sequence was selected from C-terminal-region, amimo acids 365-467. This peptide was covalently modified post-synthetically to achieve desired antigenicity.
Plasmids, Primers & others

Target Details MAP3K8

Product Details anti-MAP3K8 Antibody Application Details Handling References for anti-MAP3K8 antibody (ABIN4332655) Images back to top
Antigen
Alternative Name MAP3K8/Tpl2/COT (MAP3K8 Antibody Abstract)
Background Gene Symbol: MAP3K8
Gene ID 1326
Pathways PI3K-Akt Signaling, TCR Signaling

Application Details

Product Details anti-MAP3K8 Antibody Target Details MAP3K8 Handling References for anti-MAP3K8 antibody (ABIN4332655) Images back to top
Application Notes Western Blot > 1:500, ELISA < 1:10000, Immunoprecipitation 1:200, Dot Blot 1:10000By western blot this antibody detects a single band of approximately 57 kDa in PC-MAP3K8 samples.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-MAP3K8 Antibody Target Details MAP3K8 Application Details References for anti-MAP3K8 antibody (ABIN4332655) Images back to top
Format Liquid
Buffer Tris/Glycine buffer pH 7.5-7.9, stabilizing protein, and glycerol
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.

References for anti-MAP3K8 antibody (ABIN4332655)

Product Details anti-MAP3K8 Antibody Target Details MAP3K8 Application Details Handling Images back to top
Product cited in:

Jeong, Bhatia, Toth, Oh, Inn, Liao, Roy-Burman, Melamed, Coetzee, Jung: "TPL2/COT/MAP3K8 (TPL2) activation promotes androgen depletion-independent (ADI) prostate cancer growth." in: PLoS ONE, Vol. 6, Issue 1, pp. e16205, 2011

Jager, Grémeaux, Gonzalez, Bonnafous, Debard, Laville, Vidal, Tran, Gual, Le Marchand-Brustel, Cormont, Tanti: "Tpl2 kinase is upregulated in adipose tissue in obesity and may mediate interleukin-1beta and tumor necrosis factor-{alpha} effects on extracellular signal-regulated kinase activation and lipolysis." in: Diabetes, Vol. 59, Issue 1, pp. 61-70, 2009

Mielke, Elkins, Wei, Starr, Tsichlis, OShea, Watford: "Tumor progression locus 2 (Map3k8) is critical for host defense against Listeria monocytogenes and IL-1 beta production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 12, pp. 7984-93, 2009

ONeill: "When signaling pathways collide: positive and negative regulation of toll-like receptor signal transduction." in: Immunity, Vol. 29, Issue 1, pp. 12-20, 2008

Images

Product Details anti-MAP3K8 Antibody Target Details MAP3K8 Application Details Handling References for anti-MAP3K8 antibody (ABIN4332655) back to top
Supplier Images
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) (C-Term) antibody (ABIN4332655) Western Blot: MAP3K8/Tpl2/COT Antibody [NBP1-62178] - 50-60 ug of rat midbrain protei...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) (C-Term) antibody (ABIN4332655) Immunohistochemistry-Paraffin: MAP3K8/Tpl2/COT Antibody - 1:1000 dilution in IHC Blo...