FETUB Protein (AA 16-382) (His tag)

Catalog No. ABIN1096645

Product Details

Target: FETUB (Fetuin B (FETUB))

Protein Type: Recombinant

Protein Characteristics: AA 16-382

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This FETUB protein is labelled with His tag.

Purpose: Recombinant Human Fetuin-B/FETUB (C-6His)

Sequence: CGAMSPPQLA LNPSALLSRG CNDSDVLAVA GFALRDINKD RKDGYVLRLN RVNDAQEYRR GGLGSLFYLT LDVLETDCHV LRKKAWQDCG MRIFFESVYG QCKAIFYMNN PSRVLYLAAY NCTLRPVSKK KIYMTCPDCP SSIPTDSSNH QVLEAATESL AKYNNENTSK QYSLFKVTRA SSQWVVGPSY FVEYLIKESP CTKSQASSCS LQSSDSVPVG LCKGSLTRTH WEKFVSVTCD FFESQAPATG SENSAVNQKP TNLPKVEESQ QKNTPPTDSP SKAGPRGSVQ YLPDLDDKNS QEKGPQEAFP VHLDLTTNPQ GETLDISFLF LEPMEEKLVV LPFPKEKART AECPGPAQNA SPLVLPPVDH HHHHH

Characteristics: Recombinant Human Fetuin-B is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Cys16-Pro382) of Human FETUB fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: FETUB (Fetuin B (FETUB))

Alternative Name: fetuin-b (FETUB Products)

Synonyms: 16G2 Protein, Gugu Protein, IRL685 Protein, Fet Protein, Pp63 Protein, gugu Protein, fetuin-b Protein, FETUB Protein, 16g2 Protein, irl685 Protein, im:6910148 Protein, 2310011O17Rik Protein, AI255764 Protein, D17980 Protein, fetuin B Protein, fetuin B L homeolog Protein, fetuin beta Protein, FETUB Protein, Fetub Protein, fetub.L Protein, fetub Protein

Sub Type: Fusionprotein

Background: Fetuin-B is a member of the Fetuin family that is part of the Cystatin superfamily of Cysteine Protease inhibitors. It is reported that Fetuin-B is highly expressed in liver tissue, in tongue and placenta tissues. Fetuin-B is a paralogue of Fetuin-A. Fetuin-A and Fetuin-B display similarities and differences in their characteristics, however, they share only 20% amino acid sequence identity. The amounts of Fetuin-B in human serum, unlike Fetuin-A, vary with gender and are higher in females than in males. Fetuin-B is an inhibitor of basic calcium phosphate precipitation but is less active than Fetuin-A. Fetuin-B expression is decreased in Fetuin-A deficient knock-out mice. The expression of Fetuin-B has been shown to be regulated by FXR (Farnesoid X Receptor), a nuclear receptor activated by bile acids. Evidence has shown that overexpression of Fetuin-B in skin squamous carcinoma cells suppresses tumor growth in nude mice. The function of Fetuin B is still not fully characterized.
Alternative Names: Fetuin-B, 16G2, Fetuin-Like Protein IRL685, Gugu, FETUB

Molecular Weight: 41.53 kDa

UniProt: Q9UGM5